Mutagenetix > Incidental Mutation

Incidental Mutation 'R3162:Cdc14b'

271694

Institutional Source

Beutler Lab

Gene Symbol

Cdc14b

Ensembl Gene

ENSMUSG00000033102

Gene Name

CDC14 cell division cycle 14B

Synonyms

A530086E13Rik, 2810432N10Rik

MMRRC Submission

040613-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.240) question?

Stock #

R3162 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

64337082-64423104 bp(-) (GRCm39)

Type of Mutation

splice site

DNA Base Change (assembly)

A to G at 64394422 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000152388 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000039318] [ENSMUST00000109769] [ENSMUST00000109770] [ENSMUST00000221139] [ENSMUST00000221634]

AlphaFold

Q6PFY9

Predicted Effect

probably benign
Transcript: ENSMUST00000039318

SMART Domains

Protein: ENSMUSP00000046003
Gene: ENSMUSG00000033102

Domain	Start	End	E-Value	Type
low complexity region	15	34	N/A	INTRINSIC
Pfam:DSPn	51	189	6.1e-57	PFAM
Pfam:DSPc	240	365	9.2e-17	PFAM
Pfam:Y_phosphatase	244	365	1e-7	PFAM
transmembrane domain	445	467	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000109769

SMART Domains

Protein: ENSMUSP00000105391
Gene: ENSMUSG00000033102

Domain	Start	End	E-Value	Type
Pfam:DSPn	12	152	2.5e-58	PFAM
Pfam:DSPc	203	328	8e-17	PFAM
Pfam:Y_phosphatase	206	328	8.9e-8	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000109770

SMART Domains

Protein: ENSMUSP00000105392
Gene: ENSMUSG00000033102

Domain	Start	End	E-Value	Type
low complexity region	15	34	N/A	INTRINSIC
Pfam:DSPn	51	189	3.4e-57	PFAM
Pfam:DSPc	240	365	2.8e-16	PFAM
Pfam:Y_phosphatase	252	364	2.4e-7	PFAM
transmembrane domain	445	467	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000181403

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000185652

Predicted Effect

probably benign
Transcript: ENSMUST00000221139

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000221437

Predicted Effect

probably benign
Transcript: ENSMUST00000221634

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000222277

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.0%

Validation Efficiency

98% (49/50)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. This protein is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, which suggests the role in cell cycle control. This protein has been shown to interact with and dephosphorylates tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splice of this gene results in 3 transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit premature aging, including premature cataracts and kyphosis; reduced fertility, particularly in female mice; and impaired contextual conditioning. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 89 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610040J01Rik	T	C	5: 64,053,833 (GRCm39)		probably benign	Het
4933412E24Rik	T	A	15: 59,888,134 (GRCm39)	E102V	probably damaging	Het
Adcy9	A	G	16: 4,129,452 (GRCm39)	L715P	probably damaging	Het
Adgre4	T	C	17: 56,109,218 (GRCm39)		probably benign	Het
Amer2	A	G	14: 60,616,000 (GRCm39)	D65G	probably damaging	Het
Atad2b	C	A	12: 4,989,689 (GRCm39)	N133K	possibly damaging	Het
AW551984	C	A	9: 39,504,325 (GRCm39)	R547L	probably damaging	Het
B3galt6	A	G	4: 156,076,464 (GRCm39)	Y204H	probably benign	Het
Btnl2	T	C	17: 34,577,039 (GRCm39)	W65R	probably damaging	Het
Camk1g	T	C	1: 193,042,115 (GRCm39)	T45A	possibly damaging	Het
Caps2	C	A	10: 112,018,391 (GRCm39)	Y180*	probably null	Het
Ccdc181	T	A	1: 164,107,865 (GRCm39)	S183T	probably damaging	Het
Cep350	T	C	1: 155,738,910 (GRCm39)	H2311R	probably benign	Het
Cfap54	T	A	10: 92,881,140 (GRCm39)	K349N	probably damaging	Het
Copa	T	A	1: 171,918,800 (GRCm39)	C127S	probably damaging	Het
Crbn	T	C	6: 106,767,827 (GRCm39)	Q221R	probably benign	Het
Dapk2	T	G	9: 66,161,893 (GRCm39)	V267G	probably damaging	Het
Ddb1	T	C	19: 10,603,335 (GRCm39)	L881P	probably damaging	Het
Decr1	T	A	4: 15,930,972 (GRCm39)	D120V	probably damaging	Het
Dennd1c	C	T	17: 57,373,562 (GRCm39)	G637D	possibly damaging	Het
Dhrs3	A	G	4: 144,646,016 (GRCm39)	D108G	possibly damaging	Het
Disp1	T	C	1: 182,868,806 (GRCm39)	K1205E	probably benign	Het
Dnajc13	G	T	9: 104,097,097 (GRCm39)	N510K	possibly damaging	Het
Dusp6	T	C	10: 99,099,944 (GRCm39)	Y131H	probably damaging	Het
Eif2b2	A	T	12: 85,266,435 (GRCm39)	M34L	probably benign	Het
Epsti1	A	T	14: 78,211,953 (GRCm39)		probably benign	Het
Errfi1	G	A	4: 150,951,816 (GRCm39)	E415K	probably damaging	Het
Ext1	T	C	15: 53,208,000 (GRCm39)	N254D	possibly damaging	Het
Gm7337	A	C	5: 87,999,416 (GRCm39)		noncoding transcript	Het
Hnrnpu	T	C	1: 178,158,690 (GRCm39)		probably benign	Het
Hyal3	T	A	9: 107,464,005 (GRCm39)	C407S	probably damaging	Het
Insr	T	G	8: 3,211,416 (GRCm39)	N1141T	possibly damaging	Het
Ipo9	T	C	1: 135,337,214 (GRCm39)	T174A	probably benign	Het
Iqgap1	G	A	7: 80,402,086 (GRCm39)	A393V	probably benign	Het
Irak2	G	T	6: 113,649,721 (GRCm39)	A119S	probably benign	Het
Itgb2l	A	G	16: 96,238,589 (GRCm39)	L70P	probably damaging	Het
Itsn1	C	A	16: 91,649,932 (GRCm39)	S202*	probably null	Het
Ivd	T	C	2: 118,692,650 (GRCm39)		probably null	Het
Leprot	C	T	4: 101,515,090 (GRCm39)	T89I	probably damaging	Het
Lratd2	A	T	15: 60,695,296 (GRCm39)	V150E	probably damaging	Het
Mill2	A	C	7: 18,590,099 (GRCm39)	E127A	probably benign	Het
Msh6	T	C	17: 88,292,909 (GRCm39)	Y555H	probably damaging	Het
Myo18b	A	C	5: 112,840,594 (GRCm39)	S2400A	probably damaging	Het
Naa25	A	G	5: 121,573,135 (GRCm39)		probably null	Het
Nop2	A	G	6: 125,111,555 (GRCm39)	N96S	probably benign	Het
Nup155	G	T	15: 8,177,867 (GRCm39)	R1083S	possibly damaging	Het
Nusap1	A	T	2: 119,460,885 (GRCm39)	Q126L	possibly damaging	Het
Or11g24	T	A	14: 50,662,488 (GRCm39)	C171S	probably damaging	Het
Or13c7b	T	A	4: 43,820,544 (GRCm39)	K272N	probably benign	Het
Or2z2	T	C	11: 58,346,053 (GRCm39)	T241A	probably damaging	Het
Or4c52	T	G	2: 89,845,365 (GRCm39)	Y30*	probably null	Het
Or5al1	T	C	2: 85,990,439 (GRCm39)	I92V	probably benign	Het
Or6x1	G	T	9: 40,098,901 (GRCm39)	Q163H	probably benign	Het
Or7a35	C	A	10: 78,853,438 (GRCm39)	T94N	probably benign	Het
Pde5a	T	A	3: 122,575,277 (GRCm39)	L356*	probably null	Het
Pdik1l	A	G	4: 134,011,561 (GRCm39)	L94S	probably damaging	Het
Pkdrej	T	A	15: 85,700,818 (GRCm39)	D1706V	probably damaging	Het
Pkhd1l1	A	G	15: 44,368,924 (GRCm39)	I856M	probably damaging	Het
Prkcz	A	T	4: 155,374,981 (GRCm39)	D114E	probably benign	Het
Prss59	A	G	6: 40,903,003 (GRCm39)	M123T	probably benign	Het
Psap	T	C	10: 60,113,575 (GRCm39)	L4P	possibly damaging	Het
Ptprk	T	C	10: 28,468,822 (GRCm39)	V1402A	probably benign	Het
Rai14	T	C	15: 10,633,250 (GRCm39)	T47A	possibly damaging	Het
Ralgapa1	A	T	12: 55,756,371 (GRCm39)	N1075K	probably damaging	Het
Rlf	A	G	4: 121,006,044 (GRCm39)	S979P	probably damaging	Het
Rps2	G	T	17: 24,939,952 (GRCm39)	A129S	probably benign	Het
Serinc2	A	G	4: 130,154,528 (GRCm39)	S175P	probably benign	Het
Skic2	C	T	17: 35,066,789 (GRCm39)	W88*	probably null	Het
Socs5	A	T	17: 87,442,146 (GRCm39)	Q362L	probably damaging	Het
Srbd1	A	T	17: 86,437,643 (GRCm39)	D233E	probably benign	Het
Srgap3	A	G	6: 112,706,619 (GRCm39)	V826A	probably benign	Het
Tacr2	A	G	10: 62,101,024 (GRCm39)	D378G	probably benign	Het
Taok2	A	G	7: 126,474,347 (GRCm39)	I294T	possibly damaging	Het
Tert	A	G	13: 73,775,528 (GRCm39)	E93G	possibly damaging	Het
Tns2	A	G	15: 102,021,771 (GRCm39)	E1118G	possibly damaging	Het
Topaz1	T	C	9: 122,578,446 (GRCm39)	I452T	probably benign	Het
Trak1	C	T	9: 121,280,800 (GRCm39)		probably benign	Het
Ttc22	A	T	4: 106,480,276 (GRCm39)	I177F	probably damaging	Het
Tuba8	A	G	6: 121,199,697 (GRCm39)	D127G	possibly damaging	Het
Tulp4	A	G	17: 6,248,983 (GRCm39)	M1V	probably null	Het
Urb1	A	G	16: 90,594,791 (GRCm39)	L247P	probably damaging	Het
Usp32	A	G	11: 84,916,362 (GRCm39)	W861R	probably damaging	Het
Vmn1r48	G	A	6: 90,013,360 (GRCm39)	T155I	probably benign	Het
Vmn2r117	A	G	17: 23,679,352 (GRCm39)	L624P	probably damaging	Het
Vmn2r86	T	C	10: 130,291,673 (GRCm39)	R31G	probably damaging	Het
Vstm5	T	G	9: 15,168,594 (GRCm39)	S53A	probably benign	Het
Wnt5a	T	C	14: 28,244,445 (GRCm39)	Y231H	probably benign	Het
Yeats2	T	C	16: 20,012,395 (GRCm39)	V531A	probably damaging	Het
Zw10	T	C	9: 48,988,860 (GRCm39)	Y709H	probably damaging	Het

Other mutations in Cdc14b

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00778:Cdc14b	APN	13	64,363,470 (GRCm39)	missense	probably damaging	1.00
IGL00816:Cdc14b	APN	13	64,353,217 (GRCm39)	missense	probably benign	0.10
IGL02569:Cdc14b	APN	13	64,373,428 (GRCm39)	missense	probably benign	0.36
IGL02634:Cdc14b	APN	13	64,364,117 (GRCm39)	splice site	probably benign
IGL02897:Cdc14b	APN	13	64,395,067 (GRCm39)	missense	probably benign	0.00
R0390:Cdc14b	UTSW	13	64,358,006 (GRCm39)	unclassified	probably benign
R0542:Cdc14b	UTSW	13	64,391,497 (GRCm39)	missense	probably benign	0.01
R1022:Cdc14b	UTSW	13	64,363,490 (GRCm39)	missense	probably damaging	1.00
R1024:Cdc14b	UTSW	13	64,363,490 (GRCm39)	missense	probably damaging	1.00
R1676:Cdc14b	UTSW	13	64,373,416 (GRCm39)	missense	possibly damaging	0.93
R1945:Cdc14b	UTSW	13	64,367,704 (GRCm39)	missense	probably damaging	1.00
R1964:Cdc14b	UTSW	13	64,363,351 (GRCm39)	missense	probably damaging	1.00
R4359:Cdc14b	UTSW	13	64,396,225 (GRCm39)	missense	probably benign	0.27
R4598:Cdc14b	UTSW	13	64,395,088 (GRCm39)	missense	probably benign
R4716:Cdc14b	UTSW	13	64,357,014 (GRCm39)	missense	probably damaging	1.00
R6196:Cdc14b	UTSW	13	64,353,338 (GRCm39)	intron	probably benign
R6219:Cdc14b	UTSW	13	64,353,338 (GRCm39)	intron	probably benign
R6361:Cdc14b	UTSW	13	64,364,023 (GRCm39)	splice site	probably null
R6480:Cdc14b	UTSW	13	64,373,464 (GRCm39)	critical splice acceptor site	probably null
R6565:Cdc14b	UTSW	13	64,373,444 (GRCm39)	missense	probably benign	0.01
R6692:Cdc14b	UTSW	13	64,363,377 (GRCm39)	missense	probably damaging	0.98
R7204:Cdc14b	UTSW	13	64,358,012 (GRCm39)	missense	possibly damaging	0.83
R7327:Cdc14b	UTSW	13	64,373,461 (GRCm39)	missense	probably damaging	1.00
R7464:Cdc14b	UTSW	13	64,344,489 (GRCm39)	nonsense	probably null
R7639:Cdc14b	UTSW	13	64,353,143 (GRCm39)	missense	possibly damaging	0.96
R7687:Cdc14b	UTSW	13	64,357,007 (GRCm39)	missense	probably benign	0.15
R7949:Cdc14b	UTSW	13	64,338,212 (GRCm39)	splice site	probably null
R8170:Cdc14b	UTSW	13	64,363,549 (GRCm39)	splice site	probably null
R9047:Cdc14b	UTSW	13	64,368,758 (GRCm39)	intron	probably benign
Z1176:Cdc14b	UTSW	13	64,422,483 (GRCm39)	missense	possibly damaging	0.66

Predicted Primers

PCR Primer
(F):5'- AGCACACCTGGTCTCAAACG -3'
(R):5'- CTCTCTGCTAATGAGGTGCATAAC -3'

Sequencing Primer
(F):5'- ACACCTGGTCTCAAACGGTTTTG -3'
(R):5'- GAGGTGCATAACTCTAGCTCACTTG -3'

Posted On

2015-03-25