Incidental Mutation 'R5157:Msln'
ID 396802
Institutional Source Beutler Lab
Gene Symbol Msln
Ensembl Gene ENSMUSG00000063011
Gene Name mesothelin
Synonyms megakaryocyte potentiating factor, MPF
MMRRC Submission 042739-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.061) question?
Stock # R5157 (G1)
Quality Score 225
Status Validated
Chromosome 17
Chromosomal Location 25967587-25973352 bp(-) (GRCm39)
Type of Mutation missense
DNA Base Change (assembly) T to C at 25971957 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change Methionine to Valine at position 87 (M87V)
Ref Sequence ENSEMBL: ENSMUSP00000075279 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000047098] [ENSMUST00000075884]
AlphaFold Q61468
Predicted Effect probably benign
Transcript: ENSMUST00000047098
SMART Domains Protein: ENSMUSP00000049020
Gene: ENSMUSG00000041062

DomainStartEndE-ValueType
signal peptide 1 23 N/A INTRINSIC
Pfam:Mesothelin 29 589 2.8e-70 PFAM
low complexity region 633 653 N/A INTRINSIC
Predicted Effect probably benign
Transcript: ENSMUST00000075884
AA Change: M87V

PolyPhen 2 Score 0.009 (Sensitivity: 0.96; Specificity: 0.77)
SMART Domains Protein: ENSMUSP00000075279
Gene: ENSMUSG00000063011
AA Change: M87V

DomainStartEndE-ValueType
Pfam:Mesothelin 1 624 N/A PFAM
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.2%
  • 3x: 98.5%
  • 10x: 96.9%
  • 20x: 94.2%
Validation Efficiency 100% (57/57)
MGI Phenotype FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]
PHENOTYPE: Mice homozygous for disruptions in this allele display a normal phenotype. [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 46 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
Abca3 C T 17: 24,627,096 (GRCm39) R1266C probably damaging Het
Adap2 T C 11: 80,047,772 (GRCm39) F76S probably damaging Het
Adgb T A 10: 10,274,710 (GRCm39) H747L probably damaging Het
Aox1 T A 1: 58,109,222 (GRCm39) V670D probably damaging Het
Ap4e1 A G 2: 126,903,615 (GRCm39) D839G probably benign Het
Arhgef11 T A 3: 87,635,817 (GRCm39) probably null Het
AY074887 T C 9: 54,858,102 (GRCm39) probably benign Het
Bicd1 C G 6: 149,421,912 (GRCm39) Q878E probably benign Het
Catspere1 A T 1: 177,707,348 (GRCm39) noncoding transcript Het
Cnmd T C 14: 79,894,126 (GRCm39) Q87R probably benign Het
Col24a1 G T 3: 145,051,712 (GRCm39) G661* probably null Het
Crtap G A 9: 114,213,860 (GRCm39) L232F probably damaging Het
Ctsq T C 13: 61,184,913 (GRCm39) T258A probably benign Het
Cyp2d26 T C 15: 82,675,190 (GRCm39) Q388R probably benign Het
Ddb1 A T 19: 10,599,728 (GRCm39) T646S probably benign Het
Dnah6 G T 6: 73,172,617 (GRCm39) S280R probably benign Het
Dzank1 T C 2: 144,325,332 (GRCm39) H545R probably damaging Het
Ehhadh T A 16: 21,585,261 (GRCm39) M207L probably benign Het
Elmo2 T A 2: 165,133,627 (GRCm39) probably benign Het
Golga3 G A 5: 110,350,537 (GRCm39) A731T probably benign Het
Igsf21 T C 4: 139,755,378 (GRCm39) T426A possibly damaging Het
Kcnf1 T C 12: 17,224,742 (GRCm39) E493G probably benign Het
Lmna A T 3: 88,391,414 (GRCm39) D364E probably damaging Het
Lsr T C 7: 30,665,465 (GRCm39) Y163C probably damaging Het
Map3k20 A T 2: 72,268,558 (GRCm39) T522S probably benign Het
Mroh9 C A 1: 162,871,690 (GRCm39) A598S probably damaging Het
Or14c39 A G 7: 86,344,440 (GRCm39) K259E probably benign Het
Or1e26 T C 11: 73,480,549 (GRCm39) N5S probably damaging Het
Or4a27 T A 2: 88,559,892 (GRCm39) Q17L probably benign Het
Or5d16 T A 2: 87,773,232 (GRCm39) M247L probably benign Het
Pals1 T A 12: 78,867,589 (GRCm39) M324K possibly damaging Het
Plekhg5 T A 4: 152,192,322 (GRCm39) probably benign Het
Pprc1 G T 19: 46,053,197 (GRCm39) probably benign Het
Ptprm T A 17: 67,264,092 (GRCm39) K385I probably benign Het
Rfxap T A 3: 54,711,938 (GRCm39) N215I probably damaging Het
Slc16a7 A T 10: 125,069,333 (GRCm39) Y114* probably null Het
Smarcb1 G T 10: 75,747,628 (GRCm39) probably benign Het
Spef2 T A 15: 9,668,877 (GRCm39) R770* probably null Het
Stard9 A T 2: 120,528,342 (GRCm39) Y1533F probably benign Het
Tbcd A T 11: 121,500,853 (GRCm39) Y1142F probably benign Het
Trappc2b A T 11: 51,576,893 (GRCm39) S2T probably benign Het
Ttc23l G T 15: 10,551,636 (GRCm39) T30K possibly damaging Het
Uba7 G A 9: 107,857,246 (GRCm39) V703I probably benign Het
Upb1 T C 10: 75,248,638 (GRCm39) S53P possibly damaging Het
Zfp672 A G 11: 58,207,677 (GRCm39) S215P possibly damaging Het
Zfp978 T A 4: 147,475,437 (GRCm39) L328H probably damaging Het
Other mutations in Msln
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL01739:Msln APN 17 25,969,004 (GRCm39) critical splice donor site probably null
IGL02986:Msln APN 17 25,971,907 (GRCm39) splice site probably benign
R0349:Msln UTSW 17 25,969,250 (GRCm39) missense possibly damaging 0.69
R0562:Msln UTSW 17 25,971,980 (GRCm39) missense probably benign 0.16
R0845:Msln UTSW 17 25,969,770 (GRCm39) missense probably damaging 1.00
R1256:Msln UTSW 17 25,973,157 (GRCm39) missense probably damaging 1.00
R1305:Msln UTSW 17 25,972,001 (GRCm39) missense probably benign 0.00
R1651:Msln UTSW 17 25,972,382 (GRCm39) missense probably benign 0.00
R1930:Msln UTSW 17 25,970,896 (GRCm39) missense probably damaging 0.99
R1996:Msln UTSW 17 25,973,193 (GRCm39) start codon destroyed possibly damaging 0.94
R4532:Msln UTSW 17 25,969,698 (GRCm39) missense probably damaging 0.98
R5004:Msln UTSW 17 25,973,193 (GRCm39) start codon destroyed possibly damaging 0.94
R5159:Msln UTSW 17 25,970,563 (GRCm39) missense probably benign 0.01
R5510:Msln UTSW 17 25,968,847 (GRCm39) missense probably benign 0.15
R6385:Msln UTSW 17 25,970,115 (GRCm39) missense probably benign 0.19
R6650:Msln UTSW 17 25,969,144 (GRCm39) missense probably benign 0.00
R6682:Msln UTSW 17 25,971,993 (GRCm39) missense probably damaging 0.99
R7091:Msln UTSW 17 25,969,054 (GRCm39) missense probably damaging 1.00
R7472:Msln UTSW 17 25,969,708 (GRCm39) missense possibly damaging 0.95
R8085:Msln UTSW 17 25,971,942 (GRCm39) nonsense probably null
R8289:Msln UTSW 17 25,967,880 (GRCm39) missense possibly damaging 0.50
R9137:Msln UTSW 17 25,969,084 (GRCm39) missense probably benign 0.24
R9217:Msln UTSW 17 25,970,125 (GRCm39) missense probably benign 0.02
R9309:Msln UTSW 17 25,970,148 (GRCm39) missense possibly damaging 0.68
R9311:Msln UTSW 17 25,971,990 (GRCm39) missense probably benign 0.09
R9441:Msln UTSW 17 25,969,731 (GRCm39) missense probably benign 0.02
R9652:Msln UTSW 17 25,968,042 (GRCm39) missense probably damaging 1.00
R9723:Msln UTSW 17 25,969,008 (GRCm39) missense possibly damaging 0.55
R9798:Msln UTSW 17 25,972,771 (GRCm39) missense probably benign 0.01
X0002:Msln UTSW 17 25,971,284 (GRCm39) splice site probably null
Z1176:Msln UTSW 17 25,972,768 (GRCm39) missense possibly damaging 0.74
Predicted Primers PCR Primer
(F):5'- TTCAGTTAGGCAGCAAGAGG -3'
(R):5'- TGTCACTTGTCCCAAAGAGG -3'

Sequencing Primer
(F):5'- TTAGGCAGCAAGAGGGGCTC -3'
(R):5'- TCATGAGAAGCCCAGGGTTATG -3'
Posted On 2016-06-21