Mutagenetix > Incidental Mutation

Incidental Mutation 'R6170:Tmem210'

490356

Institutional Source

Beutler Lab

Gene Symbol

Tmem210

Ensembl Gene

ENSMUSG00000026963

Gene Name

transmembrane protein 210

Synonyms

4930571C24Rik

MMRRC Submission

044431-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.102) question?

Stock #

R6170 (G1)

Quality Score

225.009

Status

Validated

Chromosome

Chromosomal Location

25178157-25179201 bp(+) (GRCm39)

Type of Mutation

critical splice acceptor site

DNA Base Change (assembly)

G to A at 25178776 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000028340 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000028335] [ENSMUST00000028337] [ENSMUST00000028340] [ENSMUST00000028341] [ENSMUST00000114307] [ENSMUST00000114308] [ENSMUST00000114310] [ENSMUST00000114318] [ENSMUST00000114312] [ENSMUST00000114314] [ENSMUST00000114317]

AlphaFold

Q9D2F0

Predicted Effect

probably benign
Transcript: ENSMUST00000028335

SMART Domains

Protein: ENSMUSP00000028335
Gene: ENSMUSG00000026959

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	38	357	6.6e-35	PFAM
PBPe	433	795	2.71e-97	SMART
Lig_chan-Glu_bd	439	507	2.99e-18	SMART
Pfam:CaM_bdg_C0	835	863	1.5e-18	PFAM
PDB:3BYA\|B	875	898	4e-6	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000028337

SMART Domains

Protein: ENSMUSP00000028337
Gene: ENSMUSG00000026961

Domain	Start	End	E-Value	Type
signal peptide	1	26	N/A	INTRINSIC
LRRNT	42	75	1.86e0	SMART
LRR	72	93	7.57e0	SMART
LRR	95	117	6.96e0	SMART
LRR_TYP	118	141	1e-5	SMART
LRR	142	165	6.22e0	SMART
LRR	166	189	2.86e-1	SMART
LRRCT	201	254	3.01e-5	SMART
transmembrane domain	267	289	N/A	INTRINSIC
low complexity region	293	308	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000028340

SMART Domains

Protein: ENSMUSP00000028340
Gene: ENSMUSG00000026963

Domain	Start	End	E-Value	Type
signal peptide	1	30	N/A	INTRINSIC
Pfam:TMEM210	32	144	1.1e-65	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000028341

SMART Domains

Protein: ENSMUSP00000028341
Gene: ENSMUSG00000026965

Domain	Start	End	E-Value	Type
low complexity region	6	19	N/A	INTRINSIC
low complexity region	123	133	N/A	INTRINSIC
low complexity region	153	164	N/A	INTRINSIC
low complexity region	221	229	N/A	INTRINSIC
low complexity region	456	467	N/A	INTRINSIC
CULLIN	515	663	6.72e-9	SMART
APC2	772	832	3.67e-27	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000114307

SMART Domains

Protein: ENSMUSP00000109946
Gene: ENSMUSG00000026959

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	38	357	1e-34	PFAM
PBPe	433	795	2.71e-97	SMART
Lig_chan-Glu_bd	439	507	2.99e-18	SMART
Pfam:CaM_bdg_C0	835	863	3.2e-19	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000114308

SMART Domains

Protein: ENSMUSP00000109947
Gene: ENSMUSG00000026959

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	38	378	8e-31	PFAM
PBPe	454	816	2.71e-97	SMART
Lig_chan-Glu_bd	460	528	2.99e-18	SMART
Pfam:CaM_bdg_C0	856	884	3.3e-19	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000114310

SMART Domains

Protein: ENSMUSP00000109949
Gene: ENSMUSG00000026959

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	39	299	3.6e-24	PFAM
Blast:PBPe	352	420	9e-37	BLAST
PBPe	454	816	2.71e-97	SMART
Lig_chan-Glu_bd	460	528	2.99e-18	SMART
Pfam:CaM_bdg_C0	856	884	8.4e-17	PFAM
PDB:3BYA\|B	896	919	4e-6	PDB

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000144102

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000127171

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000129723

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000135426

Predicted Effect

probably benign
Transcript: ENSMUST00000114318

SMART Domains

Protein: ENSMUSP00000109957
Gene: ENSMUSG00000026959

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	38	378	8.4e-31	PFAM
PBPe	454	816	2.71e-97	SMART
Lig_chan-Glu_bd	460	528	2.99e-18	SMART
Pfam:CaM_bdg_C0	856	884	3.4e-19	PFAM
PDB:3BYA\|B	896	919	4e-6	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000114312

SMART Domains

Protein: ENSMUSP00000109951
Gene: ENSMUSG00000026959

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	38	357	5.9e-35	PFAM
PBPe	433	795	2.71e-97	SMART
Lig_chan-Glu_bd	439	507	2.99e-18	SMART
Pfam:CaM_bdg_C0	835	863	1.4e-18	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000114314

SMART Domains

Protein: ENSMUSP00000109953
Gene: ENSMUSG00000026959

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	38	357	1.1e-34	PFAM
PBPe	433	795	2.71e-97	SMART
Lig_chan-Glu_bd	439	507	2.99e-18	SMART
Pfam:CaM_bdg_C0	835	863	3.3e-19	PFAM
PDB:3BYA\|B	875	898	4e-6	PDB

Predicted Effect

probably benign
Transcript: ENSMUST00000114317

SMART Domains

Protein: ENSMUSP00000109956
Gene: ENSMUSG00000026959

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	38	378	7.7e-31	PFAM
PBPe	454	816	2.71e-97	SMART
Lig_chan-Glu_bd	460	528	2.99e-18	SMART
Pfam:CaM_bdg_C0	856	884	3.3e-19	PFAM

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 98.2%
20x: 94.8%

Validation Efficiency

96% (68/71)

Allele List at MGI

Other mutations in this stock

Total: 69 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
0610030E20Rik	T	A	6: 72,325,555 (GRCm39)	S90R	probably benign	Het
4930523C07Rik	C	A	1: 159,902,743 (GRCm39)	N4K	possibly damaging	Het
Adgrl2	A	G	3: 148,528,645 (GRCm39)	S1167P	probably damaging	Het
Akr1e1	T	C	13: 4,652,723 (GRCm39)	D94G	possibly damaging	Het
Anln	T	C	9: 22,279,793 (GRCm39)	N466D	probably benign	Het
Atp9b	T	A	18: 80,920,562 (GRCm39)	I231L	probably benign	Het
Bpifb3	T	A	2: 153,761,557 (GRCm39)	M2K	unknown	Het
Btbd3	T	C	2: 138,120,862 (GRCm39)	L12P	probably damaging	Het
Btnl6	T	A	17: 34,734,480 (GRCm39)	Y94F	probably damaging	Het
Cab39	T	A	1: 85,746,176 (GRCm39)	L19*	probably null	Het
Cacna2d2	A	G	9: 107,404,533 (GRCm39)	D1114G	probably damaging	Het
Cdcp3	A	T	7: 130,776,216 (GRCm39)		probably null	Het
Cdh11	A	G	8: 103,361,442 (GRCm39)	V632A	probably benign	Het
Cemip	T	G	7: 83,596,438 (GRCm39)	T1109P	possibly damaging	Het
Col7a1	C	A	9: 108,795,511 (GRCm39)	P1522Q	unknown	Het
Colgalt1	C	T	8: 72,074,514 (GRCm39)	L409F	probably damaging	Het
Crtc2	A	G	3: 90,166,907 (GRCm39)	M125V	probably benign	Het
Cyp2b19	T	G	7: 26,458,519 (GRCm39)	M78R	possibly damaging	Het
Cyp4a12a	A	C	4: 115,184,643 (GRCm39)	D308A	possibly damaging	Het
D16Ertd472e	T	C	16: 78,342,155 (GRCm39)	T242A	probably benign	Het
Ddah1	A	G	3: 145,597,261 (GRCm39)	D166G	probably benign	Het
Dmtn	T	C	14: 70,854,795 (GRCm39)	D60G	probably damaging	Het
Dsg1a	A	T	18: 20,469,043 (GRCm39)	D607V	probably damaging	Het
Ebf1	T	A	11: 44,774,712 (GRCm39)	N236K	probably damaging	Het
Emc1	A	G	4: 139,093,689 (GRCm39)	T600A	probably benign	Het
Fbxo33	T	C	12: 59,251,435 (GRCm39)	N360S	probably benign	Het
Fbxw5	A	G	2: 25,393,615 (GRCm39)	D72G	possibly damaging	Het
Fhad1	T	A	4: 141,618,263 (GRCm39)	K1388*	probably null	Het
Fzd7	A	G	1: 59,523,004 (GRCm39)	M296V	probably benign	Het
Gdpd3	T	C	7: 126,370,336 (GRCm39)	I257T	probably benign	Het
Glt28d2	T	G	3: 85,779,248 (GRCm39)	D75A	possibly damaging	Het
Gm14295	G	A	2: 176,502,937 (GRCm39)		probably benign	Het
Gm28729	A	G	9: 96,401,494 (GRCm39)	I98T	probably damaging	Het
Gm4924	C	T	10: 82,213,065 (GRCm39)	Q288*	probably null	Het
Gpr150	T	C	13: 76,204,676 (GRCm39)	M90V	probably damaging	Het
Ireb2	G	A	9: 54,794,656 (GRCm39)	V331I	probably benign	Het
Lpcat2b	A	T	5: 107,581,760 (GRCm39)	Y363F	probably benign	Het
Me2	T	C	18: 73,918,852 (GRCm39)	I410V	probably benign	Het
Naxe	T	C	3: 87,965,537 (GRCm39)	E58G	probably damaging	Het
Nlrp10	T	A	7: 108,523,671 (GRCm39)	D603V	probably benign	Het
Nlrp1b	T	A	11: 71,046,905 (GRCm39)	Y1149F	probably damaging	Het
Nrg1	A	G	8: 32,308,508 (GRCm39)	Y503H	probably damaging	Het
Nxpe4	C	T	9: 48,304,104 (GRCm39)	P64S	probably benign	Het
Pkd1l3	A	T	8: 110,349,811 (GRCm39)	T219S	unknown	Het
Plagl1	T	C	10: 13,002,975 (GRCm39)	L81P	probably damaging	Het
Poglut3	T	C	9: 53,311,042 (GRCm39)	V481A	possibly damaging	Het
Polq	A	G	16: 36,866,174 (GRCm39)	Q457R	possibly damaging	Het
Potefam1	A	G	2: 111,058,293 (GRCm39)	Y167H	probably benign	Het
Ppargc1a	C	A	5: 51,631,253 (GRCm39)	A459S	probably damaging	Het
Ppp1r13l	A	G	7: 19,104,362 (GRCm39)	D253G	probably benign	Het
Prl2c2	T	A	13: 13,176,757 (GRCm39)	N55Y	probably damaging	Het
Prlr	T	C	15: 10,328,935 (GRCm39)	F470S	probably benign	Het
Serpina12	T	C	12: 104,004,500 (GRCm39)	D44G	probably benign	Het
Sfxn1	T	G	13: 54,260,526 (GRCm39)	S291R	probably benign	Het
Sipa1l1	A	G	12: 82,388,446 (GRCm39)	D224G	probably benign	Het
Slc30a7	A	G	3: 115,784,392 (GRCm39)	F123S	probably damaging	Het
Spata31f1a	A	G	4: 42,849,345 (GRCm39)	V937A	probably benign	Het
Stox2	T	A	8: 47,645,055 (GRCm39)	M802L	probably benign	Het
Tmem150a	G	A	6: 72,333,728 (GRCm39)	R30H	probably benign	Het
Tor3a	T	C	1: 156,484,143 (GRCm39)	N269S	possibly damaging	Het
Trp63	A	C	16: 25,703,603 (GRCm39)	N423T	probably benign	Het
Vmn2r60	A	G	7: 41,785,045 (GRCm39)	I86V	possibly damaging	Het
Vmn2r74	T	C	7: 85,606,348 (GRCm39)	I333V	probably benign	Het
Vwa7	C	A	17: 35,240,186 (GRCm39)	H385N	possibly damaging	Het
Wdr1	T	C	5: 38,687,014 (GRCm39)		probably null	Het
Wdr31	A	G	4: 62,381,661 (GRCm39)	Y57H	probably damaging	Het
Zbtb44	T	A	9: 30,964,678 (GRCm39)	H29Q	probably damaging	Het
Zfp532	T	A	18: 65,757,509 (GRCm39)	S481T	probably damaging	Het
Zfp955b	G	T	17: 33,521,084 (GRCm39)	R184S	probably benign	Het

Other mutations in Tmem210

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02651:Tmem210	APN	2	25,179,101 (GRCm39)	missense	possibly damaging	0.71
R0135:Tmem210	UTSW	2	25,178,480 (GRCm39)	missense	probably damaging	0.99
R1758:Tmem210	UTSW	2	25,178,435 (GRCm39)	missense	probably damaging	0.96
R3837:Tmem210	UTSW	2	25,178,444 (GRCm39)	missense	possibly damaging	0.60
R3838:Tmem210	UTSW	2	25,178,444 (GRCm39)	missense	possibly damaging	0.60
R6348:Tmem210	UTSW	2	25,178,796 (GRCm39)	missense	probably benign	0.27
R6349:Tmem210	UTSW	2	25,179,048 (GRCm39)	missense	possibly damaging	0.85

Predicted Primers

PCR Primer
(F):5'- TACTGACCCTGGGCTGTTAG -3'
(R):5'- GGTCTATGCGATCTTCCTGG -3'

Sequencing Primer
(F):5'- ACCCTGGGCTGTTAGTCATGC -3'
(R):5'- GCGATCTTCCTGGACTCCGTAG -3'

Posted On

2017-10-10