Mutagenetix > Incidental Mutation

Incidental Mutation 'R5950:Ralgapa1'

472400

Institutional Source

Beutler Lab

Gene Symbol

Ralgapa1

Ensembl Gene

ENSMUSG00000021027

Gene Name

Ral GTPase activating protein, alpha subunit 1

Synonyms

Garnl1, 4930400K19Rik, Tulip1, 2310003F20Rik

MMRRC Submission

044140-MU

Accession Numbers

Essential gene?

Probably essential (E-score: 0.814) question?

Stock #

R5950 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

55649681-55867952 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 55785050 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Serine at position 737 (T737S)

Ref Sequence

ENSEMBL: ENSMUSP00000151857 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000085385] [ENSMUST00000110687] [ENSMUST00000219432] [ENSMUST00000220367] [ENSMUST00000226244]

AlphaFold

Q6GYP7

Predicted Effect

probably benign
Transcript: ENSMUST00000085385
AA Change: T737S

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000082503
Gene: ENSMUSG00000021027
AA Change: T737S

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2003	7.4e-66	PFAM

Predicted Effect

probably benign
Transcript: ENSMUST00000110687
AA Change: T737S

PolyPhen 2 Score 0.027 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000106315
Gene: ENSMUSG00000021027
AA Change: T737S

Domain	Start	End	E-Value	Type
low complexity region	644	651	N/A	INTRINSIC
low complexity region	676	690	N/A	INTRINSIC
low complexity region	692	704	N/A	INTRINSIC
low complexity region	894	915	N/A	INTRINSIC
low complexity region	1386	1395	N/A	INTRINSIC
low complexity region	1784	1798	N/A	INTRINSIC
Pfam:Rap_GAP	1824	2001	1.9e-48	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000219432
AA Change: T737S

PolyPhen 2 Score 0.585 (Sensitivity: 0.88; Specificity: 0.91)

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219529

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000219542

Predicted Effect

probably benign
Transcript: ENSMUST00000220367
AA Change: T737S

PolyPhen 2 Score 0.430 (Sensitivity: 0.89; Specificity: 0.90)

Predicted Effect

probably benign
Transcript: ENSMUST00000226244
AA Change: T737S

PolyPhen 2 Score 0.279 (Sensitivity: 0.91; Specificity: 0.88)

Meta Mutation Damage Score

0.0679

Coding Region Coverage

1x: 99.9%
3x: 99.6%
10x: 97.9%
20x: 93.6%

Validation Efficiency

93% (75/81)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9. [provided by RefSeq, Oct 2016]

Allele List at MGI

Other mutations in this stock

Total: 68 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca15	G	A	7: 119,981,879 (GRCm39)	G1065R	probably damaging	Het
Acox2	A	T	14: 8,255,793 (GRCm38)	Y113N	probably benign	Het
Arid4b	T	A	13: 14,365,849 (GRCm39)		probably benign	Het
Atf6	C	T	1: 170,662,448 (GRCm39)	G271R	probably damaging	Het
Bnip5	T	A	17: 29,124,729 (GRCm39)	Q256L	possibly damaging	Het
Cct8l1	C	A	5: 25,722,741 (GRCm39)	F485L	probably benign	Het
Cdk5r2	A	G	1: 74,894,561 (GRCm39)	E102G	probably damaging	Het
Celsr1	A	T	15: 85,916,701 (GRCm39)	V424E	probably damaging	Het
Ces1h	T	C	8: 94,089,587 (GRCm39)	T271A	probably benign	Het
Cfap44	T	C	16: 44,300,210 (GRCm39)	I1738T	probably damaging	Het
Cntnap3	G	A	13: 64,935,583 (GRCm39)	L427F	probably damaging	Het
Crybg3	T	C	16: 59,313,934 (GRCm39)		probably benign	Het
Dis3l2	A	G	1: 86,948,830 (GRCm39)	D589G	probably damaging	Het
Dlg1	A	G	16: 31,484,401 (GRCm39)	R10G	probably damaging	Het
Dnajc1	A	C	2: 18,311,752 (GRCm39)		probably benign	Het
Dpy19l2	T	A	9: 24,492,430 (GRCm39)	T723S	probably benign	Het
Dsg3	A	T	18: 20,671,586 (GRCm39)	N764Y	probably damaging	Het
Dst	G	T	1: 34,301,141 (GRCm39)	R3654L	probably damaging	Het
Dynlt5	T	G	4: 102,861,447 (GRCm39)	L147R	probably damaging	Het
Evl	C	T	12: 108,641,812 (GRCm39)	T198I	probably benign	Het
Hectd2	A	G	19: 36,574,639 (GRCm39)		probably benign	Het
Hsp90b1	A	G	10: 86,537,609 (GRCm39)	V232A	possibly damaging	Het
Igsf5	T	C	16: 96,174,072 (GRCm39)	V34A	probably benign	Het
Ikzf2	A	T	1: 69,722,403 (GRCm39)	N41K	probably damaging	Het
Kif6	G	T	17: 50,022,116 (GRCm39)	A339S	probably damaging	Het
Klhl1	T	A	14: 96,477,790 (GRCm39)	D426V	probably damaging	Het
Knop1	A	C	7: 118,452,557 (GRCm39)	V54G	probably damaging	Het
Lama4	T	A	10: 38,906,444 (GRCm39)	V270D	probably benign	Het
Lnx2	A	G	5: 146,961,160 (GRCm39)		probably null	Het
Lrp5	A	T	19: 3,652,333 (GRCm39)	V1179E	probably benign	Het
Lrpprc	T	G	17: 85,047,598 (GRCm39)	D878A	possibly damaging	Het
Lrrc14	A	T	15: 76,599,510 (GRCm39)		probably benign	Het
Ltbp1	A	T	17: 75,580,865 (GRCm39)	D660V	probably damaging	Het
Macf1	C	G	4: 123,333,229 (GRCm39)		probably null	Het
Map3k21	C	A	8: 126,668,499 (GRCm39)	T695K	possibly damaging	Het
Mcm3ap	T	A	10: 76,324,253 (GRCm39)	D895E	possibly damaging	Het
Mink1	T	A	11: 70,500,412 (GRCm39)	D779E	possibly damaging	Het
Mkrn3	T	C	7: 62,069,467 (GRCm39)	E108G	probably damaging	Het
Mtcl3	T	C	10: 29,019,644 (GRCm39)		probably benign	Het
Nars1	A	G	18: 64,643,556 (GRCm39)	V141A	possibly damaging	Het
Or8g34	T	C	9: 39,373,633 (GRCm39)	V299A	probably benign	Het
Pard3b	T	A	1: 62,255,690 (GRCm39)	Y572N	probably benign	Het
Pcnx3	A	C	19: 5,717,186 (GRCm39)	M1599R	possibly damaging	Het
Pitx2	A	G	3: 129,012,169 (GRCm39)	S180G	probably damaging	Het
Pkd2l1	A	C	19: 44,140,529 (GRCm39)	V608G	probably benign	Het
Pkhd1l1	A	G	15: 44,396,361 (GRCm39)	D1961G	probably benign	Het
Pxylp1	T	C	9: 96,721,179 (GRCm39)	T109A	probably damaging	Het
Rai1	T	A	11: 60,078,419 (GRCm39)	C828S	probably damaging	Het
Scn11a	A	G	9: 119,640,190 (GRCm39)	V235A	probably damaging	Het
Sfxn1	A	T	13: 54,245,306 (GRCm39)	T134S	probably benign	Het
Skint1	A	G	4: 111,876,532 (GRCm39)	Y151C	probably benign	Het
Slc11a1	G	T	1: 74,416,335 (GRCm39)	W54L	probably benign	Het
Slc49a3	A	T	5: 108,593,351 (GRCm39)	H162Q	probably damaging	Het
Synpo2l	T	A	14: 20,716,003 (GRCm39)	Q191L	probably benign	Het
Tank	A	G	2: 61,483,913 (GRCm39)		probably benign	Het
Terb1	A	T	8: 105,215,117 (GRCm39)		probably null	Het
Trdc	T	C	14: 54,381,768 (GRCm39)		probably benign	Het
Tsen34	A	G	7: 3,697,787 (GRCm39)	I63V	probably null	Het
Ust	T	C	10: 8,123,865 (GRCm39)	H257R	probably benign	Het
Vmn2r6	T	C	3: 64,472,652 (GRCm39)	Q23R	probably benign	Het
Wdr93	A	T	7: 79,423,179 (GRCm39)	H481L	probably damaging	Het
Xirp2	A	G	2: 67,341,664 (GRCm39)	T1302A	possibly damaging	Het
Zc3h6	C	A	2: 128,839,710 (GRCm39)	Y174*	probably null	Het
Zcchc7	T	G	4: 44,931,244 (GRCm39)	D144E	possibly damaging	Het
Zfp472	T	A	17: 33,196,481 (GRCm39)	H185Q	possibly damaging	Het
Zfp523	C	A	17: 28,421,532 (GRCm39)	P66H	probably benign	Het
Zfp712	A	T	13: 67,192,881 (GRCm39)	L57Q	probably damaging	Het
Zik1	G	A	7: 10,224,498 (GRCm39)	Q200*	probably null	Het

Other mutations in Ralgapa1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Ralgapa1	APN	12	55,769,558 (GRCm39)	missense	probably damaging	0.98
IGL00494:Ralgapa1	APN	12	55,793,970 (GRCm39)	missense	probably damaging	1.00
IGL00731:Ralgapa1	APN	12	55,749,237 (GRCm39)	missense	possibly damaging	0.94
IGL00851:Ralgapa1	APN	12	55,756,360 (GRCm39)	missense	possibly damaging	0.93
IGL01133:Ralgapa1	APN	12	55,689,144 (GRCm39)	missense	probably damaging	0.99
IGL01133:Ralgapa1	APN	12	55,689,133 (GRCm39)	missense	probably damaging	1.00
IGL01354:Ralgapa1	APN	12	55,824,101 (GRCm39)	missense	possibly damaging	0.68
IGL01514:Ralgapa1	APN	12	55,766,442 (GRCm39)	missense	probably damaging	0.97
IGL02033:Ralgapa1	APN	12	55,689,262 (GRCm39)	missense	possibly damaging	0.69
IGL02064:Ralgapa1	APN	12	55,754,862 (GRCm39)	missense	probably damaging	1.00
IGL02556:Ralgapa1	APN	12	55,689,234 (GRCm39)	missense	possibly damaging	0.80
IGL02605:Ralgapa1	APN	12	55,759,450 (GRCm39)	missense	possibly damaging	0.90
IGL02657:Ralgapa1	APN	12	55,720,292 (GRCm39)	missense	probably damaging	1.00
IGL02676:Ralgapa1	APN	12	55,723,202 (GRCm39)	missense	probably damaging	1.00
IGL02894:Ralgapa1	APN	12	55,763,854 (GRCm39)	missense	possibly damaging	0.79
IGL02944:Ralgapa1	APN	12	55,804,736 (GRCm39)	missense	probably benign	0.01
Anhydrous	UTSW	12	55,842,563 (GRCm39)	critical splice acceptor site	probably null
Aqueous	UTSW	12	55,745,639 (GRCm39)	missense	probably damaging	1.00
bantam	UTSW	12	55,769,558 (GRCm39)	critical splice donor site	probably null
Deliquescent	UTSW	12	55,829,685 (GRCm39)	splice site	probably benign
wickedwarlock	UTSW	12	55,824,077 (GRCm39)	missense	probably null	0.99
F5770:Ralgapa1	UTSW	12	55,842,438 (GRCm39)	splice site	probably benign
IGL03046:Ralgapa1	UTSW	12	55,741,942 (GRCm39)	missense	probably damaging	1.00
R0011:Ralgapa1	UTSW	12	55,833,048 (GRCm39)	missense	probably damaging	0.99
R0096:Ralgapa1	UTSW	12	55,786,290 (GRCm39)	missense	probably damaging	1.00
R0277:Ralgapa1	UTSW	12	55,724,023 (GRCm39)	missense	probably damaging	0.99
R0323:Ralgapa1	UTSW	12	55,724,023 (GRCm39)	missense	probably damaging	0.99
R0333:Ralgapa1	UTSW	12	55,829,685 (GRCm39)	splice site	probably benign
R0361:Ralgapa1	UTSW	12	55,723,354 (GRCm39)	missense	possibly damaging	0.93
R0385:Ralgapa1	UTSW	12	55,723,823 (GRCm39)	missense	probably damaging	1.00
R0386:Ralgapa1	UTSW	12	55,754,852 (GRCm39)	missense	probably benign	0.03
R0498:Ralgapa1	UTSW	12	55,736,576 (GRCm39)	missense	possibly damaging	0.66
R0552:Ralgapa1	UTSW	12	55,723,550 (GRCm39)	missense	probably benign	0.27
R0564:Ralgapa1	UTSW	12	55,829,670 (GRCm39)	missense	possibly damaging	0.84
R0611:Ralgapa1	UTSW	12	55,842,483 (GRCm39)	missense	probably damaging	0.99
R0730:Ralgapa1	UTSW	12	55,712,448 (GRCm39)	missense	probably damaging	1.00
R0741:Ralgapa1	UTSW	12	55,723,366 (GRCm39)	missense	probably damaging	0.99
R0815:Ralgapa1	UTSW	12	55,829,562 (GRCm39)	splice site	probably benign
R0815:Ralgapa1	UTSW	12	55,809,466 (GRCm39)	nonsense	probably null
R0863:Ralgapa1	UTSW	12	55,829,562 (GRCm39)	splice site	probably benign
R0863:Ralgapa1	UTSW	12	55,809,466 (GRCm39)	nonsense	probably null
R1068:Ralgapa1	UTSW	12	55,837,095 (GRCm39)	critical splice donor site	probably null
R1147:Ralgapa1	UTSW	12	55,749,265 (GRCm39)	missense	probably damaging	1.00
R1147:Ralgapa1	UTSW	12	55,749,265 (GRCm39)	missense	probably damaging	1.00
R1256:Ralgapa1	UTSW	12	55,809,446 (GRCm39)	missense	possibly damaging	0.94
R1343:Ralgapa1	UTSW	12	55,754,763 (GRCm39)	missense	probably damaging	1.00
R1378:Ralgapa1	UTSW	12	55,723,711 (GRCm39)	missense	probably damaging	1.00
R1474:Ralgapa1	UTSW	12	55,788,265 (GRCm39)	missense	probably benign	0.09
R1494:Ralgapa1	UTSW	12	55,731,309 (GRCm39)	missense	probably damaging	0.99
R1593:Ralgapa1	UTSW	12	55,817,488 (GRCm39)	missense	probably damaging	1.00
R1607:Ralgapa1	UTSW	12	55,788,321 (GRCm39)	missense	probably damaging	1.00
R1681:Ralgapa1	UTSW	12	55,809,388 (GRCm39)	missense	probably benign	0.35
R1689:Ralgapa1	UTSW	12	55,723,552 (GRCm39)	missense	possibly damaging	0.79
R1714:Ralgapa1	UTSW	12	55,689,174 (GRCm39)	missense	probably damaging	1.00
R1832:Ralgapa1	UTSW	12	55,804,752 (GRCm39)	missense	probably benign	0.03
R1870:Ralgapa1	UTSW	12	55,723,817 (GRCm39)	missense	possibly damaging	0.66
R2040:Ralgapa1	UTSW	12	55,833,107 (GRCm39)	missense	probably damaging	1.00
R2043:Ralgapa1	UTSW	12	55,723,811 (GRCm39)	missense	probably damaging	0.99
R2046:Ralgapa1	UTSW	12	55,741,945 (GRCm39)	missense	probably damaging	1.00
R2109:Ralgapa1	UTSW	12	55,822,973 (GRCm39)	missense	possibly damaging	0.90
R2114:Ralgapa1	UTSW	12	55,833,134 (GRCm39)	critical splice acceptor site	probably null
R2115:Ralgapa1	UTSW	12	55,833,134 (GRCm39)	critical splice acceptor site	probably null
R2202:Ralgapa1	UTSW	12	55,659,585 (GRCm39)	splice site	probably null
R2203:Ralgapa1	UTSW	12	55,659,585 (GRCm39)	splice site	probably null
R2233:Ralgapa1	UTSW	12	55,763,856 (GRCm39)	missense	probably benign	0.13
R2235:Ralgapa1	UTSW	12	55,763,856 (GRCm39)	missense	probably benign	0.13
R2341:Ralgapa1	UTSW	12	55,723,909 (GRCm39)	missense	possibly damaging	0.66
R2507:Ralgapa1	UTSW	12	55,764,986 (GRCm39)	missense	probably damaging	1.00
R2508:Ralgapa1	UTSW	12	55,764,986 (GRCm39)	missense	probably damaging	1.00
R2972:Ralgapa1	UTSW	12	55,867,540 (GRCm39)	missense	possibly damaging	0.61
R3160:Ralgapa1	UTSW	12	55,756,371 (GRCm39)	missense	probably damaging	1.00
R3162:Ralgapa1	UTSW	12	55,756,371 (GRCm39)	missense	probably damaging	1.00
R3401:Ralgapa1	UTSW	12	55,705,922 (GRCm39)	missense	possibly damaging	0.66
R3416:Ralgapa1	UTSW	12	55,817,398 (GRCm39)	splice site	probably benign
R3499:Ralgapa1	UTSW	12	55,741,928 (GRCm39)	splice site	probably benign
R3799:Ralgapa1	UTSW	12	55,705,915 (GRCm39)	missense	probably damaging	1.00
R3948:Ralgapa1	UTSW	12	55,745,552 (GRCm39)	missense	probably damaging	1.00
R4039:Ralgapa1	UTSW	12	55,842,486 (GRCm39)	missense	probably damaging	0.99
R4120:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4165:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4166:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4212:Ralgapa1	UTSW	12	55,786,115 (GRCm39)	critical splice donor site	probably null
R4232:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4233:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4234:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4235:Ralgapa1	UTSW	12	55,687,429 (GRCm39)	missense	probably damaging	1.00
R4399:Ralgapa1	UTSW	12	55,842,563 (GRCm39)	critical splice acceptor site	probably null
R4698:Ralgapa1	UTSW	12	55,724,061 (GRCm39)	splice site	probably null
R4715:Ralgapa1	UTSW	12	55,740,243 (GRCm39)	missense	probably damaging	1.00
R4755:Ralgapa1	UTSW	12	55,759,533 (GRCm39)	missense	probably damaging	1.00
R4810:Ralgapa1	UTSW	12	55,841,778 (GRCm39)	critical splice donor site	probably null
R4827:Ralgapa1	UTSW	12	55,723,222 (GRCm39)	missense	probably damaging	1.00
R4849:Ralgapa1	UTSW	12	55,745,588 (GRCm39)	missense	probably damaging	0.99
R4934:Ralgapa1	UTSW	12	55,809,359 (GRCm39)	missense	possibly damaging	0.94
R5006:Ralgapa1	UTSW	12	55,764,899 (GRCm39)	missense	probably benign	0.02
R5114:Ralgapa1	UTSW	12	55,659,508 (GRCm39)	missense	possibly damaging	0.84
R5140:Ralgapa1	UTSW	12	55,712,459 (GRCm39)	missense	probably damaging	1.00
R5140:Ralgapa1	UTSW	12	55,822,937 (GRCm39)	missense	probably damaging	1.00
R5168:Ralgapa1	UTSW	12	55,804,817 (GRCm39)	missense	probably benign	0.05
R5407:Ralgapa1	UTSW	12	55,723,582 (GRCm39)	missense	possibly damaging	0.93
R5441:Ralgapa1	UTSW	12	55,766,408 (GRCm39)	missense	probably damaging	1.00
R5473:Ralgapa1	UTSW	12	55,723,495 (GRCm39)	missense	probably benign	0.41
R5624:Ralgapa1	UTSW	12	55,659,523 (GRCm39)	missense	probably damaging	1.00
R5766:Ralgapa1	UTSW	12	55,867,551 (GRCm39)	start codon destroyed	probably null	0.99
R5826:Ralgapa1	UTSW	12	55,723,898 (GRCm39)	missense	probably damaging	1.00
R5980:Ralgapa1	UTSW	12	55,817,401 (GRCm39)	splice site	probably null
R6019:Ralgapa1	UTSW	12	55,730,827 (GRCm39)	missense	possibly damaging	0.92
R6065:Ralgapa1	UTSW	12	55,804,709 (GRCm39)	critical splice donor site	probably null
R6326:Ralgapa1	UTSW	12	55,793,931 (GRCm39)	missense	probably damaging	1.00
R6355:Ralgapa1	UTSW	12	55,745,639 (GRCm39)	missense	probably damaging	1.00
R6408:Ralgapa1	UTSW	12	55,730,695 (GRCm39)	nonsense	probably null
R6448:Ralgapa1	UTSW	12	55,766,446 (GRCm39)	missense	probably benign	0.14
R6453:Ralgapa1	UTSW	12	55,785,104 (GRCm39)	missense	probably damaging	1.00
R6590:Ralgapa1	UTSW	12	55,769,558 (GRCm39)	critical splice donor site	probably null
R6690:Ralgapa1	UTSW	12	55,769,558 (GRCm39)	critical splice donor site	probably null
R6738:Ralgapa1	UTSW	12	55,809,512 (GRCm39)	missense	probably damaging	1.00
R6836:Ralgapa1	UTSW	12	55,651,058 (GRCm39)	splice site	probably null
R6936:Ralgapa1	UTSW	12	55,832,997 (GRCm39)	missense	probably damaging	0.99
R6945:Ralgapa1	UTSW	12	55,822,976 (GRCm39)	missense	possibly damaging	0.64
R7028:Ralgapa1	UTSW	12	55,804,844 (GRCm39)	missense	probably damaging	1.00
R7075:Ralgapa1	UTSW	12	55,867,508 (GRCm39)	missense	possibly damaging	0.66
R7076:Ralgapa1	UTSW	12	55,768,361 (GRCm39)	missense	possibly damaging	0.82
R7098:Ralgapa1	UTSW	12	55,837,095 (GRCm39)	critical splice donor site	probably null
R7231:Ralgapa1	UTSW	12	55,650,976 (GRCm39)	missense	probably damaging	1.00
R7254:Ralgapa1	UTSW	12	55,741,978 (GRCm39)	missense	probably damaging	1.00
R7326:Ralgapa1	UTSW	12	55,755,789 (GRCm39)	missense	probably damaging	1.00
R7485:Ralgapa1	UTSW	12	55,759,457 (GRCm39)	missense	probably damaging	1.00
R7580:Ralgapa1	UTSW	12	55,765,013 (GRCm39)	missense	probably benign	0.00
R7677:Ralgapa1	UTSW	12	55,705,928 (GRCm39)	missense	probably damaging	0.96
R7702:Ralgapa1	UTSW	12	55,756,341 (GRCm39)	missense	probably damaging	1.00
R7702:Ralgapa1	UTSW	12	55,756,340 (GRCm39)	missense	probably damaging	1.00
R7707:Ralgapa1	UTSW	12	55,824,077 (GRCm39)	missense	probably null	0.99
R7723:Ralgapa1	UTSW	12	55,788,298 (GRCm39)	missense	probably benign
R7763:Ralgapa1	UTSW	12	55,804,740 (GRCm39)	missense	probably benign	0.28
R7791:Ralgapa1	UTSW	12	55,788,304 (GRCm39)	missense	probably damaging	0.97
R7812:Ralgapa1	UTSW	12	55,766,413 (GRCm39)	missense	possibly damaging	0.67
R7868:Ralgapa1	UTSW	12	55,659,423 (GRCm39)	missense	probably benign	0.00
R7895:Ralgapa1	UTSW	12	55,793,934 (GRCm39)	missense	probably benign	0.44
R7896:Ralgapa1	UTSW	12	55,744,663 (GRCm39)	missense	probably benign	0.01
R8004:Ralgapa1	UTSW	12	55,749,242 (GRCm39)	missense	probably damaging	0.99
R8094:Ralgapa1	UTSW	12	55,829,631 (GRCm39)	missense	probably damaging	0.99
R8213:Ralgapa1	UTSW	12	55,769,699 (GRCm39)	missense	probably damaging	0.99
R8307:Ralgapa1	UTSW	12	55,788,308 (GRCm39)	missense	probably damaging	0.99
R8423:Ralgapa1	UTSW	12	55,705,847 (GRCm39)	missense	probably damaging	0.99
R8462:Ralgapa1	UTSW	12	55,723,303 (GRCm39)	missense	possibly damaging	0.90
R8469:Ralgapa1	UTSW	12	55,786,198 (GRCm39)	missense	probably damaging	1.00
R8675:Ralgapa1	UTSW	12	55,785,002 (GRCm39)	missense	possibly damaging	0.93
R8802:Ralgapa1	UTSW	12	55,785,101 (GRCm39)	missense	probably damaging	0.99
R8937:Ralgapa1	UTSW	12	55,749,345 (GRCm39)	missense	probably damaging	0.96
R8953:Ralgapa1	UTSW	12	55,867,546 (GRCm39)	missense	probably damaging	0.99
R8974:Ralgapa1	UTSW	12	55,723,791 (GRCm39)	missense	probably benign
R9011:Ralgapa1	UTSW	12	55,652,314 (GRCm39)	intron	probably benign
R9089:Ralgapa1	UTSW	12	55,723,351 (GRCm39)	missense	probably damaging	0.97
R9124:Ralgapa1	UTSW	12	55,781,881 (GRCm39)	missense	probably damaging	1.00
R9254:Ralgapa1	UTSW	12	55,769,583 (GRCm39)	missense	probably damaging	1.00
R9320:Ralgapa1	UTSW	12	55,755,843 (GRCm39)	missense	possibly damaging	0.59
R9379:Ralgapa1	UTSW	12	55,769,583 (GRCm39)	missense	probably damaging	1.00
R9446:Ralgapa1	UTSW	12	55,754,808 (GRCm39)	missense	probably damaging	0.97
R9684:Ralgapa1	UTSW	12	55,659,485 (GRCm39)	missense	possibly damaging	0.63
Z1176:Ralgapa1	UTSW	12	55,755,865 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATTGGGCTATAAGGATTACTGAGG -3'
(R):5'- CTTTATAATGCACTCCGAGGAAGC -3'

Sequencing Primer
(F):5'- TTACTGAGGAAGGGGAGGAAGTTTTC -3'
(R):5'- TATAATGCACTCCGAGGAAGCCTTAG -3'

Posted On

2017-03-31