Mutagenetix > Incidental Mutation

Incidental Mutation 'R6580:Taar8a'

524094

Institutional Source

Beutler Lab

Gene Symbol

Taar8a

Ensembl Gene

ENSMUSG00000096442

Gene Name

trace amine-associated receptor 8A

Synonyms

LOC215859

MMRRC Submission

044704-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R6580 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

23952398-23953432 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 23952791 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Threonine at position 132 (A132T)

Ref Sequence

ENSEMBL: ENSMUSP00000062719 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000051133]

AlphaFold

Q5QD07

Predicted Effect

probably damaging
Transcript: ENSMUST00000051133
AA Change: A132T

PolyPhen 2 Score 0.987 (Sensitivity: 0.73; Specificity: 0.96)

SMART Domains

Protein: ENSMUSP00000062719
Gene: ENSMUSG00000096442
AA Change: A132T

Domain	Start	End	E-Value	Type
Pfam:7TM_GPCR_Srx	40	244	3.9e-8	PFAM
Pfam:7TM_GPCR_Srsx	42	327	7.1e-15	PFAM
Pfam:7tm_1	48	312	9.1e-58	PFAM

Meta Mutation Damage Score

0.6467

Coding Region Coverage

1x: 99.9%
3x: 99.5%
10x: 97.7%
20x: 93.2%

Validation Efficiency

97% (32/33)

Allele List at MGI

Other mutations in this stock

Total: 34 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	G	A	3: 137,772,386 (GRCm39)	R525H	probably benign	Het
Acox3	T	A	5: 35,765,747 (GRCm39)	L600Q	probably damaging	Het
Ankrd49	A	G	9: 14,692,694 (GRCm39)	S157P	probably damaging	Het
Ccdc141	A	G	2: 76,842,099 (GRCm39)	F1444S	possibly damaging	Het
Cimap2	G	T	4: 106,468,711 (GRCm39)	H271N	possibly damaging	Het
Defb28	C	T	2: 152,360,215 (GRCm39)	S10L	possibly damaging	Het
Epha6	T	G	16: 59,502,979 (GRCm39)	N976T	probably damaging	Het
Gm45861	A	G	8: 28,034,979 (GRCm39)	K976E	unknown	Het
Gtf2ird1	T	G	5: 134,389,893 (GRCm39)	N920H	probably damaging	Het
Gtf3c1	A	T	7: 125,243,519 (GRCm39)	M1695K	probably benign	Het
Hfm1	C	T	5: 106,995,575 (GRCm39)	E1279K	probably benign	Het
Il31ra	C	T	13: 112,688,476 (GRCm39)	D34N	possibly damaging	Het
Klhl3	T	A	13: 58,166,701 (GRCm39)	I430F	possibly damaging	Het
Mfhas1	A	G	8: 36,056,419 (GRCm39)	Y298C	probably damaging	Het
Muc20	A	T	16: 32,613,859 (GRCm39)	M506K	possibly damaging	Het
Myo1c	C	T	11: 75,562,461 (GRCm39)	P918S	probably benign	Het
Naip1	T	A	13: 100,581,157 (GRCm39)	D30V	probably damaging	Het
Nol9	A	G	4: 152,136,218 (GRCm39)	N430S	probably benign	Het
Or8b44	T	C	9: 38,410,319 (GRCm39)	M118T	probably damaging	Het
Palm3	A	G	8: 84,756,177 (GRCm39)	E563G	probably damaging	Het
Pcdhga4	A	G	18: 37,820,370 (GRCm39)	S640G	possibly damaging	Het
Pi4ka	A	G	16: 17,168,694 (GRCm39)	F679L	probably damaging	Het
Pierce1	A	G	2: 28,356,062 (GRCm39)	W74R	probably damaging	Het
Polr3c	A	G	3: 96,634,659 (GRCm39)		probably null	Het
Ptdss2	T	A	7: 140,732,925 (GRCm39)	I236N	probably damaging	Het
Rapgef1	A	G	2: 29,620,621 (GRCm39)	Y879C	possibly damaging	Het
Shc3	T	C	13: 51,596,809 (GRCm39)	T405A	probably benign	Het
Smtnl2	G	T	11: 72,293,859 (GRCm39)	S232R	probably benign	Het
Tex47	T	C	5: 7,355,212 (GRCm39)	I131T	probably damaging	Het
Tiam2	CGGG	CGGGG	17: 3,464,897 (GRCm39)		probably null	Het
Vmn1r63	A	G	7: 5,805,913 (GRCm39)	S240P	probably benign	Het
Vmn2r130	T	C	17: 23,282,740 (GRCm39)	V140A	probably benign	Het
Vmn2r84	A	G	10: 130,225,110 (GRCm39)	W467R	possibly damaging	Het
Zscan12	T	C	13: 21,553,328 (GRCm39)	L384P	probably damaging	Het

Other mutations in Taar8a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01118:Taar8a	APN	10	23,952,759 (GRCm39)	missense	probably damaging	1.00
IGL01546:Taar8a	APN	10	23,952,813 (GRCm39)	missense	possibly damaging	0.95
IGL01597:Taar8a	APN	10	23,952,756 (GRCm39)	missense	possibly damaging	0.96
IGL02387:Taar8a	APN	10	23,953,260 (GRCm39)	missense	possibly damaging	0.93
IGL02815:Taar8a	APN	10	23,953,278 (GRCm39)	missense	probably benign	0.02
R0762:Taar8a	UTSW	10	23,952,975 (GRCm39)	missense	probably benign	0.34
R1690:Taar8a	UTSW	10	23,952,813 (GRCm39)	missense	probably damaging	1.00
R2891:Taar8a	UTSW	10	23,953,028 (GRCm39)	missense	probably benign	0.02
R3889:Taar8a	UTSW	10	23,952,923 (GRCm39)	missense	probably benign	0.00
R3962:Taar8a	UTSW	10	23,953,054 (GRCm39)	missense	probably damaging	0.99
R4090:Taar8a	UTSW	10	23,953,062 (GRCm39)	missense	probably damaging	0.96
R6605:Taar8a	UTSW	10	23,952,674 (GRCm39)	missense	possibly damaging	0.88
R7422:Taar8a	UTSW	10	23,952,762 (GRCm39)	missense	probably damaging	1.00
R7571:Taar8a	UTSW	10	23,953,306 (GRCm39)	nonsense	probably null
R8265:Taar8a	UTSW	10	23,952,839 (GRCm39)	missense	probably damaging	1.00
R9317:Taar8a	UTSW	10	23,952,753 (GRCm39)	missense	probably benign	0.00
R9371:Taar8a	UTSW	10	23,952,753 (GRCm39)	missense	probably benign	0.00
R9710:Taar8a	UTSW	10	23,952,714 (GRCm39)	missense	probably damaging	0.97
R9755:Taar8a	UTSW	10	23,952,995 (GRCm39)	missense	probably benign	0.01
X0022:Taar8a	UTSW	10	23,953,406 (GRCm39)	missense	possibly damaging	0.93
X0062:Taar8a	UTSW	10	23,953,279 (GRCm39)	nonsense	probably null

Predicted Primers

PCR Primer
(F):5'- TTTCAAGCAGCTGCACTCTC -3'
(R):5'- GATAACAATTTGGCAGCCCC -3'

Sequencing Primer
(F):5'- CTCTCTGGCCAGTGCTGAC -3'
(R):5'- TGGCAGCCCCCTACACAATTTAG -3'

Posted On

2018-06-22