Mutagenetix > Incidental Mutation

Incidental Mutation 'R6580:Vmn2r84'

524096

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r84

Ensembl Gene

ENSMUSG00000070601

Gene Name

vomeronasal 2, receptor 84

Synonyms

EG625068

MMRRC Submission

044704-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R6580 (G1)

Quality Score

225.009

Status

Not validated

Chromosome

Chromosomal Location

130221669-130230110 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 130225110 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Tryptophan to Arginine at position 467 (W467R)

Ref Sequence

ENSEMBL: ENSMUSP00000092079 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094502]

AlphaFold

D3YWE3

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094502
AA Change: W467R

PolyPhen 2 Score 0.933 (Sensitivity: 0.80; Specificity: 0.94)

SMART Domains

Protein: ENSMUSP00000092079
Gene: ENSMUSG00000070601
AA Change: W467R

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
Pfam:ANF_receptor	77	448	1.3e-27	PFAM
Pfam:NCD3G	508	561	6.9e-21	PFAM
Pfam:7tm_3	594	830	4.6e-55	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 93.2%

Validation Efficiency

97% (32/33)

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	G	A	3: 137,772,386 (GRCm39)	R525H	probably benign	Het
Acox3	T	A	5: 35,765,747 (GRCm39)	L600Q	probably damaging	Het
Ankrd49	A	G	9: 14,692,694 (GRCm39)	S157P	probably damaging	Het
Ccdc141	A	G	2: 76,842,099 (GRCm39)	F1444S	possibly damaging	Het
Cimap2	G	T	4: 106,468,711 (GRCm39)	H271N	possibly damaging	Het
Defb28	C	T	2: 152,360,215 (GRCm39)	S10L	possibly damaging	Het
Epha6	T	G	16: 59,502,979 (GRCm39)	N976T	probably damaging	Het
Gm45861	A	G	8: 28,034,979 (GRCm39)	K976E	unknown	Het
Gtf2ird1	T	G	5: 134,389,893 (GRCm39)	N920H	probably damaging	Het
Gtf3c1	A	T	7: 125,243,519 (GRCm39)	M1695K	probably benign	Het
Hfm1	C	T	5: 106,995,575 (GRCm39)	E1279K	probably benign	Het
Il31ra	C	T	13: 112,688,476 (GRCm39)	D34N	possibly damaging	Het
Klhl3	T	A	13: 58,166,701 (GRCm39)	I430F	possibly damaging	Het
Mfhas1	A	G	8: 36,056,419 (GRCm39)	Y298C	probably damaging	Het
Muc20	A	T	16: 32,613,859 (GRCm39)	M506K	possibly damaging	Het
Myo1c	C	T	11: 75,562,461 (GRCm39)	P918S	probably benign	Het
Naip1	T	A	13: 100,581,157 (GRCm39)	D30V	probably damaging	Het
Nol9	A	G	4: 152,136,218 (GRCm39)	N430S	probably benign	Het
Or8b44	T	C	9: 38,410,319 (GRCm39)	M118T	probably damaging	Het
Palm3	A	G	8: 84,756,177 (GRCm39)	E563G	probably damaging	Het
Pcdhga4	A	G	18: 37,820,370 (GRCm39)	S640G	possibly damaging	Het
Pi4ka	A	G	16: 17,168,694 (GRCm39)	F679L	probably damaging	Het
Pierce1	A	G	2: 28,356,062 (GRCm39)	W74R	probably damaging	Het
Polr3c	A	G	3: 96,634,659 (GRCm39)		probably null	Het
Ptdss2	T	A	7: 140,732,925 (GRCm39)	I236N	probably damaging	Het
Rapgef1	A	G	2: 29,620,621 (GRCm39)	Y879C	possibly damaging	Het
Shc3	T	C	13: 51,596,809 (GRCm39)	T405A	probably benign	Het
Smtnl2	G	T	11: 72,293,859 (GRCm39)	S232R	probably benign	Het
Taar8a	G	A	10: 23,952,791 (GRCm39)	A132T	probably damaging	Het
Tex47	T	C	5: 7,355,212 (GRCm39)	I131T	probably damaging	Het
Tiam2	CGGG	CGGGG	17: 3,464,897 (GRCm39)		probably null	Het
Vmn1r63	A	G	7: 5,805,913 (GRCm39)	S240P	probably benign	Het
Vmn2r130	T	C	17: 23,282,740 (GRCm39)	V140A	probably benign	Het
Zscan12	T	C	13: 21,553,328 (GRCm39)	L384P	probably damaging	Het

Other mutations in Vmn2r84

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01461:Vmn2r84	APN	10	130,227,094 (GRCm39)	missense	possibly damaging	0.65
IGL01590:Vmn2r84	APN	10	130,221,964 (GRCm39)	missense	probably damaging	1.00
IGL01639:Vmn2r84	APN	10	130,225,141 (GRCm39)	nonsense	probably null
IGL01843:Vmn2r84	APN	10	130,222,148 (GRCm39)	missense	probably benign
IGL01911:Vmn2r84	APN	10	130,222,277 (GRCm39)	missense	probably damaging	0.99
IGL01937:Vmn2r84	APN	10	130,221,755 (GRCm39)	missense	probably damaging	1.00
IGL01977:Vmn2r84	APN	10	130,229,935 (GRCm39)	missense	probably benign	0.11
IGL02177:Vmn2r84	APN	10	130,227,881 (GRCm39)	missense	probably benign	0.00
IGL02291:Vmn2r84	APN	10	130,226,617 (GRCm39)	missense	probably damaging	1.00
IGL02590:Vmn2r84	APN	10	130,227,356 (GRCm39)	splice site	probably benign
IGL02727:Vmn2r84	APN	10	130,229,995 (GRCm39)	missense	possibly damaging	0.95
IGL02900:Vmn2r84	APN	10	130,223,861 (GRCm39)	splice site	probably benign
IGL03383:Vmn2r84	APN	10	130,222,556 (GRCm39)	missense	probably damaging	1.00
PIT4378001:Vmn2r84	UTSW	10	130,221,784 (GRCm39)	missense	probably damaging	1.00
R0076:Vmn2r84	UTSW	10	130,230,062 (GRCm39)	missense	probably damaging	1.00
R0089:Vmn2r84	UTSW	10	130,222,588 (GRCm39)	splice site	probably benign
R0153:Vmn2r84	UTSW	10	130,227,877 (GRCm39)	missense	probably benign	0.06
R0611:Vmn2r84	UTSW	10	130,221,991 (GRCm39)	missense	probably damaging	1.00
R0883:Vmn2r84	UTSW	10	130,226,984 (GRCm39)	missense	probably damaging	0.99
R1237:Vmn2r84	UTSW	10	130,223,725 (GRCm39)	splice site	probably null
R1295:Vmn2r84	UTSW	10	130,225,008 (GRCm39)	missense	probably benign	0.12
R1401:Vmn2r84	UTSW	10	130,227,859 (GRCm39)	missense	possibly damaging	0.89
R1521:Vmn2r84	UTSW	10	130,225,137 (GRCm39)	missense	probably benign	0.10
R1590:Vmn2r84	UTSW	10	130,227,349 (GRCm39)	critical splice acceptor site	probably null
R1710:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R1891:Vmn2r84	UTSW	10	130,221,938 (GRCm39)	missense	possibly damaging	0.78
R1956:Vmn2r84	UTSW	10	130,226,677 (GRCm39)	missense	probably benign	0.01
R1957:Vmn2r84	UTSW	10	130,226,677 (GRCm39)	missense	probably benign	0.01
R1962:Vmn2r84	UTSW	10	130,226,591 (GRCm39)	missense	probably damaging	0.99
R1994:Vmn2r84	UTSW	10	130,221,878 (GRCm39)	missense	probably damaging	1.00
R2124:Vmn2r84	UTSW	10	130,227,100 (GRCm39)	missense	probably damaging	0.99
R2409:Vmn2r84	UTSW	10	130,227,940 (GRCm39)	missense	probably damaging	0.99
R2474:Vmn2r84	UTSW	10	130,222,392 (GRCm39)	missense	possibly damaging	0.50
R2851:Vmn2r84	UTSW	10	130,230,036 (GRCm39)	missense	probably benign	0.05
R3508:Vmn2r84	UTSW	10	130,226,777 (GRCm39)	missense	probably damaging	1.00
R3792:Vmn2r84	UTSW	10	130,221,669 (GRCm39)	makesense	probably null
R4051:Vmn2r84	UTSW	10	130,226,767 (GRCm39)	missense	probably damaging	1.00
R4061:Vmn2r84	UTSW	10	130,221,898 (GRCm39)	missense	probably damaging	1.00
R4091:Vmn2r84	UTSW	10	130,227,238 (GRCm39)	missense	probably damaging	1.00
R4190:Vmn2r84	UTSW	10	130,227,163 (GRCm39)	nonsense	probably null
R4520:Vmn2r84	UTSW	10	130,222,391 (GRCm39)	missense	probably damaging	1.00
R4584:Vmn2r84	UTSW	10	130,226,582 (GRCm39)	missense	probably benign	0.00
R4588:Vmn2r84	UTSW	10	130,221,809 (GRCm39)	missense	probably damaging	0.98
R4655:Vmn2r84	UTSW	10	130,229,973 (GRCm39)	nonsense	probably null
R4860:Vmn2r84	UTSW	10	130,221,712 (GRCm39)	missense	probably damaging	0.99
R4860:Vmn2r84	UTSW	10	130,221,712 (GRCm39)	missense	probably damaging	0.99
R5022:Vmn2r84	UTSW	10	130,222,417 (GRCm39)	missense	probably damaging	1.00
R5146:Vmn2r84	UTSW	10	130,221,971 (GRCm39)	missense	probably damaging	1.00
R5237:Vmn2r84	UTSW	10	130,221,863 (GRCm39)	missense	probably damaging	0.99
R5695:Vmn2r84	UTSW	10	130,225,064 (GRCm39)	missense	probably benign	0.12
R5793:Vmn2r84	UTSW	10	130,221,754 (GRCm39)	missense	probably damaging	0.99
R6210:Vmn2r84	UTSW	10	130,222,114 (GRCm39)	missense	probably damaging	1.00
R6286:Vmn2r84	UTSW	10	130,226,737 (GRCm39)	missense	possibly damaging	0.65
R6607:Vmn2r84	UTSW	10	130,226,731 (GRCm39)	missense	possibly damaging	0.87
R6818:Vmn2r84	UTSW	10	130,222,147 (GRCm39)	missense	probably benign	0.09
R6956:Vmn2r84	UTSW	10	130,225,136 (GRCm39)	missense	probably damaging	0.98
R6994:Vmn2r84	UTSW	10	130,226,876 (GRCm39)	missense	possibly damaging	0.90
R7075:Vmn2r84	UTSW	10	130,226,941 (GRCm39)	missense	probably damaging	0.99
R7225:Vmn2r84	UTSW	10	130,222,552 (GRCm39)	missense	probably damaging	0.99
R7252:Vmn2r84	UTSW	10	130,222,279 (GRCm39)	missense	probably damaging	1.00
R7263:Vmn2r84	UTSW	10	130,225,077 (GRCm39)	missense	probably damaging	1.00
R7297:Vmn2r84	UTSW	10	130,227,119 (GRCm39)	missense	probably benign	0.19
R7439:Vmn2r84	UTSW	10	130,227,982 (GRCm39)	missense	possibly damaging	0.90
R7441:Vmn2r84	UTSW	10	130,227,982 (GRCm39)	missense	possibly damaging	0.90
R7857:Vmn2r84	UTSW	10	130,226,738 (GRCm39)	missense	probably benign	0.00
R8263:Vmn2r84	UTSW	10	130,227,037 (GRCm39)	missense	probably damaging	1.00
R8555:Vmn2r84	UTSW	10	130,230,100 (GRCm39)	missense	probably benign	0.28
R8766:Vmn2r84	UTSW	10	130,222,241 (GRCm39)	missense	probably damaging	0.98
R8821:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R8831:Vmn2r84	UTSW	10	130,226,968 (GRCm39)	missense	probably benign	0.02
R8970:Vmn2r84	UTSW	10	130,222,244 (GRCm39)	missense	probably damaging	0.98
R9164:Vmn2r84	UTSW	10	130,221,669 (GRCm39)	makesense	probably null
R9190:Vmn2r84	UTSW	10	130,226,843 (GRCm39)	missense	probably benign	0.03
R9261:Vmn2r84	UTSW	10	130,229,976 (GRCm39)	missense	probably benign	0.00
R9310:Vmn2r84	UTSW	10	130,227,993 (GRCm39)	missense	possibly damaging	0.81
R9434:Vmn2r84	UTSW	10	130,221,745 (GRCm39)	missense	possibly damaging	0.65
R9613:Vmn2r84	UTSW	10	130,226,591 (GRCm39)	missense	probably damaging	0.99
Z1177:Vmn2r84	UTSW	10	130,227,771 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ACAAGTGAAAACCTTGAATCTGGAG -3'
(R):5'- GGGGAAATTCTCAACTTTGCAC -3'

Sequencing Primer
(F):5'- AATCCAGAGGCCTTTGATGC -3'
(R):5'- ACCCTGTTGGAGAAATGG -3'

Posted On

2018-06-22