Mutagenetix > Incidental Mutation

Incidental Mutation 'R0577:Vmn2r86'

56267

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r86

Ensembl Gene

ENSMUSG00000092162

Gene Name

vomeronasal 2, receptor 86

Synonyms

EG625109

MMRRC Submission

038767-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.080) question?

Stock #

R0577 (G1)

Quality Score

167

Status

Validated

Chromosome

Chromosomal Location

130282068-130291763 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 130288444 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Arginine to Serine at position 352 (R352S)

Ref Sequence

ENSEMBL: ENSMUSP00000126596 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170257]

AlphaFold

G5E8Y4

Predicted Effect

probably benign
Transcript: ENSMUST00000170257
AA Change: R352S

PolyPhen 2 Score 0.042 (Sensitivity: 0.94; Specificity: 0.83)

SMART Domains

Protein: ENSMUSP00000126596
Gene: ENSMUSG00000092162
AA Change: R352S

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	1.1e-25	PFAM
Pfam:NCD3G	508	562	2.4e-19	PFAM
Pfam:7tm_3	595	829	6.4e-55	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.4%
3x: 98.9%
10x: 97.5%
20x: 95.2%

Validation Efficiency

97% (30/31)

Allele List at MGI

Other mutations in this stock

Total: 30 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aars1	T	A	8: 111,769,910 (GRCm39)	H336Q	probably benign	Het
Abcc2	A	T	19: 43,807,840 (GRCm39)	D827V	probably damaging	Het
Asph	G	T	4: 9,604,620 (GRCm39)	A139E	probably benign	Het
Bag3	T	C	7: 128,125,611 (GRCm39)	M10T	probably benign	Het
Bod1l	T	C	5: 41,952,230 (GRCm39)	D2894G	probably damaging	Het
Cdk12	T	C	11: 98,094,332 (GRCm39)	S47P	probably damaging	Het
Dchs1	C	T	7: 105,413,462 (GRCm39)	V1118I	possibly damaging	Het
Ddi2	A	T	4: 141,411,818 (GRCm39)	C365S	possibly damaging	Het
Eef1g	A	G	19: 8,950,406 (GRCm39)	D264G	probably benign	Het
Fbxw17	T	C	13: 50,585,619 (GRCm39)	L274P	probably benign	Het
Gpc6	A	G	14: 117,673,420 (GRCm39)	T226A	probably benign	Het
Klf12	T	A	14: 100,260,585 (GRCm39)	Y48F	probably damaging	Het
Klhdc4	C	T	8: 122,548,090 (GRCm39)	A67T	probably damaging	Het
Macir	C	T	1: 97,589,551 (GRCm39)		probably null	Het
Madd	C	T	2: 90,968,740 (GRCm39)	E1596K	possibly damaging	Het
Mov10l1	A	G	15: 88,889,930 (GRCm39)	Y533C	probably damaging	Het
Mtif2	G	T	11: 29,490,862 (GRCm39)		probably null	Het
Mtmr6	G	A	14: 60,534,087 (GRCm39)	V442I	possibly damaging	Het
Or4k15b	C	T	14: 50,272,249 (GRCm39)	G204R	probably damaging	Het
Or5ac23	A	T	16: 59,149,061 (GRCm39)	D270E	probably benign	Het
Or9i16	T	C	19: 13,865,167 (GRCm39)	T136A	probably damaging	Het
Pdcd11	A	G	19: 47,087,271 (GRCm39)	N277S	probably benign	Het
Pias2	A	T	18: 77,184,977 (GRCm39)	L12F	probably damaging	Het
Potefam1	G	T	2: 111,024,694 (GRCm39)	Q57K	probably benign	Het
Rnf213	G	T	11: 119,334,106 (GRCm39)	R3105L	probably damaging	Het
Rps11	A	G	7: 44,772,274 (GRCm39)	V111A	probably benign	Het
Rrs1	C	A	1: 9,616,026 (GRCm39)		probably null	Het
Thsd7a	T	C	6: 12,321,047 (GRCm39)	T1543A	possibly damaging	Het
Zfp141	T	C	7: 42,125,938 (GRCm39)	N178S	probably benign	Het
Zfp955a	A	T	17: 33,461,068 (GRCm39)	F355I	probably damaging	Het

Other mutations in Vmn2r86

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01295:Vmn2r86	APN	10	130,288,895 (GRCm39)	missense	probably damaging	0.99
IGL01328:Vmn2r86	APN	10	130,288,365 (GRCm39)	missense	possibly damaging	0.78
IGL01377:Vmn2r86	APN	10	130,288,855 (GRCm39)	missense	probably damaging	0.99
IGL01548:Vmn2r86	APN	10	130,282,151 (GRCm39)	missense	probably benign	0.22
IGL01804:Vmn2r86	APN	10	130,288,858 (GRCm39)	missense	probably damaging	0.99
IGL01921:Vmn2r86	APN	10	130,291,610 (GRCm39)	missense	probably benign	0.00
IGL02406:Vmn2r86	APN	10	130,284,508 (GRCm39)	missense	possibly damaging	0.81
IGL02625:Vmn2r86	APN	10	130,288,781 (GRCm39)	missense	probably damaging	1.00
IGL02960:Vmn2r86	APN	10	130,289,636 (GRCm39)	missense	possibly damaging	0.74
IGL03104:Vmn2r86	APN	10	130,282,501 (GRCm39)	missense	probably damaging	1.00
R0408:Vmn2r86	UTSW	10	130,282,723 (GRCm39)	missense	probably damaging	1.00
R0437:Vmn2r86	UTSW	10	130,282,412 (GRCm39)	missense	probably damaging	1.00
R0726:Vmn2r86	UTSW	10	130,282,265 (GRCm39)	missense	probably damaging	1.00
R0811:Vmn2r86	UTSW	10	130,289,497 (GRCm39)	missense	probably benign	0.00
R0812:Vmn2r86	UTSW	10	130,289,497 (GRCm39)	missense	probably benign	0.00
R1055:Vmn2r86	UTSW	10	130,282,226 (GRCm39)	missense	probably damaging	1.00
R1066:Vmn2r86	UTSW	10	130,282,145 (GRCm39)	missense	probably benign	0.01
R1199:Vmn2r86	UTSW	10	130,284,443 (GRCm39)	splice site	probably benign
R1332:Vmn2r86	UTSW	10	130,282,739 (GRCm39)	missense	probably damaging	1.00
R1568:Vmn2r86	UTSW	10	130,289,010 (GRCm39)	missense	probably benign	0.09
R1866:Vmn2r86	UTSW	10	130,282,255 (GRCm39)	missense	probably damaging	1.00
R1897:Vmn2r86	UTSW	10	130,288,314 (GRCm39)	missense	probably damaging	1.00
R2017:Vmn2r86	UTSW	10	130,282,582 (GRCm39)	missense	probably benign	0.39
R3162:Vmn2r86	UTSW	10	130,291,673 (GRCm39)	missense	probably damaging	0.99
R3162:Vmn2r86	UTSW	10	130,291,673 (GRCm39)	missense	probably damaging	0.99
R3858:Vmn2r86	UTSW	10	130,291,594 (GRCm39)	missense	probably benign
R4049:Vmn2r86	UTSW	10	130,282,966 (GRCm39)	missense	probably damaging	0.98
R4378:Vmn2r86	UTSW	10	130,288,469 (GRCm39)	missense	possibly damaging	0.67
R4411:Vmn2r86	UTSW	10	130,288,469 (GRCm39)	missense	possibly damaging	0.67
R4413:Vmn2r86	UTSW	10	130,288,469 (GRCm39)	missense	possibly damaging	0.67
R4422:Vmn2r86	UTSW	10	130,288,845 (GRCm39)	missense	possibly damaging	0.87
R4738:Vmn2r86	UTSW	10	130,282,939 (GRCm39)	missense	probably damaging	0.99
R4767:Vmn2r86	UTSW	10	130,291,606 (GRCm39)	missense	probably benign	0.00
R4872:Vmn2r86	UTSW	10	130,289,460 (GRCm39)	missense	probably damaging	0.98
R4880:Vmn2r86	UTSW	10	130,289,484 (GRCm39)	missense	probably benign	0.33
R5092:Vmn2r86	UTSW	10	130,282,456 (GRCm39)	missense	probably damaging	1.00
R5421:Vmn2r86	UTSW	10	130,282,805 (GRCm39)	missense	probably benign	0.41
R6007:Vmn2r86	UTSW	10	130,289,535 (GRCm39)	missense	probably damaging	1.00
R6330:Vmn2r86	UTSW	10	130,282,396 (GRCm39)	missense	probably benign	0.05
R6355:Vmn2r86	UTSW	10	130,291,763 (GRCm39)	start codon destroyed	probably damaging	0.98
R6397:Vmn2r86	UTSW	10	130,282,131 (GRCm39)	nonsense	probably null
R6419:Vmn2r86	UTSW	10	130,282,795 (GRCm39)	missense	probably damaging	1.00
R6933:Vmn2r86	UTSW	10	130,282,126 (GRCm39)	missense	probably damaging	1.00
R6937:Vmn2r86	UTSW	10	130,284,523 (GRCm39)	missense	probably damaging	1.00
R6959:Vmn2r86	UTSW	10	130,282,400 (GRCm39)	missense	probably damaging	1.00
R7010:Vmn2r86	UTSW	10	130,291,726 (GRCm39)	missense	probably benign
R7549:Vmn2r86	UTSW	10	130,282,697 (GRCm39)	missense	probably damaging	1.00
R8179:Vmn2r86	UTSW	10	130,288,953 (GRCm39)	missense	probably benign	0.00
R8257:Vmn2r86	UTSW	10	130,288,279 (GRCm39)	missense	possibly damaging	0.87
R8286:Vmn2r86	UTSW	10	130,285,855 (GRCm39)	missense	probably benign	0.03
R8479:Vmn2r86	UTSW	10	130,282,735 (GRCm39)	missense	probably damaging	1.00
R8805:Vmn2r86	UTSW	10	130,282,396 (GRCm39)	missense	probably benign	0.05
R8960:Vmn2r86	UTSW	10	130,289,672 (GRCm39)	missense	probably benign	0.27
R9021:Vmn2r86	UTSW	10	130,282,934 (GRCm39)	missense	probably damaging	1.00
R9120:Vmn2r86	UTSW	10	130,289,677 (GRCm39)	missense	probably benign	0.00
R9137:Vmn2r86	UTSW	10	130,282,409 (GRCm39)	missense	probably damaging	1.00
R9311:Vmn2r86	UTSW	10	130,288,440 (GRCm39)	missense	probably damaging	1.00
R9312:Vmn2r86	UTSW	10	130,288,406 (GRCm39)	missense	probably benign	0.02
R9433:Vmn2r86	UTSW	10	130,282,567 (GRCm39)	missense	possibly damaging	0.88
R9696:Vmn2r86	UTSW	10	130,285,702 (GRCm39)	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- TGTGGGCCACAGCATAAACAGC -3'
(R):5'- TGACCGTTTTCCCAATGTCCATCAG -3'

Sequencing Primer
(F):5'- GGCCACAGCATAAACAGCATTATAC -3'
(R):5'- ATGACCAGGGTATTCAGCTTC -3'

Posted On

2013-07-11