Mutagenetix > Incidental Mutation

Incidental Mutation 'R5334:Gpr158'

423386

Institutional Source

Beutler Lab

Gene Symbol

Gpr158

Ensembl Gene

ENSMUSG00000045967

Gene Name

G protein-coupled receptor 158

Synonyms

5330427M13Rik

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5334 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

21372378-21835355 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 21832316 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Serine to Threonine at position 1139 (S1139T)

Ref Sequence

ENSEMBL: ENSMUSP00000049708 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000055946]

AlphaFold

Q8C419

Predicted Effect

probably benign
Transcript: ENSMUST00000055946
AA Change: S1139T

PolyPhen 2 Score 0.015 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000049708
Gene: ENSMUSG00000045967
AA Change: S1139T

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
low complexity region	110	125	N/A	INTRINSIC
SCOP:d1edmb_	313	359	5e-4	SMART
Blast:EGF	318	365	2e-27	BLAST
Pfam:7tm_3	426	669	1.2e-35	PFAM
low complexity region	840	863	N/A	INTRINSIC

Coding Region Coverage

1x: 99.3%
3x: 98.7%
10x: 97.3%
20x: 95.3%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 57 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Anapc15	C	T	7: 101,547,810 (GRCm39)	P68L	probably damaging	Het
Ap4s1	C	T	12: 51,785,454 (GRCm39)	S142L	probably benign	Het
Apol11a	A	G	15: 77,400,953 (GRCm39)	T147A	probably benign	Het
Aqp9	T	C	9: 71,030,292 (GRCm39)		probably null	Het
Arhgap25	T	A	6: 87,440,243 (GRCm39)	N468I	possibly damaging	Het
Arhgef2	T	A	3: 88,553,636 (GRCm39)	S924R	probably damaging	Het
Atad3a	A	T	4: 155,840,146 (GRCm39)	L144Q	probably damaging	Het
Ccbe1	T	C	18: 66,216,316 (GRCm39)	I136V	probably damaging	Het
Col24a1	C	A	3: 145,167,280 (GRCm39)	P1119Q	possibly damaging	Het
Dgkd	T	C	1: 87,865,989 (GRCm39)		probably null	Het
Dnah7a	A	G	1: 53,542,805 (GRCm39)	I2455T	probably benign	Het
Dock2	T	C	11: 34,178,643 (GRCm39)	T1795A	probably benign	Het
Dpp6	A	T	5: 27,914,538 (GRCm39)	E541V	probably benign	Het
Edem1	T	C	6: 108,825,793 (GRCm39)		probably null	Het
Fbxo41	A	G	6: 85,455,465 (GRCm39)	V573A	probably damaging	Het
Fech	A	C	18: 64,597,191 (GRCm39)	V256G	probably damaging	Het
Gad1-ps	G	A	10: 99,281,009 (GRCm39)		noncoding transcript	Het
Gal3st4	T	G	5: 138,263,983 (GRCm39)	K339Q	probably benign	Het
Gpr180	A	T	14: 118,397,468 (GRCm39)	S321C	probably damaging	Het
Grik1	CGG	CGGG	16: 87,720,082 (GRCm39)		probably null	Het
Grin2c	T	G	11: 115,146,881 (GRCm39)	N438T	possibly damaging	Het
Hcn2	T	C	10: 79,562,125 (GRCm39)	S374P	probably damaging	Het
Hmcn1	T	A	1: 150,631,123 (GRCm39)	I892F	probably damaging	Het
Ifi207	C	T	1: 173,555,097 (GRCm39)	V869I	probably benign	Het
Itgad	T	C	7: 127,788,458 (GRCm39)	Y390H	probably damaging	Het
Itgb4	C	T	11: 115,874,983 (GRCm39)	R447W	probably benign	Het
Kcnj15	A	G	16: 95,097,508 (GRCm39)	K377E	probably damaging	Het
Kcnq3	A	G	15: 65,897,073 (GRCm39)	S276P	probably damaging	Het
Klhl26	T	C	8: 70,904,968 (GRCm39)	D280G	probably damaging	Het
Lmnb2	C	T	10: 80,739,791 (GRCm39)	V376I	probably benign	Het
Lrrc37	T	C	11: 103,504,699 (GRCm39)	Q2423R	probably benign	Het
Mepce	T	A	5: 137,784,889 (GRCm39)	R29S	probably benign	Het
Mlh3	A	G	12: 85,292,535 (GRCm39)		probably null	Het
Mlip	T	A	9: 77,150,958 (GRCm39)	T33S	probably damaging	Het
Msantd5f6	T	A	4: 73,321,754 (GRCm39)	M94L	probably benign	Het
Mtpn	C	T	6: 35,489,225 (GRCm39)	D100N	probably benign	Het
Ncapg2	T	C	12: 116,390,257 (GRCm39)	I402T	probably damaging	Het
Or2aj6	C	G	16: 19,443,241 (GRCm39)	G203A	probably benign	Het
Or5m13b	T	C	2: 85,754,058 (GRCm39)	Y149H	probably damaging	Het
Pcsk5	T	C	19: 17,439,215 (GRCm39)	D1301G	probably benign	Het
Pilrb1	T	C	5: 137,853,165 (GRCm39)	M213V	probably benign	Het
Plxdc1	T	C	11: 97,846,931 (GRCm39)	T163A	possibly damaging	Het
Pnpla2	T	C	7: 141,039,406 (GRCm39)	L373P	probably damaging	Het
Prickle2	A	G	6: 92,402,665 (GRCm39)	Y52H	probably damaging	Het
Rnf220	A	G	4: 117,129,548 (GRCm39)	C294R	probably damaging	Het
Slc12a1	A	G	2: 125,059,809 (GRCm39)	D903G	probably damaging	Het
Slc34a1	T	C	13: 24,003,034 (GRCm39)	F228S	probably damaging	Het
Sptbn1	T	C	11: 30,087,364 (GRCm39)	E1025G	possibly damaging	Het
Sult1c2	T	G	17: 54,271,758 (GRCm39)	D143A	probably damaging	Het
Taar1	T	G	10: 23,796,443 (GRCm39)	I47R	probably damaging	Het
Tctn3	G	T	19: 40,591,266 (GRCm39)	Q514K	probably benign	Het
Tg	T	C	15: 66,549,904 (GRCm39)	F222S	probably damaging	Het
Tmem236	C	A	2: 14,223,871 (GRCm39)	T220K	possibly damaging	Het
Trim50	C	T	5: 135,396,330 (GRCm39)	T426M	probably damaging	Het
Vmn1r237	T	C	17: 21,534,942 (GRCm39)	F222L	probably benign	Het
Wrap73	G	A	4: 154,229,731 (GRCm39)	R34Q	probably damaging	Het
Zfp85	T	C	13: 67,899,803 (GRCm39)	Y52C	probably damaging	Het

Other mutations in Gpr158

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00340:Gpr158	APN	2	21,373,494 (GRCm39)	missense	probably damaging	1.00
IGL00469:Gpr158	APN	2	21,751,606 (GRCm39)	splice site	probably benign
IGL00706:Gpr158	APN	2	21,751,584 (GRCm39)	missense	probably damaging	1.00
IGL00780:Gpr158	APN	2	21,831,629 (GRCm39)	nonsense	probably null
IGL00885:Gpr158	APN	2	21,653,832 (GRCm39)	missense	probably damaging	1.00
IGL01339:Gpr158	APN	2	21,373,842 (GRCm39)	missense	possibly damaging	0.73
IGL01368:Gpr158	APN	2	21,831,909 (GRCm39)	missense	probably damaging	1.00
IGL02141:Gpr158	APN	2	21,788,101 (GRCm39)	missense	probably damaging	0.99
IGL02455:Gpr158	APN	2	21,373,511 (GRCm39)	missense	probably benign	0.00
IGL02554:Gpr158	APN	2	21,831,407 (GRCm39)	missense	probably benign
IGL02681:Gpr158	APN	2	21,820,441 (GRCm39)	missense	probably damaging	1.00
IGL02752:Gpr158	APN	2	21,831,638 (GRCm39)	missense	possibly damaging	0.95
IGL02756:Gpr158	APN	2	21,831,890 (GRCm39)	missense	possibly damaging	0.47
IGL03181:Gpr158	APN	2	21,787,972 (GRCm39)	missense	probably benign	0.02
IGL03258:Gpr158	APN	2	21,830,085 (GRCm39)	missense	probably damaging	1.00
IGL03386:Gpr158	APN	2	21,831,057 (GRCm39)	missense	probably damaging	1.00
PIT4810001:Gpr158	UTSW	2	21,831,682 (GRCm39)	missense	probably benign	0.01
R0071:Gpr158	UTSW	2	21,815,479 (GRCm39)	missense	probably benign	0.08
R0081:Gpr158	UTSW	2	21,831,528 (GRCm39)	missense	probably damaging	1.00
R0528:Gpr158	UTSW	2	21,830,019 (GRCm39)	missense	probably damaging	1.00
R0560:Gpr158	UTSW	2	21,830,085 (GRCm39)	missense	probably damaging	1.00
R0603:Gpr158	UTSW	2	21,820,480 (GRCm39)	missense	possibly damaging	0.67
R1560:Gpr158	UTSW	2	21,831,125 (GRCm39)	missense	probably damaging	1.00
R1561:Gpr158	UTSW	2	21,820,505 (GRCm39)	splice site	probably null
R1609:Gpr158	UTSW	2	21,788,104 (GRCm39)	missense	possibly damaging	0.61
R1741:Gpr158	UTSW	2	21,832,359 (GRCm39)	missense	probably benign	0.00
R1827:Gpr158	UTSW	2	21,832,129 (GRCm39)	missense	probably benign
R1854:Gpr158	UTSW	2	21,373,935 (GRCm39)	missense	probably damaging	1.00
R1871:Gpr158	UTSW	2	21,820,426 (GRCm39)	missense	probably damaging	1.00
R2151:Gpr158	UTSW	2	21,832,325 (GRCm39)	missense	possibly damaging	0.82
R2273:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R2275:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R3004:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R3151:Gpr158	UTSW	2	21,581,771 (GRCm39)	missense	possibly damaging	0.68
R3943:Gpr158	UTSW	2	21,373,370 (GRCm39)	missense	possibly damaging	0.65
R4238:Gpr158	UTSW	2	21,373,362 (GRCm39)	missense	probably damaging	1.00
R4379:Gpr158	UTSW	2	21,830,025 (GRCm39)	missense	probably damaging	1.00
R4381:Gpr158	UTSW	2	21,832,403 (GRCm39)	missense	probably damaging	1.00
R4464:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4467:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4496:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4506:Gpr158	UTSW	2	21,831,810 (GRCm39)	missense	probably damaging	0.99
R4530:Gpr158	UTSW	2	21,373,811 (GRCm39)	missense	probably benign	0.03
R4646:Gpr158	UTSW	2	21,831,864 (GRCm39)	missense	probably benign
R4798:Gpr158	UTSW	2	21,787,993 (GRCm39)	missense	probably damaging	1.00
R4882:Gpr158	UTSW	2	21,830,059 (GRCm39)	missense	probably damaging	0.98
R4943:Gpr158	UTSW	2	21,831,968 (GRCm39)	missense	probably damaging	1.00
R5560:Gpr158	UTSW	2	21,831,101 (GRCm39)	missense	possibly damaging	0.67
R5600:Gpr158	UTSW	2	21,832,046 (GRCm39)	missense	probably benign
R5637:Gpr158	UTSW	2	21,788,083 (GRCm39)	missense	probably benign	0.00
R5701:Gpr158	UTSW	2	21,751,520 (GRCm39)	missense	probably damaging	1.00
R5744:Gpr158	UTSW	2	21,373,331 (GRCm39)	missense	probably damaging	1.00
R5911:Gpr158	UTSW	2	21,373,932 (GRCm39)	missense	possibly damaging	0.95
R5991:Gpr158	UTSW	2	21,373,319 (GRCm39)	missense	probably damaging	0.99
R6200:Gpr158	UTSW	2	21,404,227 (GRCm39)	missense	probably damaging	0.97
R6306:Gpr158	UTSW	2	21,820,422 (GRCm39)	missense	possibly damaging	0.84
R6324:Gpr158	UTSW	2	21,815,365 (GRCm39)	missense	probably damaging	1.00
R6384:Gpr158	UTSW	2	21,831,099 (GRCm39)	missense	probably damaging	1.00
R6698:Gpr158	UTSW	2	21,831,921 (GRCm39)	missense	probably damaging	1.00
R6997:Gpr158	UTSW	2	21,653,802 (GRCm39)	missense	possibly damaging	0.46
R7086:Gpr158	UTSW	2	21,831,386 (GRCm39)	missense	probably benign	0.01
R7175:Gpr158	UTSW	2	21,373,113 (GRCm39)	missense	probably benign	0.13
R7197:Gpr158	UTSW	2	21,815,412 (GRCm39)	missense	probably damaging	0.99
R7293:Gpr158	UTSW	2	21,581,750 (GRCm39)	missense	possibly damaging	0.47
R7427:Gpr158	UTSW	2	21,832,129 (GRCm39)	missense	probably benign
R7515:Gpr158	UTSW	2	21,373,092 (GRCm39)	missense	probably damaging	1.00
R7730:Gpr158	UTSW	2	21,831,158 (GRCm39)	missense	probably damaging	1.00
R8122:Gpr158	UTSW	2	21,831,674 (GRCm39)	missense	probably benign
R8311:Gpr158	UTSW	2	21,373,701 (GRCm39)	missense	probably benign	0.00
R8754:Gpr158	UTSW	2	21,581,693 (GRCm39)	missense	probably benign	0.00
R8782:Gpr158	UTSW	2	21,404,149 (GRCm39)	missense	probably damaging	1.00
R8792:Gpr158	UTSW	2	21,558,137 (GRCm39)	missense	probably damaging	1.00
R8842:Gpr158	UTSW	2	21,581,751 (GRCm39)	missense	possibly damaging	0.88
R9009:Gpr158	UTSW	2	21,581,760 (GRCm39)	missense	probably damaging	1.00
R9102:Gpr158	UTSW	2	21,830,078 (GRCm39)	missense	probably damaging	1.00
R9150:Gpr158	UTSW	2	21,831,251 (GRCm39)	missense	probably benign	0.17
R9254:Gpr158	UTSW	2	21,373,042 (GRCm39)	start gained	probably benign
R9317:Gpr158	UTSW	2	21,832,037 (GRCm39)	missense	probably benign
R9379:Gpr158	UTSW	2	21,373,042 (GRCm39)	start gained	probably benign
R9428:Gpr158	UTSW	2	21,787,972 (GRCm39)	missense	probably benign
R9497:Gpr158	UTSW	2	21,831,825 (GRCm39)	missense	probably benign	0.00
R9667:Gpr158	UTSW	2	21,830,054 (GRCm39)	missense	probably damaging	0.99
R9681:Gpr158	UTSW	2	21,831,315 (GRCm39)	missense	probably damaging	0.99
X0062:Gpr158	UTSW	2	21,831,180 (GRCm39)	missense	probably damaging	1.00
Z1176:Gpr158	UTSW	2	21,815,501 (GRCm39)	critical splice donor site	probably null
Z1177:Gpr158	UTSW	2	21,832,083 (GRCm39)	missense	possibly damaging	0.46

Predicted Primers

PCR Primer
(F):5'- TGGGAAATCCACAGTCAGTCC -3'
(R):5'- CTATTTCTGAGGCCCTGGTATC -3'

Sequencing Primer
(F):5'- CCACAGTCAGTCCCTTTTAGAAGATG -3'
(R):5'- CTTATTTGCACTTAAAGCAGAGGAGG -3'

Posted On

2016-08-04