Mutagenetix > Incidental Mutation

Incidental Mutation 'R4429:Vmn1r174'

328321

Institutional Source

Beutler Lab

Gene Symbol

Vmn1r174

Ensembl Gene

ENSMUSG00000090411

Gene Name

vomeronasal 1 receptor 174

Synonyms

V1rd22

MMRRC Submission

041699-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.051) question?

Stock #

R4429 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

23453336-23454277 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 23453565 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 77 (V77A)

Ref Sequence

ENSEMBL: ENSMUSP00000154506 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000167551] [ENSMUST00000228331]

AlphaFold

E9PYW5

Predicted Effect

probably benign
Transcript: ENSMUST00000167551
AA Change: V77A

PolyPhen 2 Score 0.020 (Sensitivity: 0.95; Specificity: 0.80)

SMART Domains

Protein: ENSMUSP00000126478
Gene: ENSMUSG00000090411
AA Change: V77A

Domain	Start	End	E-Value	Type
Pfam:TAS2R	8	300	4e-9	PFAM
Pfam:V1R	43	300	5e-19	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000228331
AA Change: V77A

PolyPhen 2 Score 0.020 (Sensitivity: 0.95; Specificity: 0.80)

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.3%
20x: 95.5%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	G	T	5: 64,056,182 (GRCm39)		probably benign	Het
Abca5	T	C	11: 110,202,236 (GRCm39)	T390A	probably benign	Het
Ahcyl2	T	G	6: 29,894,874 (GRCm39)	V452G	probably damaging	Het
Ano4	T	A	10: 88,828,804 (GRCm39)	N545I	probably damaging	Het
Bag4	C	T	8: 26,259,516 (GRCm39)	A228T	probably benign	Het
Catip	T	A	1: 74,407,891 (GRCm39)		probably benign	Het
Chrna4	A	G	2: 180,670,413 (GRCm39)	S448P	probably damaging	Het
Cldn8	A	C	16: 88,359,619 (GRCm39)	M102R	probably damaging	Het
Cnpy3	T	A	17: 47,058,070 (GRCm39)	Q111L	probably benign	Het
Dnah8	A	G	17: 30,971,120 (GRCm39)	N2725D	probably damaging	Het
Dock8	T	C	19: 25,042,754 (GRCm39)	V112A	probably benign	Het
Ephb3	G	A	16: 21,033,213 (GRCm39)	E66K	probably damaging	Het
Gm6594	T	A	17: 82,846,923 (GRCm39)	D79E	probably benign	Het
Gtf2e2	T	A	8: 34,242,521 (GRCm39)	Y74*	probably null	Het
Hacd4	A	T	4: 88,353,184 (GRCm39)	F103I	possibly damaging	Het
Hap1	T	C	11: 100,245,098 (GRCm39)	T38A	probably benign	Het
Havcr2	T	A	11: 46,347,387 (GRCm39)	D72E	probably damaging	Het
Iqcg	G	A	16: 32,839,860 (GRCm39)	T362I	probably benign	Het
Lect2	C	T	13: 56,693,538 (GRCm39)		probably null	Het
Lemd3	T	C	10: 120,813,893 (GRCm39)	T447A	probably benign	Het
Lrrc72	T	C	12: 36,258,623 (GRCm39)	N78S	probably damaging	Het
Map3k14	A	G	11: 103,118,410 (GRCm39)	L592P	probably damaging	Het
Meioc	T	C	11: 102,566,546 (GRCm39)	Y721H	probably damaging	Het
Mrps10	T	A	17: 47,689,124 (GRCm39)		probably null	Het
Myo5c	A	G	9: 75,201,283 (GRCm39)	Y1406C	probably damaging	Het
Myo7a	T	C	7: 97,702,395 (GRCm39)	Y2098C	probably damaging	Het
Nol9	C	T	4: 152,125,631 (GRCm39)	T194I	probably damaging	Het
Nox3	G	A	17: 3,733,233 (GRCm39)	T206I	probably benign	Het
Nsd2	T	G	5: 34,000,546 (GRCm39)	M21R	probably damaging	Het
Pcdh9	T	C	14: 94,124,820 (GRCm39)	N327S	probably damaging	Het
Pclo	T	G	5: 14,728,114 (GRCm39)		probably benign	Het
Pparg	T	A	6: 115,416,984 (GRCm39)	M59K	probably benign	Het
Prag1	A	G	8: 36,613,796 (GRCm39)	K1116R	probably damaging	Het
Rhbdf1	C	T	11: 32,163,369 (GRCm39)	E368K	probably benign	Het
Rita1	A	G	5: 120,747,626 (GRCm39)	V224A	probably damaging	Het
Rsph6a	T	A	7: 18,807,988 (GRCm39)	W384R	probably damaging	Het
Scn1a	A	T	2: 66,181,329 (GRCm39)	Y65N	possibly damaging	Het
Serpina5	C	A	12: 104,069,665 (GRCm39)	F292L	probably benign	Het
Sf3b3	A	C	8: 111,552,750 (GRCm39)	L511V	probably benign	Het
Siglecg	C	T	7: 43,067,350 (GRCm39)	P639L	possibly damaging	Het
Slc12a3	A	T	8: 95,069,713 (GRCm39)	I541F	probably damaging	Het
Slco6d1	T	C	1: 98,424,091 (GRCm39)	V581A	possibly damaging	Het
Sptbn2	T	C	19: 4,788,383 (GRCm39)	Y1121H	probably damaging	Het
Sytl5	A	T	X: 9,826,262 (GRCm39)	N412Y	probably damaging	Het
Timm29	G	C	9: 21,504,775 (GRCm39)	A148P	probably damaging	Het
Tmem181a	T	A	17: 6,346,061 (GRCm39)	L185H	probably damaging	Het
Tmem67	T	A	4: 12,051,473 (GRCm39)	N785I	possibly damaging	Het
Trhde	A	T	10: 114,339,028 (GRCm39)	L594Q	probably damaging	Het
Uba6	C	T	5: 86,268,406 (GRCm39)	V941I	probably damaging	Het
Zfp661	A	G	2: 127,420,628 (GRCm39)	V57A	probably damaging	Het
Zfp867	C	T	11: 59,355,863 (GRCm39)	D64N	possibly damaging	Het
Zp3r	T	C	1: 130,519,128 (GRCm39)	T294A	possibly damaging	Het

Other mutations in Vmn1r174

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00331:Vmn1r174	APN	7	23,453,958 (GRCm39)	missense	possibly damaging	0.77
IGL00950:Vmn1r174	APN	7	23,453,911 (GRCm39)	missense	possibly damaging	0.96
IGL01484:Vmn1r174	APN	7	23,453,749 (GRCm39)	nonsense	probably null
IGL02014:Vmn1r174	APN	7	23,453,583 (GRCm39)	missense	probably damaging	1.00
IGL02190:Vmn1r174	APN	7	23,454,252 (GRCm39)	missense	unknown
IGL03265:Vmn1r174	APN	7	23,453,898 (GRCm39)	nonsense	probably null
IGL03335:Vmn1r174	APN	7	23,453,937 (GRCm39)	missense	probably benign	0.41
R0529:Vmn1r174	UTSW	7	23,453,622 (GRCm39)	missense	probably benign	0.00
R1489:Vmn1r174	UTSW	7	23,453,981 (GRCm39)	nonsense	probably null
R1645:Vmn1r174	UTSW	7	23,453,777 (GRCm39)	missense	possibly damaging	0.87
R1691:Vmn1r174	UTSW	7	23,453,337 (GRCm39)	start codon destroyed	probably null	1.00
R1753:Vmn1r174	UTSW	7	23,453,622 (GRCm39)	missense	probably benign	0.00
R1939:Vmn1r174	UTSW	7	23,453,532 (GRCm39)	missense	probably damaging	0.99
R1988:Vmn1r174	UTSW	7	23,454,050 (GRCm39)	missense	probably damaging	0.98
R2299:Vmn1r174	UTSW	7	23,453,429 (GRCm39)	missense	probably benign	0.08
R4516:Vmn1r174	UTSW	7	23,453,768 (GRCm39)	missense	probably benign	0.01
R4589:Vmn1r174	UTSW	7	23,454,204 (GRCm39)	nonsense	probably null
R5175:Vmn1r174	UTSW	7	23,454,153 (GRCm39)	missense	probably benign	0.03
R5392:Vmn1r174	UTSW	7	23,454,227 (GRCm39)	missense	unknown
R5503:Vmn1r174	UTSW	7	23,453,562 (GRCm39)	missense	probably benign	0.03
R5568:Vmn1r174	UTSW	7	23,453,919 (GRCm39)	missense	probably damaging	0.96
R6705:Vmn1r174	UTSW	7	23,453,851 (GRCm39)	missense	probably benign	0.25
R8168:Vmn1r174	UTSW	7	23,454,096 (GRCm39)	missense	probably damaging	0.99
R8190:Vmn1r174	UTSW	7	23,453,568 (GRCm39)	missense	probably damaging	1.00
R8523:Vmn1r174	UTSW	7	23,454,182 (GRCm39)	missense	probably damaging	1.00
R8555:Vmn1r174	UTSW	7	23,453,970 (GRCm39)	missense	possibly damaging	0.84
R8913:Vmn1r174	UTSW	7	23,453,375 (GRCm39)	missense	possibly damaging	0.90
R8979:Vmn1r174	UTSW	7	23,453,892 (GRCm39)	missense	possibly damaging	0.92
R8990:Vmn1r174	UTSW	7	23,453,956 (GRCm39)	missense	possibly damaging	0.71
X0011:Vmn1r174	UTSW	7	23,453,906 (GRCm39)	missense	probably benign	0.03

Predicted Primers

PCR Primer
(F):5'- TGCCAGGTTGGTATTGCAGC -3'
(R):5'- TATTGTCTGTGTTCCATGGACC -3'

Sequencing Primer
(F):5'- TATTGCAGCTGTGGCCAAC -3'
(R):5'- CCATGGACCACTGATTTTTATAGGG -3'

Posted On

2015-07-07