Mutagenetix > Incidental Mutation

Incidental Mutation 'R7123:Htr1d'

552135

Institutional Source

Beutler Lab

Gene Symbol

Htr1d

Ensembl Gene

ENSMUSG00000070687

Gene Name

5-hydroxytryptamine (serotonin) receptor 1D

Synonyms

Htr1db, Gpcr14

MMRRC Submission

045211-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R7123 (G1)

Quality Score

224.009

Status

Validated

Chromosome

Chromosomal Location

136150835-136171709 bp(+) (GRCm39)

Type of Mutation

start gained

DNA Base Change (assembly)

C to T at 136169664 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000112402 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000088677] [ENSMUST00000117699] [ENSMUST00000121571]

AlphaFold

Q61224

Predicted Effect

probably benign
Transcript: ENSMUST00000088677

SMART Domains

Protein: ENSMUSP00000086052
Gene: ENSMUSG00000070687

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	46	368	1.2e-11	PFAM
Pfam:7tm_1	52	353	1.2e-75	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000117699

SMART Domains

Protein: ENSMUSP00000112840
Gene: ENSMUSG00000070687

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	46	368	1.2e-11	PFAM
Pfam:7tm_1	52	353	2.7e-86	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000121571

SMART Domains

Protein: ENSMUSP00000112402
Gene: ENSMUSG00000070687

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srsx	46	368	1.2e-11	PFAM
Pfam:7tm_1	52	353	2.7e-86	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 100.0%
3x: 100.0%
10x: 99.7%
20x: 99.1%

Validation Efficiency

98% (52/53)

MGI Phenotype

PHENOTYPE: Mice homozygous for disruptions in this gene display a normal phenotype. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca17	A	G	17: 24,484,949 (GRCm39)	I1521T	probably damaging	Het
Adgrv1	A	T	13: 81,740,693 (GRCm39)	I145N	probably damaging	Het
Ago3	C	A	4: 126,248,798 (GRCm39)		probably null	Het
Agrn	A	T	4: 156,257,297 (GRCm39)	W1178R	probably benign	Het
Akap3	A	T	6: 126,843,267 (GRCm39)	I629L	probably benign	Het
Aknad1	T	G	3: 108,682,560 (GRCm39)	Y545*	probably null	Het
Anapc1	C	A	2: 128,454,930 (GRCm39)	V1925F	probably damaging	Het
Barhl1	C	T	2: 28,799,943 (GRCm39)		probably null	Het
Cfap157	T	C	2: 32,669,413 (GRCm39)	E294G	probably damaging	Het
Cfd	G	A	10: 79,728,331 (GRCm39)	G231S	probably damaging	Het
Csf2ra	A	G	19: 61,215,300 (GRCm39)	V105A	probably damaging	Het
Dhtkd1	A	T	2: 5,922,591 (GRCm39)	W523R	probably damaging	Het
Disp1	C	A	1: 182,869,030 (GRCm39)	R1130L	probably damaging	Het
Dnajc22	A	C	15: 98,999,085 (GRCm39)	Y90S	possibly damaging	Het
Ebna1bp2	A	G	4: 118,482,772 (GRCm39)	K254E	probably damaging	Het
Gfpt1	A	T	6: 87,033,168 (GRCm39)	D131V	probably damaging	Het
Gm5460	A	G	14: 33,763,982 (GRCm39)	I21V	unknown	Het
H2-T9	A	G	17: 36,438,686 (GRCm39)	I235T	possibly damaging	Het
Kndc1	G	A	7: 139,516,749 (GRCm39)	E1570K	probably damaging	Het
Kntc1	T	C	5: 123,919,789 (GRCm39)	Y887H	probably damaging	Het
Kti12	T	C	4: 108,705,679 (GRCm39)	S198P	probably benign	Het
Mcmdc2	A	T	1: 10,010,643 (GRCm39)	I604F	unknown	Het
Mmp8	A	T	9: 7,563,196 (GRCm39)	D253V	probably damaging	Het
Muc4	T	C	16: 32,569,509 (GRCm39)	S252P	possibly damaging	Het
Myo5c	A	G	9: 75,196,505 (GRCm39)	K1317R	probably benign	Het
Ncoa7	T	C	10: 30,530,435 (GRCm39)	I749V	probably benign	Het
Nfrkb	A	G	9: 31,325,311 (GRCm39)		probably null	Het
Obscn	T	C	11: 58,904,477 (GRCm39)	T7166A	probably benign	Het
Obsl1	A	G	1: 75,466,313 (GRCm39)	S1472P	probably damaging	Het
Or14j7	T	A	17: 38,234,567 (GRCm39)	L37M	probably benign	Het
Or1e20-ps1	T	A	11: 73,324,536 (GRCm39)	D172V	unknown	Het
Orc1	A	C	4: 108,445,884 (GRCm39)	M1L	probably damaging	Het
Pkd1	T	A	17: 24,813,742 (GRCm39)	S4069T	possibly damaging	Het
Pus1	T	C	5: 110,921,798 (GRCm39)	*442W	probably null	Het
Rgma	A	C	7: 73,059,139 (GRCm39)	D97A	probably damaging	Het
Ryr1	T	C	7: 28,746,279 (GRCm39)	K3834E	probably benign	Het
Sars2	T	C	7: 28,452,866 (GRCm39)	V475A	probably benign	Het
Scamp2	A	T	9: 57,494,385 (GRCm39)	T253S	probably benign	Het
Skint9	C	T	4: 112,248,174 (GRCm39)	W190*	probably null	Het
Spata31d1e	G	A	13: 59,891,254 (GRCm39)	Q189*	probably null	Het
Sv2b	C	T	7: 74,767,450 (GRCm39)	V649I	possibly damaging	Het
Synpo2	A	G	3: 122,906,835 (GRCm39)	V827A	probably benign	Het
Tiparp	A	T	3: 65,460,948 (GRCm39)	I646F	probably damaging	Het
Topaz1	G	A	9: 122,577,480 (GRCm39)	G130D	probably damaging	Het
Trio	TACCTTGTTACTGAGCCCTTCTCACCTTCACAGACACCTTGTTACTGAGCCCTTCTCACCTTCACAGATACCTTGTTACTGAGCCCTTCTC	TACCTTGTTACTGAGCCCTTCTCACCTTCACAGATACCTTGTTACTGAGCCCTTCTC	15: 27,742,399 (GRCm39)		probably benign	Het
Ttll7	C	T	3: 146,619,051 (GRCm39)	P319S	possibly damaging	Het
Umodl1	T	A	17: 31,201,318 (GRCm39)	F416I	possibly damaging	Het
Vmn2r104	T	C	17: 20,261,088 (GRCm39)	D445G	probably benign	Het
Wnk2	A	G	13: 49,235,462 (GRCm39)	V651A	possibly damaging	Het
Zbtb21	C	T	16: 97,751,112 (GRCm39)	S1057N	probably damaging	Het
Zfp451	A	T	1: 33,815,950 (GRCm39)	C667S	probably damaging	Het

Other mutations in Htr1d

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01375:Htr1d	APN	4	136,170,484 (GRCm39)	missense	probably benign	0.03
IGL01818:Htr1d	APN	4	136,170,197 (GRCm39)	missense	probably benign	0.02
IGL01952:Htr1d	APN	4	136,170,872 (GRCm39)	missense	probably benign	0.08
IGL02696:Htr1d	APN	4	136,170,722 (GRCm39)	missense	probably benign	0.00
R0112:Htr1d	UTSW	4	136,170,311 (GRCm39)	missense	probably benign	0.05
R0147:Htr1d	UTSW	4	136,170,788 (GRCm39)	missense	probably damaging	1.00
R0148:Htr1d	UTSW	4	136,170,788 (GRCm39)	missense	probably damaging	1.00
R2483:Htr1d	UTSW	4	136,170,815 (GRCm39)	missense	probably damaging	0.97
R2764:Htr1d	UTSW	4	136,170,376 (GRCm39)	missense	possibly damaging	0.89
R3622:Htr1d	UTSW	4	136,170,815 (GRCm39)	missense	probably damaging	0.97
R3623:Htr1d	UTSW	4	136,170,815 (GRCm39)	missense	probably damaging	0.97
R3624:Htr1d	UTSW	4	136,170,815 (GRCm39)	missense	probably damaging	0.97
R3894:Htr1d	UTSW	4	136,170,548 (GRCm39)	missense	probably benign	0.00
R4567:Htr1d	UTSW	4	136,170,836 (GRCm39)	missense	probably benign	0.17
R4735:Htr1d	UTSW	4	136,170,197 (GRCm39)	missense	probably benign	0.02
R6190:Htr1d	UTSW	4	136,170,109 (GRCm39)	missense	probably damaging	1.00
R7011:Htr1d	UTSW	4	136,170,317 (GRCm39)	missense	probably benign	0.10
R7223:Htr1d	UTSW	4	136,170,812 (GRCm39)	missense	probably damaging	1.00
R7328:Htr1d	UTSW	4	136,170,614 (GRCm39)	missense	probably benign	0.01
R8399:Htr1d	UTSW	4	136,170,686 (GRCm39)	missense	probably damaging	1.00
R8514:Htr1d	UTSW	4	136,170,650 (GRCm39)	missense	probably damaging	1.00
R8829:Htr1d	UTSW	4	136,170,554 (GRCm39)	missense	probably benign
R8982:Htr1d	UTSW	4	136,170,866 (GRCm39)	missense	possibly damaging	0.89
R9497:Htr1d	UTSW	4	136,169,663 (GRCm39)	start gained	probably benign
R9505:Htr1d	UTSW	4	136,170,889 (GRCm39)	missense	probably benign

Predicted Primers

PCR Primer
(F):5'- CATCCTCCTGTGAGACCTTG -3'
(R):5'- ACATTCAGGGATCTGTTGGAG -3'

Sequencing Primer
(F):5'- CAAACTTATCATGTGTCCACTGGG -3'
(R):5'- CAGGGATCTGTTGGAGGCCTC -3'

Posted On

2019-05-15