Mutagenetix > Incidental Mutation

Incidental Mutation 'R5673:Vmn2r65'

442727

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r65

Ensembl Gene

ENSMUSG00000066372

Gene Name

vomeronasal 2, receptor 65

Synonyms

ENSMUSG00000070600

MMRRC Submission

043175-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R5673 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

84589377-84613217 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to T at 84596615 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Leucine to Isoleucine at position 147 (L147I)

Ref Sequence

ENSEMBL: ENSMUSP00000036551 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044583]

AlphaFold

G3X931

Predicted Effect

probably benign
Transcript: ENSMUST00000044583
AA Change: L147I

PolyPhen 2 Score 0.232 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000036551
Gene: ENSMUSG00000066372
AA Change: L147I

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	73	435	5.6e-41	PFAM
Pfam:NCD3G	501	553	1.3e-21	PFAM
Pfam:7tm_3	584	821	2.3e-53	PFAM

Coding Region Coverage

1x: 99.3%
3x: 98.6%
10x: 97.3%
20x: 95.4%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 44 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abhd18	T	A	3: 40,877,886 (GRCm39)	M94K	probably damaging	Het
Adam2	A	G	14: 66,306,681 (GRCm39)	Y103H	probably benign	Het
Adamts17	T	C	7: 66,691,555 (GRCm39)	C580R	probably damaging	Het
Aqp5	G	A	15: 99,492,046 (GRCm39)	V98I	probably benign	Het
Brd2	A	G	17: 34,331,581 (GRCm39)		probably benign	Het
Cd177	T	C	7: 24,449,787 (GRCm39)	N566S	probably damaging	Het
Cdh23	C	T	10: 60,143,636 (GRCm39)	D2992N	probably damaging	Het
Cfap69	T	C	5: 5,646,027 (GRCm39)	T140A	possibly damaging	Het
Cfi	A	G	3: 129,648,658 (GRCm39)	I181V	probably benign	Het
Cnksr1	A	G	4: 133,962,499 (GRCm39)	L133P	probably damaging	Het
Col1a2	C	T	6: 4,539,622 (GRCm39)	L1297F	unknown	Het
Crot	T	C	5: 9,038,131 (GRCm39)	N132S	probably benign	Het
Dnah3	T	A	7: 119,550,812 (GRCm39)	Q3169L	possibly damaging	Het
Dnah8	T	A	17: 31,022,235 (GRCm39)	M3945K	probably damaging	Het
Fam186a	A	C	15: 99,839,628 (GRCm39)	H2205Q	possibly damaging	Het
Fam204a	T	C	19: 60,188,415 (GRCm39)	K216E	probably damaging	Het
Far2	A	T	6: 148,047,602 (GRCm39)	S94C	possibly damaging	Het
Gm14226	T	A	2: 154,866,842 (GRCm39)	S266R	possibly damaging	Het
Gpr137	A	G	19: 6,916,466 (GRCm39)	F276L	probably damaging	Het
Lhx3	T	C	2: 26,093,006 (GRCm39)	Y148C	probably damaging	Het
Lrfn2	T	C	17: 49,403,625 (GRCm39)	S583P	probably benign	Het
Lrrc40	A	G	3: 157,754,035 (GRCm39)		probably null	Het
Mast4	A	T	13: 102,930,580 (GRCm39)	I224N	probably damaging	Het
Meis1	T	C	11: 18,962,812 (GRCm39)	K161E	probably damaging	Het
Mptx2	G	A	1: 173,102,414 (GRCm39)	L92F	probably benign	Het
Mrgprb2	A	T	7: 48,202,121 (GRCm39)	F201L	probably benign	Het
Mroh1	T	A	15: 76,314,381 (GRCm39)	L686Q	probably damaging	Het
Mybbp1a	G	A	11: 72,335,751 (GRCm39)	V421I	probably benign	Het
Nadk	G	A	4: 155,669,642 (GRCm39)	V143I	possibly damaging	Het
Nell1	A	T	7: 49,878,594 (GRCm39)	T272S	probably damaging	Het
Npnt	A	T	3: 132,623,258 (GRCm39)	C94S	probably damaging	Het
Olfml2b	T	A	1: 170,509,698 (GRCm39)	V682E	probably damaging	Het
Pacs2	G	A	12: 113,032,618 (GRCm39)	V655M	probably damaging	Het
Pcdha1	A	C	18: 37,063,726 (GRCm39)	N130T	probably damaging	Het
Resf1	C	T	6: 149,229,491 (GRCm39)	Q846*	probably null	Het
Rnf145	G	A	11: 44,422,120 (GRCm39)	V68M	possibly damaging	Het
Sh3pxd2a	A	G	19: 47,257,105 (GRCm39)	S566P	probably damaging	Het
Sirpa	T	G	2: 129,472,022 (GRCm39)	V483G	probably damaging	Het
Sox5	T	C	6: 144,062,206 (GRCm39)	R149G	probably damaging	Het
Tbc1d4	A	G	14: 101,692,444 (GRCm39)	S1007P	probably damaging	Het
Tnfrsf8	A	G	4: 145,011,905 (GRCm39)	F317L	probably benign	Het
Ttn	T	C	2: 76,547,389 (GRCm39)	K32219R	probably damaging	Het
Vmn1r170	A	T	7: 23,305,630 (GRCm39)	T11S	possibly damaging	Het
Vmn2r77	C	T	7: 86,461,214 (GRCm39)	H847Y	probably benign	Het

Other mutations in Vmn2r65

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00942:Vmn2r65	APN	7	84,592,761 (GRCm39)	missense	probably damaging	0.99
IGL01104:Vmn2r65	APN	7	84,589,996 (GRCm39)	missense	possibly damaging	0.93
IGL01154:Vmn2r65	APN	7	84,592,729 (GRCm39)	missense	probably benign	0.00
IGL01453:Vmn2r65	APN	7	84,589,708 (GRCm39)	missense	probably damaging	1.00
IGL01675:Vmn2r65	APN	7	84,596,587 (GRCm39)	missense	probably benign	0.00
IGL01748:Vmn2r65	APN	7	84,589,507 (GRCm39)	missense	probably damaging	1.00
IGL01997:Vmn2r65	APN	7	84,589,978 (GRCm39)	missense	probably damaging	1.00
IGL02527:Vmn2r65	APN	7	84,595,724 (GRCm39)	missense	possibly damaging	0.82
IGL02617:Vmn2r65	APN	7	84,589,549 (GRCm39)	missense	probably damaging	1.00
IGL02676:Vmn2r65	APN	7	84,589,381 (GRCm39)	missense	possibly damaging	0.86
IGL03084:Vmn2r65	APN	7	84,592,354 (GRCm39)	missense	probably damaging	1.00
A4554:Vmn2r65	UTSW	7	84,595,791 (GRCm39)	missense	probably damaging	0.96
PIT4651001:Vmn2r65	UTSW	7	84,595,461 (GRCm39)	missense	probably benign	0.12
R0322:Vmn2r65	UTSW	7	84,595,756 (GRCm39)	missense	probably benign	0.01
R0453:Vmn2r65	UTSW	7	84,595,442 (GRCm39)	missense	probably benign	0.03
R0862:Vmn2r65	UTSW	7	84,592,853 (GRCm39)	missense	probably benign	0.00
R1392:Vmn2r65	UTSW	7	84,596,624 (GRCm39)	missense	probably benign	0.14
R1392:Vmn2r65	UTSW	7	84,596,624 (GRCm39)	missense	probably benign	0.14
R1508:Vmn2r65	UTSW	7	84,589,886 (GRCm39)	missense	probably benign	0.00
R1687:Vmn2r65	UTSW	7	84,590,026 (GRCm39)	missense	probably benign	0.02
R1876:Vmn2r65	UTSW	7	84,595,505 (GRCm39)	missense	probably damaging	1.00
R2136:Vmn2r65	UTSW	7	84,592,781 (GRCm39)	missense	probably damaging	0.96
R2259:Vmn2r65	UTSW	7	84,590,119 (GRCm39)	missense	possibly damaging	0.86
R2338:Vmn2r65	UTSW	7	84,590,051 (GRCm39)	missense	possibly damaging	0.86
R2880:Vmn2r65	UTSW	7	84,613,094 (GRCm39)	missense	probably damaging	1.00
R3410:Vmn2r65	UTSW	7	84,595,896 (GRCm39)	missense	probably benign	0.00
R3411:Vmn2r65	UTSW	7	84,595,896 (GRCm39)	missense	probably benign	0.00
R3770:Vmn2r65	UTSW	7	84,589,623 (GRCm39)	missense	probably damaging	1.00
R3800:Vmn2r65	UTSW	7	84,589,738 (GRCm39)	missense	possibly damaging	0.93
R3850:Vmn2r65	UTSW	7	84,595,859 (GRCm39)	missense	probably benign	0.00
R4105:Vmn2r65	UTSW	7	84,595,691 (GRCm39)	missense	probably benign	0.03
R4568:Vmn2r65	UTSW	7	84,596,677 (GRCm39)	missense	probably damaging	1.00
R4677:Vmn2r65	UTSW	7	84,613,082 (GRCm39)	missense	possibly damaging	0.55
R4768:Vmn2r65	UTSW	7	84,596,602 (GRCm39)	missense	probably damaging	1.00
R4778:Vmn2r65	UTSW	7	84,592,801 (GRCm39)	missense	possibly damaging	0.47
R5057:Vmn2r65	UTSW	7	84,589,819 (GRCm39)	missense	probably damaging	1.00
R5279:Vmn2r65	UTSW	7	84,589,849 (GRCm39)	missense	probably damaging	1.00
R5280:Vmn2r65	UTSW	7	84,595,542 (GRCm39)	missense	probably damaging	0.99
R5394:Vmn2r65	UTSW	7	84,595,862 (GRCm39)	missense	probably benign	0.04
R5487:Vmn2r65	UTSW	7	84,595,529 (GRCm39)	missense	possibly damaging	0.76
R5580:Vmn2r65	UTSW	7	84,596,802 (GRCm39)	missense	probably damaging	0.99
R5638:Vmn2r65	UTSW	7	84,590,047 (GRCm39)	missense	probably damaging	1.00
R5688:Vmn2r65	UTSW	7	84,589,900 (GRCm39)	missense	probably benign	0.00
R5935:Vmn2r65	UTSW	7	84,592,869 (GRCm39)	missense	probably benign	0.00
R6354:Vmn2r65	UTSW	7	84,589,574 (GRCm39)	missense	probably benign	0.35
R6372:Vmn2r65	UTSW	7	84,589,861 (GRCm39)	missense	probably damaging	1.00
R6924:Vmn2r65	UTSW	7	84,613,198 (GRCm39)	missense	probably benign	0.20
R7021:Vmn2r65	UTSW	7	84,596,587 (GRCm39)	missense	probably benign	0.00
R7195:Vmn2r65	UTSW	7	84,592,347 (GRCm39)	critical splice donor site	probably null
R7422:Vmn2r65	UTSW	7	84,595,569 (GRCm39)	missense	probably damaging	0.99
R7654:Vmn2r65	UTSW	7	84,590,261 (GRCm39)	missense	probably damaging	1.00
R7686:Vmn2r65	UTSW	7	84,589,744 (GRCm39)	missense	probably damaging	0.99
R7691:Vmn2r65	UTSW	7	84,592,851 (GRCm39)	missense	probably benign	0.30
R7798:Vmn2r65	UTSW	7	84,596,192 (GRCm39)	missense	probably damaging	1.00
R7798:Vmn2r65	UTSW	7	84,595,530 (GRCm39)	missense	probably benign	0.00
R8103:Vmn2r65	UTSW	7	84,595,919 (GRCm39)	missense	probably damaging	1.00
R8272:Vmn2r65	UTSW	7	84,596,817 (GRCm39)	missense	probably benign	0.02
R8303:Vmn2r65	UTSW	7	84,589,391 (GRCm39)	nonsense	probably null
R8354:Vmn2r65	UTSW	7	84,589,402 (GRCm39)	missense	possibly damaging	0.73
R8454:Vmn2r65	UTSW	7	84,589,402 (GRCm39)	missense	possibly damaging	0.73
R8489:Vmn2r65	UTSW	7	84,589,964 (GRCm39)	missense	possibly damaging	0.85
R8554:Vmn2r65	UTSW	7	84,595,960 (GRCm39)	missense	probably benign	0.00
R8680:Vmn2r65	UTSW	7	84,589,388 (GRCm39)	missense	probably benign	0.00
R8731:Vmn2r65	UTSW	7	84,589,447 (GRCm39)	nonsense	probably null
R8839:Vmn2r65	UTSW	7	84,595,489 (GRCm39)	nonsense	probably null
R8847:Vmn2r65	UTSW	7	84,590,212 (GRCm39)	missense	probably damaging	1.00
R8916:Vmn2r65	UTSW	7	84,595,665 (GRCm39)	missense	probably benign	0.21
R9254:Vmn2r65	UTSW	7	84,596,650 (GRCm39)	missense	probably damaging	0.99
R9379:Vmn2r65	UTSW	7	84,596,650 (GRCm39)	missense	probably damaging	0.99
R9392:Vmn2r65	UTSW	7	84,589,718 (GRCm39)	missense	possibly damaging	0.95
R9563:Vmn2r65	UTSW	7	84,589,880 (GRCm39)	nonsense	probably null
R9686:Vmn2r65	UTSW	7	84,590,084 (GRCm39)	missense	probably benign	0.45
X0067:Vmn2r65	UTSW	7	84,590,113 (GRCm39)	missense	probably benign	0.04
Z1088:Vmn2r65	UTSW	7	84,592,473 (GRCm39)	critical splice acceptor site	probably null
Z1177:Vmn2r65	UTSW	7	84,590,234 (GRCm39)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- GATGTCATGTATGTGCCAGAAAATG -3'
(R):5'- AACTCTAGTGATCTCTGTGTTCTG -3'

Sequencing Primer
(F):5'- ATGTGCCAGAAAATGATAATTTAAGC -3'
(R):5'- CCAGAAATATCCACCTGATTTTGGC -3'

Posted On

2016-11-09