Incidental Mutation 'R6469:Rpl3'
ID 540728
Institutional Source Beutler Lab
Gene Symbol Rpl3
Ensembl Gene ENSMUSG00000060036
Gene Name ribosomal protein L3
Synonyms J1, F2
MMRRC Submission 044602-MU
Accession Numbers
Essential gene? Probably essential (E-score: 0.942) question?
Stock # R6469 (G1)
Quality Score 145.008
Status Validated
Chromosome 15
Chromosomal Location 79961993-79967553 bp(-) (GRCm39)
Type of Mutation splice site
DNA Base Change (assembly) A to T at 79967546 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000080354 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000081650]
AlphaFold P27659
Predicted Effect probably null
Transcript: ENSMUST00000081650
SMART Domains Protein: ENSMUSP00000080354
Gene: ENSMUSG00000060036

DomainStartEndE-ValueType
Pfam:Ribosomal_L3 1 375 1.7e-194 PFAM
Meta Mutation Damage Score 0.9755 question?
Coding Region Coverage
  • 1x: 99.9%
  • 3x: 99.4%
  • 10x: 97.2%
  • 20x: 91.3%
Validation Efficiency 100% (43/43)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Ribosomes, the complexes that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L3P family of ribosomal proteins and it is located in the cytoplasm. The protein can bind to the HIV-1 TAR mRNA, and it has been suggested that the protein contributes to tat-mediated transactivation. This gene is co-transcribed with several small nucleolar RNA genes, which are located in several of this gene's introns. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008]
Allele List at MGI
Other mutations in this stock
Total: 41 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1110002E22Rik T G 3: 137,772,736 (GRCm39) S642A probably damaging Het
Aadacl2 A T 3: 59,932,210 (GRCm39) T242S probably benign Het
Adprh T C 16: 38,270,671 (GRCm39) M45V probably benign Het
Akr1c13 T C 13: 4,246,511 (GRCm39) probably null Het
Ap3d1 A C 10: 80,547,992 (GRCm39) V900G probably benign Het
Bak1 G A 17: 27,240,293 (GRCm39) R125C probably damaging Het
Bmpr1b T C 3: 141,562,222 (GRCm39) T322A possibly damaging Het
Camsap3 T A 8: 3,653,941 (GRCm39) L521Q possibly damaging Het
Col6a6 A T 9: 105,575,890 (GRCm39) F2157I probably damaging Het
Dcun1d4 G A 5: 73,691,957 (GRCm39) M155I probably damaging Het
Diaph3 T C 14: 86,893,974 (GRCm39) S12G possibly damaging Het
Enpp5 G A 17: 44,396,155 (GRCm39) G356S probably damaging Het
Fan1 T A 7: 64,022,234 (GRCm39) N340Y probably damaging Het
Fgb A T 3: 82,953,449 (GRCm39) L107* probably null Het
Ganab T C 19: 8,879,996 (GRCm39) probably null Het
Gba1 C T 3: 89,111,388 (GRCm39) P51L probably benign Het
Glb1l2 T C 9: 26,707,828 (GRCm39) D60G probably benign Het
Idh3b AG AGCACCACAACTG 2: 130,121,593 (GRCm39) probably null Het
Itih2 A G 2: 10,128,224 (GRCm39) V159A possibly damaging Het
Kif1b T C 4: 149,277,053 (GRCm39) M1337V probably benign Het
Lrch1 C T 14: 75,054,525 (GRCm39) R323Q probably damaging Het
Lrrc30 T C 17: 67,938,860 (GRCm39) N240S probably benign Het
Mrgpra9 T C 7: 46,884,854 (GRCm39) Y271C probably benign Het
Ncor1 T C 11: 62,234,128 (GRCm39) H682R probably damaging Het
Or14j4 A T 17: 37,921,204 (GRCm39) V146E probably damaging Het
Pax9 C A 12: 56,743,648 (GRCm39) F98L probably damaging Het
Phlpp1 G A 1: 106,214,833 (GRCm39) R585Q probably damaging Het
Prkdc A G 16: 15,612,939 (GRCm39) T3166A probably benign Het
Prr11 T A 11: 86,988,003 (GRCm39) Q300L possibly damaging Het
Rad50 T A 11: 53,575,062 (GRCm39) E620D probably benign Het
Sacs G T 14: 61,428,697 (GRCm39) G252V probably damaging Het
Serpina3i T A 12: 104,232,776 (GRCm39) V227E probably damaging Het
Smap2 GACTCTAC GAC 4: 120,830,282 (GRCm39) probably benign Het
Snx19 T C 9: 30,339,039 (GRCm39) V59A possibly damaging Het
St3gal1 A G 15: 66,983,195 (GRCm39) V187A possibly damaging Het
Tcstv2c T A 13: 120,616,349 (GRCm39) W63R probably damaging Het
Tsc1 G A 2: 28,561,898 (GRCm39) probably null Het
Vmn2r61 C T 7: 41,915,283 (GRCm39) Q77* probably null Het
Wdr72 A G 9: 74,120,643 (GRCm39) H954R probably benign Het
Zbtb21 T C 16: 97,757,972 (GRCm39) M20V probably benign Het
Zkscan16 A T 4: 58,956,483 (GRCm39) D255V probably damaging Het
Other mutations in Rpl3
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01790:Rpl3 APN 15 79,964,061 (GRCm39) splice site probably benign
IGL03279:Rpl3 APN 15 79,962,560 (GRCm39) missense probably benign 0.08
R1828:Rpl3 UTSW 15 79,964,591 (GRCm39) missense possibly damaging 0.47
R6368:Rpl3 UTSW 15 79,966,745 (GRCm39) missense probably damaging 1.00
R7657:Rpl3 UTSW 15 79,965,258 (GRCm39) missense probably benign
R8748:Rpl3 UTSW 15 79,963,902 (GRCm39) missense probably benign
Predicted Primers PCR Primer
(F):5'- TTAAGGTCCCTGAATTTCCGG -3'
(R):5'- ATTCTAAACCTCAGCGTCGAC -3'

Sequencing Primer
(F):5'- TGAATTTCCGGACGGTCGC -3'
(R):5'- GTTTCGCTAAGCTTGAACCCAATC -3'
Posted On 2018-11-19