Mutagenetix > Incidental Mutation

Incidental Mutation 'R0511:Col4a1'

46913

Institutional Source

Beutler Lab

Gene Symbol

Col4a1

Ensembl Gene

ENSMUSG00000031502

Gene Name

collagen, type IV, alpha 1

Synonyms

Del(8)Bru44H, Del(8)44H, alpha1(IV) collagen, Col4a-1, Bru, Svc, Raw

MMRRC Submission

038705-MU

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R0511 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

11248423-11362826 bp(-) (GRCm39)

Type of Mutation

critical splice acceptor site

DNA Base Change (assembly)

T to C at 11258333 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Gene Model

predicted gene model for transcript(s): [ENSMUST00000033898] [ENSMUST00000033898] [ENSMUST00000209735] [ENSMUST00000209735]

AlphaFold

P02463

Predicted Effect

probably null
Transcript: ENSMUST00000033898

SMART Domains

Protein: ENSMUSP00000033898
Gene: ENSMUSG00000031502

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
low complexity region	28	43	N/A	INTRINSIC
internal_repeat_2	49	89	2.1e-8	PROSPERO
Pfam:Collagen	103	163	6.1e-11	PFAM
Pfam:Collagen	167	225	7.8e-10	PFAM
low complexity region	232	248	N/A	INTRINSIC
Pfam:Collagen	274	334	1.7e-11	PFAM
low complexity region	356	389	N/A	INTRINSIC
low complexity region	404	426	N/A	INTRINSIC
low complexity region	435	455	N/A	INTRINSIC
Pfam:Collagen	472	533	7.3e-12	PFAM
Pfam:Collagen	539	597	4.8e-9	PFAM
low complexity region	600	636	N/A	INTRINSIC
Pfam:Collagen	642	689	4.5e-8	PFAM
Pfam:Collagen	689	746	3.5e-8	PFAM
Pfam:Collagen	736	800	2.2e-9	PFAM
Pfam:Collagen	837	896	5.2e-11	PFAM
Pfam:Collagen	882	940	1.9e-10	PFAM
Pfam:Collagen	943	1007	1.7e-10	PFAM
Pfam:Collagen	996	1058	2e-9	PFAM
Pfam:Collagen	1057	1121	1.5e-10	PFAM
low complexity region	1133	1148	N/A	INTRINSIC
Pfam:Collagen	1174	1233	8.6e-11	PFAM
low complexity region	1236	1266	N/A	INTRINSIC
Pfam:Collagen	1269	1337	1e-8	PFAM
Pfam:Collagen	1290	1354	2.2e-9	PFAM
Pfam:Collagen	1384	1443	1e-10	PFAM
C4	1445	1554	3.49e-65	SMART
C4	1555	1668	1.53e-79	SMART

Predicted Effect

probably null
Transcript: ENSMUST00000033898

SMART Domains

Protein: ENSMUSP00000033898
Gene: ENSMUSG00000031502

Domain	Start	End	E-Value	Type
signal peptide	1	25	N/A	INTRINSIC
low complexity region	28	43	N/A	INTRINSIC
internal_repeat_2	49	89	2.1e-8	PROSPERO
Pfam:Collagen	103	163	6.1e-11	PFAM
Pfam:Collagen	167	225	7.8e-10	PFAM
low complexity region	232	248	N/A	INTRINSIC
Pfam:Collagen	274	334	1.7e-11	PFAM
low complexity region	356	389	N/A	INTRINSIC
low complexity region	404	426	N/A	INTRINSIC
low complexity region	435	455	N/A	INTRINSIC
Pfam:Collagen	472	533	7.3e-12	PFAM
Pfam:Collagen	539	597	4.8e-9	PFAM
low complexity region	600	636	N/A	INTRINSIC
Pfam:Collagen	642	689	4.5e-8	PFAM
Pfam:Collagen	689	746	3.5e-8	PFAM
Pfam:Collagen	736	800	2.2e-9	PFAM
Pfam:Collagen	837	896	5.2e-11	PFAM
Pfam:Collagen	882	940	1.9e-10	PFAM
Pfam:Collagen	943	1007	1.7e-10	PFAM
Pfam:Collagen	996	1058	2e-9	PFAM
Pfam:Collagen	1057	1121	1.5e-10	PFAM
low complexity region	1133	1148	N/A	INTRINSIC
Pfam:Collagen	1174	1233	8.6e-11	PFAM
low complexity region	1236	1266	N/A	INTRINSIC
Pfam:Collagen	1269	1337	1e-8	PFAM
Pfam:Collagen	1290	1354	2.2e-9	PFAM
Pfam:Collagen	1384	1443	1e-10	PFAM
C4	1445	1554	3.49e-65	SMART
C4	1555	1668	1.53e-79	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000130488

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000151014

Predicted Effect

probably null
Transcript: ENSMUST00000209598

Predicted Effect

probably null
Transcript: ENSMUST00000209598

Predicted Effect

probably null
Transcript: ENSMUST00000209598

Predicted Effect

probably null
Transcript: ENSMUST00000209598

Predicted Effect

probably benign
Transcript: ENSMUST00000209735

Predicted Effect

probably benign
Transcript: ENSMUST00000209735

Meta Mutation Damage Score

0.9560

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.7%
20x: 93.8%

Validation Efficiency

100% (116/116)

MGI Phenotype

FUNCTION: This gene encodes the alpha-1 subunit of the type IV collagens, an essential component of basement membranes. The encoded protein forms a triple helical heterotrimer comprised of two alpha-1 and one alpha-2 subunits that assembles into a type IV collagen network. This gene is located adjacent to the gene encoding alpha-2 subunit. Mice lacking both the alpha-1 and alpha-2 subunits of collagen IV die in utero due to structural deficiencies in the basement membranes and certain mutations in this gene cause perinatal cerebral hemorrhage and porencephaly. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Nov 2015]
PHENOTYPE: Mice with ENU induced alleles have various eye and vision defects and may show bruising at birth. Mice carrying the G498V mutation have renal glomerular defects that resolve within the first weeks of life, but show retinal tortuosity, muscular dystrophy, brain hemorrhages, and renal cysts as adults. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 118 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Aadacl2fm1	A	G	3: 59,843,771 (GRCm39)	H155R	possibly damaging	Het
Abca13	G	T	11: 9,244,559 (GRCm39)	V2141L	probably benign	Het
Adam17	T	C	12: 21,390,459 (GRCm39)		probably benign	Het
Adam3	A	T	8: 25,185,331 (GRCm39)	C456S	probably damaging	Het
Aldh4a1	G	T	4: 139,369,882 (GRCm39)		probably benign	Het
Anapc4	A	G	5: 52,999,359 (GRCm39)		probably benign	Het
Ank3	A	T	10: 69,718,198 (GRCm39)	Q483L	probably damaging	Het
Ankle2	A	G	5: 110,389,925 (GRCm39)		probably benign	Het
Ankrd13b	T	A	11: 77,364,114 (GRCm39)	T150S	possibly damaging	Het
Apeh	A	G	9: 107,964,254 (GRCm39)	M524T	probably benign	Het
Arl14epl	T	A	18: 47,059,484 (GRCm39)		probably null	Het
Atg2a	T	C	19: 6,302,569 (GRCm39)	F964S	possibly damaging	Het
Atg2b	C	T	12: 105,583,412 (GRCm39)	V2050M	probably damaging	Het
Atp2b4	A	T	1: 133,659,956 (GRCm39)		probably benign	Het
Bbof1	T	A	12: 84,477,045 (GRCm39)	S512T	probably benign	Het
Camta1	C	A	4: 151,159,597 (GRCm39)	R1614L	probably damaging	Het
Car10	T	C	11: 93,381,408 (GRCm39)	Y100H	probably damaging	Het
Ccdc81	T	A	7: 89,542,504 (GRCm39)	E124V	probably damaging	Het
Cd84	A	G	1: 171,700,494 (GRCm39)	T204A	probably benign	Het
Celf2	A	G	2: 6,608,987 (GRCm39)	S178P	probably damaging	Het
Chat	G	A	14: 32,130,976 (GRCm39)	T555M	probably damaging	Het
Chd6	A	G	2: 160,834,111 (GRCm39)	F917S	probably damaging	Het
Chrna2	C	A	14: 66,386,553 (GRCm39)	T233N	probably damaging	Het
Cnpy2	T	C	10: 128,162,054 (GRCm39)	V109A	probably benign	Het
Csmd1	C	T	8: 15,982,529 (GRCm39)	V2713M	possibly damaging	Het
Cuedc1	G	A	11: 88,074,231 (GRCm39)	R255Q	probably damaging	Het
Cxcl15	A	T	5: 90,945,897 (GRCm39)		probably benign	Het
Dach1	A	T	14: 98,138,765 (GRCm39)	H559Q	possibly damaging	Het
Dele1	T	C	18: 38,387,124 (GRCm39)		probably null	Het
Dennd4c	C	T	4: 86,744,259 (GRCm39)	T1367M	probably damaging	Het
Depdc5	T	A	5: 33,102,372 (GRCm39)	Y365*	probably null	Het
Dicer1	T	C	12: 104,669,100 (GRCm39)	Y1194C	possibly damaging	Het
Dmxl1	C	G	18: 50,024,534 (GRCm39)	S1736*	probably null	Het
Dnah7a	C	T	1: 53,536,285 (GRCm39)	R2586K	probably benign	Het
Dnajb8	T	C	6: 88,199,467 (GRCm39)	M1T	probably null	Het
Dync2h1	G	A	9: 7,122,692 (GRCm39)	P2088L	probably benign	Het
Eftud2	T	G	11: 102,735,048 (GRCm39)	H617P	probably damaging	Het
Ephb1	A	G	9: 101,873,179 (GRCm39)		probably benign	Het
Fam184a	G	T	10: 53,574,975 (GRCm39)	H155Q	probably benign	Het
Firrm	T	C	1: 163,799,412 (GRCm39)		probably null	Het
Ganc	G	T	2: 120,278,882 (GRCm39)	E700*	probably null	Het
Gm10912	A	G	2: 103,897,290 (GRCm39)		probably benign	Het
Haus5	A	T	7: 30,358,492 (GRCm39)	I294N	probably damaging	Het
Hmgcr	G	T	13: 96,796,651 (GRCm39)		probably null	Het
Hr	T	A	14: 70,799,352 (GRCm39)	C641*	probably null	Het
Itga10	A	G	3: 96,565,490 (GRCm39)	N1038S	probably damaging	Het
Itgb1bp1	T	G	12: 21,321,436 (GRCm39)	Y172S	probably damaging	Het
Kprp	T	C	3: 92,732,030 (GRCm39)	N340S	probably damaging	Het
Kremen1	A	G	11: 5,165,447 (GRCm39)	I41T	probably damaging	Het
Krt6b	A	G	15: 101,586,042 (GRCm39)		probably benign	Het
Krt81	C	A	15: 101,361,508 (GRCm39)	R24L	possibly damaging	Het
Ldhd	A	G	8: 112,356,309 (GRCm39)	Y86H	probably benign	Het
Lilra6	A	T	7: 3,915,784 (GRCm39)	I76N	possibly damaging	Het
Mak	T	C	13: 41,199,743 (GRCm39)	T299A	probably benign	Het
Med25	A	G	7: 44,534,502 (GRCm39)		probably null	Het
Mpg	A	T	11: 32,180,039 (GRCm39)	N189I	probably damaging	Het
Mroh8	A	G	2: 157,071,838 (GRCm39)	Y556H	probably damaging	Het
Myh8	T	A	11: 67,175,333 (GRCm39)	S294T	probably benign	Het
Myom1	T	A	17: 71,391,312 (GRCm39)	D842E	probably benign	Het
Myorg	A	G	4: 41,498,538 (GRCm39)	F364S	probably damaging	Het
Nat2	C	T	8: 67,953,982 (GRCm39)	Q31*	probably null	Het
Nf1	T	A	11: 79,329,595 (GRCm39)	M653K	probably benign	Het
Nhs	C	A	X: 160,620,355 (GRCm39)	R1467I	probably damaging	Het
Npr2	A	G	4: 43,632,801 (GRCm39)	E206G	probably benign	Het
Nsd3	G	A	8: 26,168,732 (GRCm39)	G629D	possibly damaging	Het
Nwd1	G	A	8: 73,408,633 (GRCm39)	C831Y	probably damaging	Het
Or52r1c	T	C	7: 102,735,058 (GRCm39)	I111T	probably damaging	Het
Or5t9	A	G	2: 86,659,950 (GRCm39)	I285V	probably benign	Het
P2ry14	A	G	3: 59,023,449 (GRCm39)	S4P	possibly damaging	Het
Parp4	A	G	14: 56,873,172 (GRCm39)		probably benign	Het
Pate14	A	T	9: 36,549,873 (GRCm39)		probably null	Het
Pclo	A	G	5: 14,728,299 (GRCm39)		probably benign	Het
Pclo	T	C	5: 14,729,412 (GRCm39)		probably benign	Het
Pcnt	A	T	10: 76,240,429 (GRCm39)	S1202T	possibly damaging	Het
Pfkfb4	A	G	9: 108,856,825 (GRCm39)	Y412C	probably damaging	Het
Pgm2	T	A	5: 64,267,898 (GRCm39)	V449D	probably damaging	Het
Poldip3	T	A	15: 83,022,436 (GRCm39)	D116V	probably damaging	Het
Pom121	G	T	5: 135,410,686 (GRCm39)	Q824K	unknown	Het
Prkdc	G	T	16: 15,649,146 (GRCm39)	G3707*	probably null	Het
Prr14l	T	C	5: 33,001,560 (GRCm39)		probably benign	Het
Ptbp2	T	G	3: 119,514,613 (GRCm39)	I405L	probably benign	Het
Rad21l	A	T	2: 151,490,989 (GRCm39)		probably benign	Het
Rbm6	G	A	9: 107,724,488 (GRCm39)	Q488*	probably null	Het
Rdh1	T	A	10: 127,600,652 (GRCm39)	M225K	probably benign	Het
Recql5	T	C	11: 115,819,209 (GRCm39)	D119G	probably benign	Het
Rif1	GCCACCA	GCCA	2: 52,000,336 (GRCm39)		probably benign	Het
Robo1	T	C	16: 72,810,013 (GRCm39)		probably null	Het
Samd12	G	A	15: 53,723,567 (GRCm39)	T42I	probably benign	Het
Scn10a	A	T	9: 119,442,766 (GRCm39)	M1494K	probably damaging	Het
Sec31a	G	A	5: 100,523,099 (GRCm39)	P864L	probably benign	Het
Senp2	T	C	16: 21,855,320 (GRCm39)	V344A	probably benign	Het
Serpina5	G	A	12: 104,069,621 (GRCm39)	D278N	probably benign	Het
Sh3tc1	A	T	5: 35,860,806 (GRCm39)	V1017D	probably damaging	Het
Sin3a	T	A	9: 57,004,179 (GRCm39)	Y310*	probably null	Het
Slc25a32	T	C	15: 38,960,940 (GRCm39)	T248A	probably benign	Het
Slc35e1	T	C	8: 73,246,415 (GRCm39)		probably benign	Het
Slc4a10	G	A	2: 62,117,206 (GRCm39)	V722M	probably damaging	Het
Slco1a4	A	G	6: 141,776,586 (GRCm39)		probably benign	Het
Smg6	T	A	11: 74,819,884 (GRCm39)	Y52N	probably damaging	Het
Sncb	T	G	13: 54,913,400 (GRCm39)	T33P	probably damaging	Het
Spef2	A	G	15: 9,584,070 (GRCm39)		probably null	Het
Spmip1	G	T	6: 29,478,169 (GRCm39)		probably benign	Het
Sugp1	A	G	8: 70,512,013 (GRCm39)	E203G	probably damaging	Het
Suv39h2	A	T	2: 3,473,616 (GRCm39)	C105S	probably damaging	Het
Tlr1	A	T	5: 65,083,963 (GRCm39)	F205I	probably damaging	Het
Tnip1	A	T	11: 54,808,699 (GRCm39)	M496K	probably damaging	Het
Tnxb	G	A	17: 34,937,219 (GRCm39)	E2889K	probably damaging	Het
Trim30b	T	A	7: 104,015,010 (GRCm39)	H126L	possibly damaging	Het
Trpm7	A	T	2: 126,668,638 (GRCm39)	Y759*	probably null	Het
Ttc17	A	G	2: 94,153,465 (GRCm39)	I1000T	possibly damaging	Het
Ttc27	A	T	17: 75,025,710 (GRCm39)	N61I	probably benign	Het
Uba6	T	C	5: 86,260,609 (GRCm39)	Y990C	probably damaging	Het
Vav3	A	G	3: 109,571,756 (GRCm39)		probably benign	Het
Vmn2r55	C	T	7: 12,404,945 (GRCm39)	A153T	possibly damaging	Het
Wars2	A	G	3: 99,123,865 (GRCm39)	D242G	probably damaging	Het
Xylt2	G	A	11: 94,560,762 (GRCm39)	Q259*	probably null	Het
Zfp27	G	A	7: 29,593,947 (GRCm39)	P673S	probably damaging	Het
Zgrf1	T	C	3: 127,378,309 (GRCm39)	I1023T	possibly damaging	Het

Other mutations in Col4a1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00336:Col4a1	APN	8	11,290,077 (GRCm39)	splice site	probably benign
IGL00503:Col4a1	APN	8	11,290,076 (GRCm39)	splice site	probably benign
IGL00938:Col4a1	APN	8	11,286,456 (GRCm39)	intron	probably benign
IGL01295:Col4a1	APN	8	11,286,075 (GRCm39)	intron	probably benign
IGL01406:Col4a1	APN	8	11,268,898 (GRCm39)	missense	probably damaging	1.00
IGL01807:Col4a1	APN	8	11,297,056 (GRCm39)	utr 5 prime	probably benign
IGL01865:Col4a1	APN	8	11,251,790 (GRCm39)	utr 3 prime	probably benign
IGL02166:Col4a1	APN	8	11,294,509 (GRCm39)	unclassified	probably benign
IGL02234:Col4a1	APN	8	11,266,713 (GRCm39)	missense	probably damaging	1.00
IGL02445:Col4a1	APN	8	11,283,911 (GRCm39)	intron	probably benign
IGL02719:Col4a1	APN	8	11,281,950 (GRCm39)	intron	probably benign
IGL02817:Col4a1	APN	8	11,270,259 (GRCm39)	missense	probably damaging	1.00
IGL02821:Col4a1	APN	8	11,271,375 (GRCm39)	missense	probably benign	0.04
IGL02870:Col4a1	APN	8	11,271,375 (GRCm39)	missense	probably benign	0.04
IGL02935:Col4a1	APN	8	11,269,166 (GRCm39)	missense	probably damaging	1.00
IGL03085:Col4a1	APN	8	11,272,198 (GRCm39)	nonsense	probably null
Wayne	UTSW	8	11,259,650 (GRCm39)	missense	probably damaging	1.00
IGL03134:Col4a1	UTSW	8	11,290,069 (GRCm39)	critical splice acceptor site	probably null
R0076:Col4a1	UTSW	8	11,268,713 (GRCm39)	missense	probably damaging	1.00
R0076:Col4a1	UTSW	8	11,268,713 (GRCm39)	missense	probably damaging	1.00
R0238:Col4a1	UTSW	8	11,268,780 (GRCm39)	splice site	probably benign
R0239:Col4a1	UTSW	8	11,268,780 (GRCm39)	splice site	probably benign
R0268:Col4a1	UTSW	8	11,317,588 (GRCm39)	splice site	probably benign
R0320:Col4a1	UTSW	8	11,292,782 (GRCm39)	splice site	probably null
R0402:Col4a1	UTSW	8	11,249,838 (GRCm39)	utr 3 prime	probably benign
R0483:Col4a1	UTSW	8	11,286,423 (GRCm39)	splice site	probably benign
R0544:Col4a1	UTSW	8	11,276,487 (GRCm39)	intron	probably benign
R0630:Col4a1	UTSW	8	11,249,889 (GRCm39)	splice site	probably benign
R0648:Col4a1	UTSW	8	11,296,892 (GRCm39)	missense	unknown
R0733:Col4a1	UTSW	8	11,268,934 (GRCm39)	missense	possibly damaging	0.46
R0839:Col4a1	UTSW	8	11,271,015 (GRCm39)	missense	probably damaging	0.96
R0900:Col4a1	UTSW	8	11,268,014 (GRCm39)	small deletion	probably benign
R0941:Col4a1	UTSW	8	11,258,296 (GRCm39)	missense	unknown
R1456:Col4a1	UTSW	8	11,292,829 (GRCm39)	splice site	probably benign
R1728:Col4a1	UTSW	8	11,262,712 (GRCm39)	missense	possibly damaging	0.81
R1832:Col4a1	UTSW	8	11,264,644 (GRCm39)	splice site	probably benign
R1862:Col4a1	UTSW	8	11,276,439 (GRCm39)	intron	probably benign
R1955:Col4a1	UTSW	8	11,258,228 (GRCm39)	splice site	probably null
R2058:Col4a1	UTSW	8	11,260,792 (GRCm39)	missense	probably damaging	0.96
R2263:Col4a1	UTSW	8	11,362,586 (GRCm39)	unclassified	probably benign
R2696:Col4a1	UTSW	8	11,285,092 (GRCm39)	splice site	probably null
R3826:Col4a1	UTSW	8	11,259,650 (GRCm39)	missense	probably damaging	1.00
R3828:Col4a1	UTSW	8	11,259,650 (GRCm39)	missense	probably damaging	1.00
R3829:Col4a1	UTSW	8	11,259,650 (GRCm39)	missense	probably damaging	1.00
R3830:Col4a1	UTSW	8	11,259,650 (GRCm39)	missense	probably damaging	1.00
R3923:Col4a1	UTSW	8	11,251,665 (GRCm39)	utr 3 prime	probably benign
R3980:Col4a1	UTSW	8	11,289,155 (GRCm39)	intron	probably benign
R4120:Col4a1	UTSW	8	11,256,263 (GRCm39)	missense	unknown
R4152:Col4a1	UTSW	8	11,267,227 (GRCm39)	splice site	probably null
R4437:Col4a1	UTSW	8	11,256,387 (GRCm39)	nonsense	probably null
R5237:Col4a1	UTSW	8	11,295,068 (GRCm39)	unclassified	probably benign
R5362:Col4a1	UTSW	8	11,295,760 (GRCm39)	unclassified	probably benign
R5488:Col4a1	UTSW	8	11,362,550 (GRCm39)	unclassified	probably benign
R5489:Col4a1	UTSW	8	11,362,550 (GRCm39)	unclassified	probably benign
R5864:Col4a1	UTSW	8	11,252,973 (GRCm39)	utr 3 prime	probably benign
R5929:Col4a1	UTSW	8	11,266,788 (GRCm39)	missense	probably benign	0.17
R6159:Col4a1	UTSW	8	11,270,007 (GRCm39)	missense	probably damaging	1.00
R6261:Col4a1	UTSW	8	11,257,409 (GRCm39)	splice site	probably null
R6404:Col4a1	UTSW	8	11,257,409 (GRCm39)	splice site	probably null
R6520:Col4a1	UTSW	8	11,269,152 (GRCm39)	missense	probably damaging	1.00
R6862:Col4a1	UTSW	8	11,252,926 (GRCm39)	utr 3 prime	probably benign
R6974:Col4a1	UTSW	8	11,362,538 (GRCm39)	unclassified	probably benign
R7329:Col4a1	UTSW	8	11,276,494 (GRCm39)	critical splice acceptor site	probably null
R7893:Col4a1	UTSW	8	11,270,243 (GRCm39)	missense	unknown
R8392:Col4a1	UTSW	8	11,258,333 (GRCm39)	critical splice acceptor site	probably null
R8809:Col4a1	UTSW	8	11,295,916 (GRCm39)	missense	unknown
R8957:Col4a1	UTSW	8	11,295,906 (GRCm39)	unclassified	probably benign
R9013:Col4a1	UTSW	8	11,272,270 (GRCm39)	missense	probably benign	0.02
R9048:Col4a1	UTSW	8	11,281,944 (GRCm39)	splice site	probably benign
R9102:Col4a1	UTSW	8	11,253,007 (GRCm39)	missense	possibly damaging	0.67
R9154:Col4a1	UTSW	8	11,267,446 (GRCm39)	missense	probably damaging	0.99
R9379:Col4a1	UTSW	8	11,249,838 (GRCm39)	missense	unknown
Z1088:Col4a1	UTSW	8	11,296,859 (GRCm39)	splice site	probably benign
Z1177:Col4a1	UTSW	8	11,289,024 (GRCm39)	missense	unknown
Z1177:Col4a1	UTSW	8	11,285,218 (GRCm39)	missense	unknown

Predicted Primers

PCR Primer
(F):5'- TTGGCTACTCTGGAAAGCACACAC -3'
(R):5'- CTCTGCAAGAAGCAACTGGGTCTAC -3'

Sequencing Primer
(F):5'- TCTTCTAAGACATGGACTACCTTG -3'
(R):5'- TCTACCAGGCCCTGACG -3'

Posted On

2013-06-11