Mutagenetix > Incidental Mutation

Incidental Mutation 'R6920:Kcnma1'

539602

Institutional Source

Beutler Lab

Gene Symbol

Kcnma1

Ensembl Gene

ENSMUSG00000063142

Gene Name

potassium large conductance calcium-activated channel, subfamily M, alpha member 1

Synonyms

MaxiK, BKCa, 5730414M22Rik, BK channel alpha subunit, Slo, Slo1, mSlo1

MMRRC Submission

045040-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.837) question?

Stock #

R6920 (G1)

Quality Score

200.009

Status

Validated

Chromosome

Chromosomal Location

23342356-24055173 bp(-) (GRCm39)

Type of Mutation

critical splice donor site (2 bp from exon)

DNA Base Change (assembly)

A to G at 23576602 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000153316 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000065788] [ENSMUST00000074983] [ENSMUST00000100831] [ENSMUST00000112423] [ENSMUST00000145596] [ENSMUST00000163322] [ENSMUST00000172099] [ENSMUST00000177634] [ENSMUST00000179097] [ENSMUST00000179836] [ENSMUST00000188210] [ENSMUST00000188285] [ENSMUST00000188991] [ENSMUST00000190044] [ENSMUST00000190985] [ENSMUST00000212576] [ENSMUST00000223655] [ENSMUST00000223727] [ENSMUST00000223749] [ENSMUST00000224077] [ENSMUST00000224232] [ENSMUST00000224285] [ENSMUST00000224468] [ENSMUST00000224787] [ENSMUST00000224812] [ENSMUST00000225315] [ENSMUST00000225431] [ENSMUST00000225471] [ENSMUST00000225556] [ENSMUST00000225794] [ENSMUST00000226051]

AlphaFold

no structure available at present

Predicted Effect

probably benign
Transcript: ENSMUST00000065788

SMART Domains

Protein: ENSMUSP00000065293
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
low complexity region	20	31	N/A	INTRINSIC
transmembrane domain	54	73	N/A	INTRINSIC
Pfam:Ion_trans	91	262	2.2e-18	PFAM
Pfam:Ion_trans_2	180	268	5.7e-16	PFAM
Pfam:TrkA_N	314	413	7.5e-7	PFAM
Pfam:BK_channel_a	411	509	6.4e-31	PFAM
low complexity region	835	843	N/A	INTRINSIC
low complexity region	891	902	N/A	INTRINSIC
low complexity region	1005	1031	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000074983

SMART Domains

Protein: ENSMUSP00000074511
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
low complexity region	20	31	N/A	INTRINSIC
transmembrane domain	54	73	N/A	INTRINSIC
Pfam:Ion_trans	91	262	2.2e-18	PFAM
Pfam:Ion_trans_2	180	268	5.7e-16	PFAM
Pfam:TrkA_N	314	413	7.5e-7	PFAM
Pfam:BK_channel_a	411	509	6.4e-31	PFAM
low complexity region	894	902	N/A	INTRINSIC
low complexity region	950	961	N/A	INTRINSIC
low complexity region	1064	1090	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000100831

SMART Domains

Protein: ENSMUSP00000098393
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
low complexity region	20	31	N/A	INTRINSIC
transmembrane domain	54	73	N/A	INTRINSIC
Pfam:Ion_trans	91	262	2.1e-18	PFAM
Pfam:Ion_trans_2	180	268	5.5e-16	PFAM
Pfam:TrkA_N	314	413	7.3e-7	PFAM
Pfam:BK_channel_a	411	509	6.2e-31	PFAM
low complexity region	865	873	N/A	INTRINSIC
low complexity region	921	932	N/A	INTRINSIC
low complexity region	1035	1061	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000112423

SMART Domains

Protein: ENSMUSP00000108042
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
transmembrane domain	2	19	N/A	INTRINSIC
Pfam:Ion_trans	37	208	2.1e-18	PFAM
Pfam:Ion_trans_2	126	214	5.3e-16	PFAM
Pfam:TrkA_N	260	359	7e-7	PFAM
Pfam:BK_channel_a	357	455	6e-31	PFAM
low complexity region	781	789	N/A	INTRINSIC
low complexity region	837	848	N/A	INTRINSIC
low complexity region	951	977	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000145596

Predicted Effect

probably null
Transcript: ENSMUST00000163322

SMART Domains

Protein: ENSMUSP00000128553
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
low complexity region	20	31	N/A	INTRINSIC
transmembrane domain	54	73	N/A	INTRINSIC
Pfam:Ion_trans	91	262	3.2e-18	PFAM
Pfam:Ion_trans_2	180	268	1.1e-15	PFAM
Pfam:TrkA_N	314	413	7e-7	PFAM
Pfam:BK_channel_a	411	509	6e-31	PFAM
low complexity region	832	840	N/A	INTRINSIC
low complexity region	888	899	N/A	INTRINSIC
low complexity region	1002	1028	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000172099

SMART Domains

Protein: ENSMUSP00000132204
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
low complexity region	20	31	N/A	INTRINSIC
transmembrane domain	54	73	N/A	INTRINSIC
Pfam:Ion_trans	91	262	2.2e-18	PFAM
Pfam:Ion_trans_2	180	268	5.7e-16	PFAM
Pfam:TrkA_N	314	413	7.6e-7	PFAM
Pfam:BK_channel_a	411	509	6.5e-31	PFAM
low complexity region	897	905	N/A	INTRINSIC
low complexity region	953	964	N/A	INTRINSIC
low complexity region	1067	1093	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000177634

SMART Domains

Protein: ENSMUSP00000136447
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
low complexity region	20	31	N/A	INTRINSIC
Pfam:Ion_trans	53	272	4.9e-19	PFAM
Pfam:Ion_trans_2	180	267	1.2e-15	PFAM
Pfam:BK_channel_a	413	508	1.2e-35	PFAM
low complexity region	862	870	N/A	INTRINSIC
low complexity region	918	929	N/A	INTRINSIC
low complexity region	1032	1058	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000179097

SMART Domains

Protein: ENSMUSP00000136568
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
low complexity region	20	31	N/A	INTRINSIC
transmembrane domain	54	73	N/A	INTRINSIC
Pfam:Ion_trans	91	262	4.6e-18	PFAM
Pfam:Ion_trans_2	180	268	1e-15	PFAM
Pfam:TrkA_N	314	413	1.1e-7	PFAM
Pfam:BK_channel_a	411	509	3.2e-31	PFAM
low complexity region	859	867	N/A	INTRINSIC
low complexity region	915	926	N/A	INTRINSIC
low complexity region	1029	1055	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000179836

SMART Domains

Protein: ENSMUSP00000137141
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
low complexity region	20	31	N/A	INTRINSIC
transmembrane domain	54	73	N/A	INTRINSIC
Pfam:Ion_trans	91	262	4.2e-18	PFAM
Pfam:Ion_trans_2	180	268	9.5e-16	PFAM
Pfam:BK_channel_a	389	457	2.4e-15	PFAM
low complexity region	838	846	N/A	INTRINSIC
low complexity region	894	905	N/A	INTRINSIC
low complexity region	1008	1034	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000188210

SMART Domains

Protein: ENSMUSP00000141069
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
low complexity region	4	23	N/A	INTRINSIC
transmembrane domain	86	108	N/A	INTRINSIC
low complexity region	145	156	N/A	INTRINSIC
transmembrane domain	179	198	N/A	INTRINSIC
Pfam:Ion_trans	216	387	1.3e-18	PFAM
Pfam:Ion_trans_2	305	393	5.2e-16	PFAM
Pfam:TrkA_N	439	538	7.8e-7	PFAM
Pfam:BK_channel_a	536	634	5e-31	PFAM
low complexity region	988	996	N/A	INTRINSIC
low complexity region	1044	1055	N/A	INTRINSIC
low complexity region	1158	1184	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000188285

SMART Domains

Protein: ENSMUSP00000140275
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
low complexity region	4	23	N/A	INTRINSIC
transmembrane domain	86	108	N/A	INTRINSIC
low complexity region	145	156	N/A	INTRINSIC
transmembrane domain	179	198	N/A	INTRINSIC
Pfam:Ion_trans	216	387	1.3e-18	PFAM
Pfam:Ion_trans_2	305	393	5.4e-16	PFAM
Pfam:TrkA_N	439	538	8e-7	PFAM
Pfam:BK_channel_a	536	634	5.2e-31	PFAM
low complexity region	1019	1027	N/A	INTRINSIC
low complexity region	1075	1086	N/A	INTRINSIC
low complexity region	1189	1215	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000188991

SMART Domains

Protein: ENSMUSP00000140751
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
low complexity region	4	23	N/A	INTRINSIC
transmembrane domain	86	108	N/A	INTRINSIC
low complexity region	145	156	N/A	INTRINSIC
transmembrane domain	179	198	N/A	INTRINSIC
Pfam:Ion_trans	216	387	3.3e-18	PFAM
Pfam:Ion_trans_2	305	393	1.1e-15	PFAM
Pfam:TrkA_N	439	538	3.7e-7	PFAM
Pfam:BK_channel_a	536	634	3.4e-31	PFAM
low complexity region	1015	1023	N/A	INTRINSIC
low complexity region	1071	1082	N/A	INTRINSIC
low complexity region	1185	1211	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000190044

SMART Domains

Protein: ENSMUSP00000140033
Gene: ENSMUSG00000063142

Domain	Start	End	E-Value	Type
low complexity region	4	23	N/A	INTRINSIC
transmembrane domain	86	108	N/A	INTRINSIC
low complexity region	145	156	N/A	INTRINSIC
transmembrane domain	179	198	N/A	INTRINSIC
Pfam:Ion_trans	216	387	1.3e-18	PFAM
Pfam:Ion_trans_2	305	393	5.1e-16	PFAM
Pfam:TrkA_N	439	538	7.5e-7	PFAM
Pfam:BK_channel_a	536	634	4.9e-31	PFAM
low complexity region	957	965	N/A	INTRINSIC
low complexity region	1013	1024	N/A	INTRINSIC
low complexity region	1127	1153	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000190985

Predicted Effect

probably benign
Transcript: ENSMUST00000212576

Predicted Effect

probably benign
Transcript: ENSMUST00000223655

Predicted Effect

probably benign
Transcript: ENSMUST00000223727

Predicted Effect

probably benign
Transcript: ENSMUST00000223749

Predicted Effect

probably benign
Transcript: ENSMUST00000224025

Predicted Effect

probably benign
Transcript: ENSMUST00000224077

Predicted Effect

probably benign
Transcript: ENSMUST00000224232

Predicted Effect

probably null
Transcript: ENSMUST00000224285

Predicted Effect

probably benign
Transcript: ENSMUST00000224468

Predicted Effect

probably benign
Transcript: ENSMUST00000224787

Predicted Effect

probably benign
Transcript: ENSMUST00000224812

Predicted Effect

probably benign
Transcript: ENSMUST00000225315

Predicted Effect

probably benign
Transcript: ENSMUST00000225431

Predicted Effect

probably benign
Transcript: ENSMUST00000225471

Predicted Effect

probably benign
Transcript: ENSMUST00000225556

Predicted Effect

probably benign
Transcript: ENSMUST00000225794

Predicted Effect

probably benign
Transcript: ENSMUST00000226051

Coding Region Coverage

1x: 100.0%
3x: 99.9%
10x: 99.1%
20x: 97.2%

Validation Efficiency

100% (65/65)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit, which is the product of this gene, and the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
PHENOTYPE: Homozygous inactivation of this gene leads to cerebellar ataxia, Purkinje cell dysfunction, uneven gait patterns, bladder hyperactivity, urinary incontinence, abnormal colonic K+ secretion, and hearing impairment. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 65 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	T	3: 137,773,811 (GRCm39)	Y1000F	probably damaging	Het
1700018B08Rik	A	G	8: 122,262,160 (GRCm39)		probably null	Het
Aadat	T	C	8: 60,982,467 (GRCm39)	F245L	probably damaging	Het
Adcy10	T	G	1: 165,403,227 (GRCm39)	L1575W	probably damaging	Het
Anks4b	A	T	7: 119,782,231 (GRCm39)	T421S	probably damaging	Het
Anpep	A	T	7: 79,475,097 (GRCm39)	I155N	probably damaging	Het
Arnt	T	C	3: 95,397,932 (GRCm39)	F572L	probably damaging	Het
Brip1	G	A	11: 86,039,362 (GRCm39)	Q391*	probably null	Het
Brpf3	C	A	17: 29,042,970 (GRCm39)	H1004N	probably benign	Het
Cand2	T	A	6: 115,768,250 (GRCm39)	V465D	possibly damaging	Het
Card10	G	T	15: 78,686,609 (GRCm39)	Y69*	probably null	Het
Catsperd	A	T	17: 56,962,175 (GRCm39)	K450*	probably null	Het
Ccdc137	T	C	11: 120,351,009 (GRCm39)	L137P	probably damaging	Het
Cic	T	C	7: 24,990,107 (GRCm39)	S1905P	probably damaging	Het
Csmd3	C	T	15: 47,507,601 (GRCm39)	G2971S	probably damaging	Het
Dmxl2	A	G	9: 54,379,496 (GRCm39)	Y183H	probably damaging	Het
Drosha	T	C	15: 12,834,396 (GRCm39)	Y167H	unknown	Het
E330034G19Rik	A	G	14: 24,358,310 (GRCm39)	K214R	unknown	Het
Fam180a	T	G	6: 35,290,765 (GRCm39)	I73L	possibly damaging	Het
Fbxo28	G	T	1: 182,168,986 (GRCm39)	H51N	probably benign	Het
Gm12728	T	G	4: 105,647,533 (GRCm39)		probably null	Het
Gm973	T	C	1: 59,591,620 (GRCm39)	C335R	possibly damaging	Het
Gpx8	A	G	13: 113,179,770 (GRCm39)	V177A	probably damaging	Het
Hdlbp	A	G	1: 93,340,083 (GRCm39)		probably null	Het
Htt	T	G	5: 35,034,444 (GRCm39)	Y1972D	probably null	Het
Igkv6-14	T	C	6: 70,412,116 (GRCm39)	Y56C	possibly damaging	Het
Klc1	T	C	12: 111,754,019 (GRCm39)	S105P	probably damaging	Het
Klhl20	T	C	1: 160,921,266 (GRCm39)	D63G	possibly damaging	Het
Lamc3	A	G	2: 31,798,701 (GRCm39)	D469G	probably damaging	Het
Lrit1	G	C	14: 36,782,052 (GRCm39)	V242L	probably damaging	Het
Mboat4	G	A	8: 34,591,865 (GRCm39)	R434H	probably benign	Het
Mttp	T	C	3: 137,821,043 (GRCm39)	K270E	possibly damaging	Het
Muc5ac	G	A	7: 141,347,035 (GRCm39)	C337Y	possibly damaging	Het
Nars1	A	G	18: 64,634,471 (GRCm39)	V484A	probably damaging	Het
Noxa1	G	T	2: 24,981,844 (GRCm39)		probably null	Het
Or14c45	A	G	7: 86,176,522 (GRCm39)	T186A	probably benign	Het
Or7g18	T	A	9: 18,786,821 (GRCm39)	L63H	probably damaging	Het
Osbpl7	G	A	11: 96,941,584 (GRCm39)	G36S	probably damaging	Het
P4htm	C	T	9: 108,460,812 (GRCm39)	G220D	probably benign	Het
Pcdhga7	A	G	18: 37,848,199 (GRCm39)	I69V	probably benign	Het
Pla2g4e	C	T	2: 120,015,795 (GRCm39)	E250K	possibly damaging	Het
Plcd4	A	G	1: 74,604,994 (GRCm39)		probably benign	Het
Ppfia3	C	A	7: 45,008,231 (GRCm39)	G213V	possibly damaging	Het
Ppp1r1a	A	G	15: 103,441,513 (GRCm39)	S67P	probably damaging	Het
Prss43	T	C	9: 110,657,680 (GRCm39)	F193S	probably benign	Het
Rfx1	A	G	8: 84,822,117 (GRCm39)	Y872C	probably damaging	Het
Rhot1	T	A	11: 80,132,921 (GRCm39)	N218K	probably benign	Het
Sall1	A	G	8: 89,757,021 (GRCm39)	F1028L	probably damaging	Het
Siglec1	G	A	2: 130,919,997 (GRCm39)	Q845*	probably null	Het
Slc38a9	C	T	13: 112,838,060 (GRCm39)	T275I	possibly damaging	Het
Slc39a4	A	T	15: 76,497,470 (GRCm39)	S481T	probably damaging	Het
Ssr1	A	T	13: 38,169,998 (GRCm39)	N191K	probably damaging	Het
Tenm4	A	T	7: 96,544,757 (GRCm39)	S2258C	probably damaging	Het
Tm7sf3	T	G	6: 146,507,645 (GRCm39)	R472S	possibly damaging	Het
Tmprss11a	G	A	5: 86,576,494 (GRCm39)	T119M	probably benign	Het
Traip	C	T	9: 107,838,240 (GRCm39)	R142*	probably null	Het
Utrn	T	C	10: 12,626,214 (GRCm39)	N100D	probably damaging	Het
Vmn1r74	T	C	7: 11,581,575 (GRCm39)	S292P	probably benign	Het
Vmn2r71	A	T	7: 85,273,108 (GRCm39)	I641F	probably damaging	Het
Vmn2r9	T	A	5: 108,996,912 (GRCm39)	Y119F	possibly damaging	Het
Vmn2r98	A	G	17: 19,285,510 (GRCm39)	N110S	probably damaging	Het
Vwce	A	G	19: 10,642,057 (GRCm39)	T928A	probably benign	Het
Zfp608	T	A	18: 55,121,337 (GRCm39)	K83N	probably damaging	Het
Zfp808	A	G	13: 62,320,982 (GRCm39)	H737R	probably benign	Het
Zswim4	T	C	8: 84,940,714 (GRCm39)	N795S	probably benign	Het

Other mutations in Kcnma1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01318:Kcnma1	APN	14	23,364,390 (GRCm39)	splice site	probably benign
IGL01520:Kcnma1	APN	14	23,551,211 (GRCm39)	missense	possibly damaging	0.94
IGL01977:Kcnma1	APN	14	23,580,367 (GRCm39)	splice site	probably benign
IGL02140:Kcnma1	APN	14	23,359,113 (GRCm39)	missense	probably damaging	1.00
IGL02165:Kcnma1	APN	14	23,387,035 (GRCm39)	missense	possibly damaging	0.93
IGL02186:Kcnma1	APN	14	23,576,881 (GRCm39)	missense	probably benign	0.28
IGL02268:Kcnma1	APN	14	23,593,144 (GRCm39)	missense	probably damaging	1.00
IGL02353:Kcnma1	APN	14	23,641,681 (GRCm39)	missense	probably damaging	1.00
IGL02360:Kcnma1	APN	14	23,641,681 (GRCm39)	missense	probably damaging	1.00
IGL02491:Kcnma1	APN	14	23,361,757 (GRCm39)	missense	probably damaging	1.00
IGL02552:Kcnma1	APN	14	23,436,327 (GRCm39)	critical splice donor site	probably null
IGL02625:Kcnma1	APN	14	23,413,900 (GRCm39)	missense	probably damaging	1.00
IGL02677:Kcnma1	APN	14	23,513,224 (GRCm39)	missense	probably damaging	1.00
IGL02706:Kcnma1	APN	14	23,359,222 (GRCm39)	missense	probably damaging	1.00
G1citation:Kcnma1	UTSW	14	24,053,812 (GRCm39)	splice site	probably null
PIT4495001:Kcnma1	UTSW	14	23,475,665 (GRCm39)	missense	probably benign	0.00
PIT4514001:Kcnma1	UTSW	14	23,359,103 (GRCm39)	splice site	probably null
PIT4576001:Kcnma1	UTSW	14	23,359,103 (GRCm39)	splice site	probably null
R0071:Kcnma1	UTSW	14	23,576,835 (GRCm39)	missense	probably damaging	1.00
R0071:Kcnma1	UTSW	14	23,576,835 (GRCm39)	missense	probably damaging	1.00
R0115:Kcnma1	UTSW	14	23,364,243 (GRCm39)	missense	probably damaging	1.00
R0172:Kcnma1	UTSW	14	23,853,234 (GRCm39)	missense	probably damaging	1.00
R0178:Kcnma1	UTSW	14	23,576,835 (GRCm39)	missense	probably damaging	1.00
R0183:Kcnma1	UTSW	14	23,558,120 (GRCm39)	missense	probably damaging	1.00
R0240:Kcnma1	UTSW	14	23,544,647 (GRCm39)	missense	probably damaging	1.00
R0240:Kcnma1	UTSW	14	23,544,647 (GRCm39)	missense	probably damaging	1.00
R0328:Kcnma1	UTSW	14	23,423,265 (GRCm39)	missense	probably damaging	1.00
R0501:Kcnma1	UTSW	14	23,361,784 (GRCm39)	missense	possibly damaging	0.80
R0631:Kcnma1	UTSW	14	23,559,852 (GRCm39)	splice site	probably benign
R0668:Kcnma1	UTSW	14	23,417,563 (GRCm39)	missense	probably damaging	1.00
R0811:Kcnma1	UTSW	14	23,350,086 (GRCm39)	missense	probably damaging	0.96
R0812:Kcnma1	UTSW	14	23,350,086 (GRCm39)	missense	probably damaging	0.96
R1080:Kcnma1	UTSW	14	23,544,675 (GRCm39)	missense	probably damaging	1.00
R1419:Kcnma1	UTSW	14	23,417,710 (GRCm39)	missense	probably damaging	0.99
R1446:Kcnma1	UTSW	14	23,361,792 (GRCm39)	missense	probably damaging	1.00
R1454:Kcnma1	UTSW	14	23,513,268 (GRCm39)	missense	probably damaging	1.00
R1651:Kcnma1	UTSW	14	23,364,262 (GRCm39)	missense	probably damaging	1.00
R1826:Kcnma1	UTSW	14	23,380,997 (GRCm39)	missense	probably damaging	1.00
R1827:Kcnma1	UTSW	14	23,380,997 (GRCm39)	missense	probably damaging	1.00
R1828:Kcnma1	UTSW	14	23,380,997 (GRCm39)	missense	probably damaging	1.00
R1864:Kcnma1	UTSW	14	23,853,230 (GRCm39)	missense	probably damaging	1.00
R2002:Kcnma1	UTSW	14	23,387,097 (GRCm39)	missense	probably damaging	0.99
R2140:Kcnma1	UTSW	14	23,364,288 (GRCm39)	missense	probably damaging	1.00
R2278:Kcnma1	UTSW	14	23,593,151 (GRCm39)	nonsense	probably null
R2866:Kcnma1	UTSW	14	23,423,275 (GRCm39)	missense	probably benign	0.16
R2867:Kcnma1	UTSW	14	23,423,275 (GRCm39)	missense	probably benign	0.16
R2867:Kcnma1	UTSW	14	23,423,275 (GRCm39)	missense	probably benign	0.16
R2900:Kcnma1	UTSW	14	23,853,228 (GRCm39)	missense	probably damaging	1.00
R3820:Kcnma1	UTSW	14	23,350,006 (GRCm39)	missense	possibly damaging	0.66
R3821:Kcnma1	UTSW	14	23,417,679 (GRCm39)	missense	probably damaging	1.00
R3901:Kcnma1	UTSW	14	23,555,323 (GRCm39)	missense	probably damaging	0.98
R3975:Kcnma1	UTSW	14	24,053,815 (GRCm39)	critical splice donor site	probably null
R3976:Kcnma1	UTSW	14	24,053,815 (GRCm39)	critical splice donor site	probably null
R4352:Kcnma1	UTSW	14	23,361,720 (GRCm39)	missense	probably damaging	1.00
R4517:Kcnma1	UTSW	14	23,387,097 (GRCm39)	missense	probably damaging	1.00
R4598:Kcnma1	UTSW	14	23,853,228 (GRCm39)	missense	probably damaging	1.00
R4604:Kcnma1	UTSW	14	23,359,106 (GRCm39)	critical splice donor site	probably null
R4743:Kcnma1	UTSW	14	23,853,270 (GRCm39)	missense	probably damaging	1.00
R4754:Kcnma1	UTSW	14	23,413,904 (GRCm39)	missense	probably damaging	0.96
R4908:Kcnma1	UTSW	14	23,359,220 (GRCm39)	missense	probably damaging	0.99
R4960:Kcnma1	UTSW	14	24,054,186 (GRCm39)	intron	probably benign
R5175:Kcnma1	UTSW	14	23,386,106 (GRCm39)	critical splice donor site	probably null
R5218:Kcnma1	UTSW	14	23,513,253 (GRCm39)	missense	probably damaging	0.96
R5435:Kcnma1	UTSW	14	23,578,472 (GRCm39)	nonsense	probably null
R5705:Kcnma1	UTSW	14	24,053,839 (GRCm39)	missense	possibly damaging	0.73
R5746:Kcnma1	UTSW	14	23,544,635 (GRCm39)	missense	probably damaging	1.00
R5780:Kcnma1	UTSW	14	23,436,419 (GRCm39)	nonsense	probably null
R5793:Kcnma1	UTSW	14	23,359,103 (GRCm39)	splice site	probably null
R6039:Kcnma1	UTSW	14	23,359,105 (GRCm39)	missense	probably benign	0.42
R6039:Kcnma1	UTSW	14	23,359,105 (GRCm39)	missense	probably benign	0.42
R6133:Kcnma1	UTSW	14	24,053,936 (GRCm39)	missense	probably damaging	0.98
R6271:Kcnma1	UTSW	14	23,559,957 (GRCm39)	missense	probably damaging	1.00
R6490:Kcnma1	UTSW	14	23,386,165 (GRCm39)	missense	possibly damaging	0.46
R6704:Kcnma1	UTSW	14	24,052,882 (GRCm39)	nonsense	probably null
R6822:Kcnma1	UTSW	14	24,053,812 (GRCm39)	splice site	probably null
R6855:Kcnma1	UTSW	14	23,417,679 (GRCm39)	missense	probably damaging	1.00
R7017:Kcnma1	UTSW	14	23,544,711 (GRCm39)	missense	possibly damaging	0.79
R7081:Kcnma1	UTSW	14	23,350,086 (GRCm39)	missense	probably damaging	0.96
R7113:Kcnma1	UTSW	14	23,513,224 (GRCm39)	missense	probably damaging	1.00
R7131:Kcnma1	UTSW	14	23,417,562 (GRCm39)	missense	probably damaging	1.00
R7172:Kcnma1	UTSW	14	23,576,691 (GRCm39)	missense	probably damaging	1.00
R7207:Kcnma1	UTSW	14	23,359,083 (GRCm39)	makesense	probably null
R7308:Kcnma1	UTSW	14	23,381,003 (GRCm39)	missense	probably damaging	0.99
R7371:Kcnma1	UTSW	14	23,544,638 (GRCm39)	missense	possibly damaging	0.94
R7404:Kcnma1	UTSW	14	24,052,902 (GRCm39)	missense	unknown
R7560:Kcnma1	UTSW	14	23,580,310 (GRCm39)	missense	probably benign	0.15
R7693:Kcnma1	UTSW	14	23,417,680 (GRCm39)	missense	probably damaging	1.00
R7763:Kcnma1	UTSW	14	23,350,074 (GRCm39)	missense	possibly damaging	0.66
R7809:Kcnma1	UTSW	14	23,423,324 (GRCm39)	missense	probably benign	0.16
R7832:Kcnma1	UTSW	14	23,440,991 (GRCm39)	missense	probably benign
R7884:Kcnma1	UTSW	14	23,387,057 (GRCm39)	missense	probably benign	0.01
R8013:Kcnma1	UTSW	14	23,423,211 (GRCm39)	missense	probably benign	0.31
R8014:Kcnma1	UTSW	14	23,423,211 (GRCm39)	missense	probably benign	0.31
R8066:Kcnma1	UTSW	14	23,361,744 (GRCm39)	missense	probably benign	0.00
R8097:Kcnma1	UTSW	14	23,381,032 (GRCm39)	missense	probably damaging	1.00
R8154:Kcnma1	UTSW	14	23,361,822 (GRCm39)	missense	possibly damaging	0.62
R8507:Kcnma1	UTSW	14	23,641,706 (GRCm39)	missense	probably benign	0.00
R8672:Kcnma1	UTSW	14	23,551,230 (GRCm39)	missense	probably damaging	1.00
R8677:Kcnma1	UTSW	14	23,436,418 (GRCm39)	missense	probably benign	0.36
R8725:Kcnma1	UTSW	14	23,436,332 (GRCm39)	missense	probably benign	0.00
R8727:Kcnma1	UTSW	14	23,436,332 (GRCm39)	missense	probably benign	0.00
R8827:Kcnma1	UTSW	14	23,417,548 (GRCm39)	missense	probably damaging	1.00
R8880:Kcnma1	UTSW	14	23,417,718 (GRCm39)	missense	probably damaging	1.00
R8997:Kcnma1	UTSW	14	23,513,037 (GRCm39)	intron	probably benign
R9056:Kcnma1	UTSW	14	23,700,214 (GRCm39)	missense	possibly damaging	0.80
R9346:Kcnma1	UTSW	14	23,700,233 (GRCm39)	missense	possibly damaging	0.94
R9403:Kcnma1	UTSW	14	23,593,145 (GRCm39)	missense	probably benign	0.05
R9438:Kcnma1	UTSW	14	23,417,653 (GRCm39)	missense	probably benign	0.00
R9482:Kcnma1	UTSW	14	23,441,033 (GRCm39)	missense	probably benign
R9511:Kcnma1	UTSW	14	23,361,793 (GRCm39)	missense	possibly damaging	0.90
R9649:Kcnma1	UTSW	14	23,501,666 (GRCm39)	critical splice donor site	probably null
R9663:Kcnma1	UTSW	14	24,053,897 (GRCm39)	missense	probably benign	0.15
R9673:Kcnma1	UTSW	14	23,558,123 (GRCm39)	missense	probably benign	0.01
RF001:Kcnma1	UTSW	14	23,361,765 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGACACTGTAAATGGTCACAAGAC -3'
(R):5'- TATAGCGCGGTTAGTGGAAG -3'

Sequencing Primer
(F):5'- GTCACAAGACCTCAAGATGGTC -3'
(R):5'- CCAAGAGGTTTTTGTGGCCTC -3'

Posted On

2018-11-06