Mutagenetix > Incidental Mutation

Incidental Mutation 'R1066:Vmn2r86'

86014

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r86

Ensembl Gene

ENSMUSG00000092162

Gene Name

vomeronasal 2, receptor 86

Synonyms

EG625109

MMRRC Submission

039152-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R1066 (G1)

Quality Score

206

Status

Not validated

Chromosome

Chromosomal Location

130282068-130291763 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to T at 130282145 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 824 (V824I)

Ref Sequence

ENSEMBL: ENSMUSP00000126596 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170257]

AlphaFold

G5E8Y4

Predicted Effect

probably benign
Transcript: ENSMUST00000170257
AA Change: V824I

PolyPhen 2 Score 0.006 (Sensitivity: 0.97; Specificity: 0.75)

SMART Domains

Protein: ENSMUSP00000126596
Gene: ENSMUSG00000092162
AA Change: V824I

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	1.1e-25	PFAM
Pfam:NCD3G	508	562	2.4e-19	PFAM
Pfam:7tm_3	595	829	6.4e-55	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.1%
20x: 94.1%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 37 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adamts8	T	A	9: 30,867,837 (GRCm39)	C554S	probably damaging	Het
Adarb2	T	G	13: 8,807,359 (GRCm39)	F720C	probably benign	Het
Arid5b	T	C	10: 67,934,186 (GRCm39)	D572G	probably benign	Het
BB014433	C	T	8: 15,092,185 (GRCm39)	V223M	probably damaging	Het
Boc	T	C	16: 44,311,047 (GRCm39)		probably null	Het
Brf2	T	C	8: 27,613,974 (GRCm39)	E404G	probably benign	Het
Ces3a	T	A	8: 105,782,288 (GRCm39)	H380Q	probably benign	Het
Chd9	T	A	8: 91,712,764 (GRCm39)	Y389*	probably null	Het
Csmd3	A	G	15: 47,777,361 (GRCm39)	F1182L	probably damaging	Het
Dnah2	C	A	11: 69,338,645 (GRCm39)	W3169L	probably damaging	Het
Dnah3	T	A	7: 119,660,232 (GRCm39)	E802D	probably damaging	Het
Dtx4	G	T	19: 12,478,373 (GRCm39)	T70K	probably damaging	Het
Fat4	T	C	3: 39,011,376 (GRCm39)	Y2159H	probably damaging	Het
Flrt2	T	A	12: 95,745,833 (GRCm39)	V57E	probably damaging	Het
Gsdmc2	T	A	15: 63,696,899 (GRCm39)	Y424F	possibly damaging	Het
Igfn1	T	C	1: 135,898,463 (GRCm39)	E701G	probably benign	Het
Klhl42	T	C	6: 147,009,397 (GRCm39)	V412A	probably benign	Het
Mkln1	A	C	6: 31,395,922 (GRCm39)	N52T	possibly damaging	Het
Msantd5f6	C	A	4: 73,320,066 (GRCm39)	V238L	possibly damaging	Het
Myo15b	A	T	11: 115,770,577 (GRCm39)	M1519L	probably benign	Het
Nup155	A	T	15: 8,187,244 (GRCm39)	H1391L	probably damaging	Het
Or5b113	T	C	19: 13,342,451 (GRCm39)	M153T	probably benign	Het
P4ha3	G	T	7: 99,967,270 (GRCm39)	V360L	possibly damaging	Het
Pals2	A	T	6: 50,122,847 (GRCm39)	N31I	possibly damaging	Het
Phf14	T	A	6: 11,987,254 (GRCm39)	D611E	possibly damaging	Het
Pik3r1	T	A	13: 101,825,171 (GRCm39)	R465S	probably damaging	Het
Reep3	G	T	10: 66,870,445 (GRCm39)	T117K	probably damaging	Het
Reln	G	T	5: 22,239,662 (GRCm39)	N868K	probably damaging	Het
Sdcbp	T	G	4: 6,385,120 (GRCm39)	I113S	probably damaging	Het
Sema4c	A	T	1: 36,589,281 (GRCm39)	V615E	possibly damaging	Het
Slc25a18	A	G	6: 120,765,249 (GRCm39)		probably null	Het
Tedc2	T	A	17: 24,435,291 (GRCm39)	E366V	probably damaging	Het
Tedc2	C	A	17: 24,435,292 (GRCm39)	E366*	probably null	Het
Uba2	A	T	7: 33,858,247 (GRCm39)	F70I	probably damaging	Het
Usp42	A	T	5: 143,703,796 (GRCm39)	H422Q	probably damaging	Het
Vps50	A	C	6: 3,533,565 (GRCm39)	T266P	probably damaging	Het
Znhit6	T	A	3: 145,284,252 (GRCm39)	D141E	probably damaging	Het

Other mutations in Vmn2r86

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01295:Vmn2r86	APN	10	130,288,895 (GRCm39)	missense	probably damaging	0.99
IGL01328:Vmn2r86	APN	10	130,288,365 (GRCm39)	missense	possibly damaging	0.78
IGL01377:Vmn2r86	APN	10	130,288,855 (GRCm39)	missense	probably damaging	0.99
IGL01548:Vmn2r86	APN	10	130,282,151 (GRCm39)	missense	probably benign	0.22
IGL01804:Vmn2r86	APN	10	130,288,858 (GRCm39)	missense	probably damaging	0.99
IGL01921:Vmn2r86	APN	10	130,291,610 (GRCm39)	missense	probably benign	0.00
IGL02406:Vmn2r86	APN	10	130,284,508 (GRCm39)	missense	possibly damaging	0.81
IGL02625:Vmn2r86	APN	10	130,288,781 (GRCm39)	missense	probably damaging	1.00
IGL02960:Vmn2r86	APN	10	130,289,636 (GRCm39)	missense	possibly damaging	0.74
IGL03104:Vmn2r86	APN	10	130,282,501 (GRCm39)	missense	probably damaging	1.00
R0408:Vmn2r86	UTSW	10	130,282,723 (GRCm39)	missense	probably damaging	1.00
R0437:Vmn2r86	UTSW	10	130,282,412 (GRCm39)	missense	probably damaging	1.00
R0577:Vmn2r86	UTSW	10	130,288,444 (GRCm39)	missense	probably benign	0.04
R0726:Vmn2r86	UTSW	10	130,282,265 (GRCm39)	missense	probably damaging	1.00
R0811:Vmn2r86	UTSW	10	130,289,497 (GRCm39)	missense	probably benign	0.00
R0812:Vmn2r86	UTSW	10	130,289,497 (GRCm39)	missense	probably benign	0.00
R1055:Vmn2r86	UTSW	10	130,282,226 (GRCm39)	missense	probably damaging	1.00
R1199:Vmn2r86	UTSW	10	130,284,443 (GRCm39)	splice site	probably benign
R1332:Vmn2r86	UTSW	10	130,282,739 (GRCm39)	missense	probably damaging	1.00
R1568:Vmn2r86	UTSW	10	130,289,010 (GRCm39)	missense	probably benign	0.09
R1866:Vmn2r86	UTSW	10	130,282,255 (GRCm39)	missense	probably damaging	1.00
R1897:Vmn2r86	UTSW	10	130,288,314 (GRCm39)	missense	probably damaging	1.00
R2017:Vmn2r86	UTSW	10	130,282,582 (GRCm39)	missense	probably benign	0.39
R3162:Vmn2r86	UTSW	10	130,291,673 (GRCm39)	missense	probably damaging	0.99
R3162:Vmn2r86	UTSW	10	130,291,673 (GRCm39)	missense	probably damaging	0.99
R3858:Vmn2r86	UTSW	10	130,291,594 (GRCm39)	missense	probably benign
R4049:Vmn2r86	UTSW	10	130,282,966 (GRCm39)	missense	probably damaging	0.98
R4378:Vmn2r86	UTSW	10	130,288,469 (GRCm39)	missense	possibly damaging	0.67
R4411:Vmn2r86	UTSW	10	130,288,469 (GRCm39)	missense	possibly damaging	0.67
R4413:Vmn2r86	UTSW	10	130,288,469 (GRCm39)	missense	possibly damaging	0.67
R4422:Vmn2r86	UTSW	10	130,288,845 (GRCm39)	missense	possibly damaging	0.87
R4738:Vmn2r86	UTSW	10	130,282,939 (GRCm39)	missense	probably damaging	0.99
R4767:Vmn2r86	UTSW	10	130,291,606 (GRCm39)	missense	probably benign	0.00
R4872:Vmn2r86	UTSW	10	130,289,460 (GRCm39)	missense	probably damaging	0.98
R4880:Vmn2r86	UTSW	10	130,289,484 (GRCm39)	missense	probably benign	0.33
R5092:Vmn2r86	UTSW	10	130,282,456 (GRCm39)	missense	probably damaging	1.00
R5421:Vmn2r86	UTSW	10	130,282,805 (GRCm39)	missense	probably benign	0.41
R6007:Vmn2r86	UTSW	10	130,289,535 (GRCm39)	missense	probably damaging	1.00
R6330:Vmn2r86	UTSW	10	130,282,396 (GRCm39)	missense	probably benign	0.05
R6355:Vmn2r86	UTSW	10	130,291,763 (GRCm39)	start codon destroyed	probably damaging	0.98
R6397:Vmn2r86	UTSW	10	130,282,131 (GRCm39)	nonsense	probably null
R6419:Vmn2r86	UTSW	10	130,282,795 (GRCm39)	missense	probably damaging	1.00
R6933:Vmn2r86	UTSW	10	130,282,126 (GRCm39)	missense	probably damaging	1.00
R6937:Vmn2r86	UTSW	10	130,284,523 (GRCm39)	missense	probably damaging	1.00
R6959:Vmn2r86	UTSW	10	130,282,400 (GRCm39)	missense	probably damaging	1.00
R7010:Vmn2r86	UTSW	10	130,291,726 (GRCm39)	missense	probably benign
R7549:Vmn2r86	UTSW	10	130,282,697 (GRCm39)	missense	probably damaging	1.00
R8179:Vmn2r86	UTSW	10	130,288,953 (GRCm39)	missense	probably benign	0.00
R8257:Vmn2r86	UTSW	10	130,288,279 (GRCm39)	missense	possibly damaging	0.87
R8286:Vmn2r86	UTSW	10	130,285,855 (GRCm39)	missense	probably benign	0.03
R8479:Vmn2r86	UTSW	10	130,282,735 (GRCm39)	missense	probably damaging	1.00
R8805:Vmn2r86	UTSW	10	130,282,396 (GRCm39)	missense	probably benign	0.05
R8960:Vmn2r86	UTSW	10	130,289,672 (GRCm39)	missense	probably benign	0.27
R9021:Vmn2r86	UTSW	10	130,282,934 (GRCm39)	missense	probably damaging	1.00
R9120:Vmn2r86	UTSW	10	130,289,677 (GRCm39)	missense	probably benign	0.00
R9137:Vmn2r86	UTSW	10	130,282,409 (GRCm39)	missense	probably damaging	1.00
R9311:Vmn2r86	UTSW	10	130,288,440 (GRCm39)	missense	probably damaging	1.00
R9312:Vmn2r86	UTSW	10	130,288,406 (GRCm39)	missense	probably benign	0.02
R9433:Vmn2r86	UTSW	10	130,282,567 (GRCm39)	missense	possibly damaging	0.88
R9696:Vmn2r86	UTSW	10	130,285,702 (GRCm39)	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- GGCCCTCAACAATATGCAATGCTGTAA -3'
(R):5'- CTGACAGATTCAATGAAGCCAAGTTCCT -3'

Sequencing Primer
(F):5'- tccatcctatctacagtcttctttc -3'
(R):5'- GAAGCCAAGTTCCTAACTTTCAG -3'

Posted On

2013-11-18