Mutagenetix > Incidental Mutation

Incidental Mutation 'R5157:Mroh9'

396758

Institutional Source

Beutler Lab

Gene Symbol

Mroh9

Ensembl Gene

ENSMUSG00000071890

Gene Name

maestro heat-like repeat family member 9

Synonyms

4921528O07Rik, Armc11

MMRRC Submission

042739-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R5157 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

162851871-162913239 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

C to A at 162871690 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Alanine to Serine at position 598 (A598S)

Ref Sequence

ENSEMBL: ENSMUSP00000094365 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000096608]

AlphaFold

G5E8L9

Predicted Effect

probably damaging
Transcript: ENSMUST00000096608
AA Change: A598S

PolyPhen 2 Score 0.959 (Sensitivity: 0.78; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000094365
Gene: ENSMUSG00000071890
AA Change: A598S

Domain	Start	End	E-Value	Type
SCOP:d1gw5b_	231	716	2e-8	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000195132

Meta Mutation Damage Score

0.1659

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.9%
20x: 94.2%

Validation Efficiency

100% (57/57)

Allele List at MGI

Other mutations in this stock

Total: 46 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abca3	C	T	17: 24,627,096 (GRCm39)	R1266C	probably damaging	Het
Adap2	T	C	11: 80,047,772 (GRCm39)	F76S	probably damaging	Het
Adgb	T	A	10: 10,274,710 (GRCm39)	H747L	probably damaging	Het
Aox1	T	A	1: 58,109,222 (GRCm39)	V670D	probably damaging	Het
Ap4e1	A	G	2: 126,903,615 (GRCm39)	D839G	probably benign	Het
Arhgef11	T	A	3: 87,635,817 (GRCm39)		probably null	Het
AY074887	T	C	9: 54,858,102 (GRCm39)		probably benign	Het
Bicd1	C	G	6: 149,421,912 (GRCm39)	Q878E	probably benign	Het
Catspere1	A	T	1: 177,707,348 (GRCm39)		noncoding transcript	Het
Cnmd	T	C	14: 79,894,126 (GRCm39)	Q87R	probably benign	Het
Col24a1	G	T	3: 145,051,712 (GRCm39)	G661*	probably null	Het
Crtap	G	A	9: 114,213,860 (GRCm39)	L232F	probably damaging	Het
Ctsq	T	C	13: 61,184,913 (GRCm39)	T258A	probably benign	Het
Cyp2d26	T	C	15: 82,675,190 (GRCm39)	Q388R	probably benign	Het
Ddb1	A	T	19: 10,599,728 (GRCm39)	T646S	probably benign	Het
Dnah6	G	T	6: 73,172,617 (GRCm39)	S280R	probably benign	Het
Dzank1	T	C	2: 144,325,332 (GRCm39)	H545R	probably damaging	Het
Ehhadh	T	A	16: 21,585,261 (GRCm39)	M207L	probably benign	Het
Elmo2	T	A	2: 165,133,627 (GRCm39)		probably benign	Het
Golga3	G	A	5: 110,350,537 (GRCm39)	A731T	probably benign	Het
Igsf21	T	C	4: 139,755,378 (GRCm39)	T426A	possibly damaging	Het
Kcnf1	T	C	12: 17,224,742 (GRCm39)	E493G	probably benign	Het
Lmna	A	T	3: 88,391,414 (GRCm39)	D364E	probably damaging	Het
Lsr	T	C	7: 30,665,465 (GRCm39)	Y163C	probably damaging	Het
Map3k20	A	T	2: 72,268,558 (GRCm39)	T522S	probably benign	Het
Msln	T	C	17: 25,971,957 (GRCm39)	M87V	probably benign	Het
Or14c39	A	G	7: 86,344,440 (GRCm39)	K259E	probably benign	Het
Or1e26	T	C	11: 73,480,549 (GRCm39)	N5S	probably damaging	Het
Or4a27	T	A	2: 88,559,892 (GRCm39)	Q17L	probably benign	Het
Or5d16	T	A	2: 87,773,232 (GRCm39)	M247L	probably benign	Het
Pals1	T	A	12: 78,867,589 (GRCm39)	M324K	possibly damaging	Het
Plekhg5	T	A	4: 152,192,322 (GRCm39)		probably benign	Het
Pprc1	G	T	19: 46,053,197 (GRCm39)		probably benign	Het
Ptprm	T	A	17: 67,264,092 (GRCm39)	K385I	probably benign	Het
Rfxap	T	A	3: 54,711,938 (GRCm39)	N215I	probably damaging	Het
Slc16a7	A	T	10: 125,069,333 (GRCm39)	Y114*	probably null	Het
Smarcb1	G	T	10: 75,747,628 (GRCm39)		probably benign	Het
Spef2	T	A	15: 9,668,877 (GRCm39)	R770*	probably null	Het
Stard9	A	T	2: 120,528,342 (GRCm39)	Y1533F	probably benign	Het
Tbcd	A	T	11: 121,500,853 (GRCm39)	Y1142F	probably benign	Het
Trappc2b	A	T	11: 51,576,893 (GRCm39)	S2T	probably benign	Het
Ttc23l	G	T	15: 10,551,636 (GRCm39)	T30K	possibly damaging	Het
Uba7	G	A	9: 107,857,246 (GRCm39)	V703I	probably benign	Het
Upb1	T	C	10: 75,248,638 (GRCm39)	S53P	possibly damaging	Het
Zfp672	A	G	11: 58,207,677 (GRCm39)	S215P	possibly damaging	Het
Zfp978	T	A	4: 147,475,437 (GRCm39)	L328H	probably damaging	Het

Other mutations in Mroh9

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00593:Mroh9	APN	1	162,873,350 (GRCm39)	missense	possibly damaging	0.89
IGL00705:Mroh9	APN	1	162,907,072 (GRCm39)	missense	probably damaging	1.00
IGL00788:Mroh9	APN	1	162,852,227 (GRCm39)	missense	probably benign	0.06
IGL00795:Mroh9	APN	1	162,888,191 (GRCm39)	missense	probably damaging	1.00
IGL00815:Mroh9	APN	1	162,866,700 (GRCm39)	missense	probably damaging	1.00
IGL01025:Mroh9	APN	1	162,875,435 (GRCm39)	missense	possibly damaging	0.67
IGL01303:Mroh9	APN	1	162,908,144 (GRCm39)	missense	probably benign	0.00
IGL01526:Mroh9	APN	1	162,883,172 (GRCm39)	missense	probably damaging	0.99
IGL01680:Mroh9	APN	1	162,875,551 (GRCm39)	splice site	probably null
IGL01823:Mroh9	APN	1	162,883,178 (GRCm39)	missense	probably benign	0.39
IGL02024:Mroh9	APN	1	162,890,071 (GRCm39)	missense	possibly damaging	0.65
IGL02213:Mroh9	APN	1	162,885,648 (GRCm39)	missense	probably damaging	1.00
IGL02455:Mroh9	APN	1	162,903,149 (GRCm39)	missense	probably benign	0.03
IGL02546:Mroh9	APN	1	162,908,145 (GRCm39)	missense	probably benign	0.04
IGL03059:Mroh9	APN	1	162,852,205 (GRCm39)	missense	possibly damaging	0.95
IGL03061:Mroh9	APN	1	162,854,071 (GRCm39)	missense	probably damaging	1.00
IGL03071:Mroh9	APN	1	162,866,766 (GRCm39)	missense	probably damaging	1.00
R0048:Mroh9	UTSW	1	162,890,056 (GRCm39)	missense	probably damaging	0.97
R0048:Mroh9	UTSW	1	162,890,056 (GRCm39)	missense	probably damaging	0.97
R0441:Mroh9	UTSW	1	162,888,331 (GRCm39)	missense	probably damaging	1.00
R0506:Mroh9	UTSW	1	162,888,205 (GRCm39)	missense	possibly damaging	0.90
R0629:Mroh9	UTSW	1	162,888,205 (GRCm39)	missense	possibly damaging	0.90
R0751:Mroh9	UTSW	1	162,893,693 (GRCm39)	missense	possibly damaging	0.84
R1301:Mroh9	UTSW	1	162,871,552 (GRCm39)	critical splice donor site	probably null
R1481:Mroh9	UTSW	1	162,854,078 (GRCm39)	missense	probably damaging	1.00
R1618:Mroh9	UTSW	1	162,852,110 (GRCm39)	missense	probably benign	0.00
R1647:Mroh9	UTSW	1	162,873,625 (GRCm39)	missense	probably damaging	1.00
R1648:Mroh9	UTSW	1	162,873,625 (GRCm39)	missense	probably damaging	1.00
R1668:Mroh9	UTSW	1	162,852,161 (GRCm39)	missense	possibly damaging	0.52
R1795:Mroh9	UTSW	1	162,884,347 (GRCm39)	missense	probably damaging	0.97
R1796:Mroh9	UTSW	1	162,873,279 (GRCm39)	missense	probably damaging	1.00
R1857:Mroh9	UTSW	1	162,866,714 (GRCm39)	missense	probably damaging	0.98
R1869:Mroh9	UTSW	1	162,854,082 (GRCm39)	missense	probably damaging	0.97
R1923:Mroh9	UTSW	1	162,903,860 (GRCm39)	missense	probably damaging	1.00
R2325:Mroh9	UTSW	1	162,854,099 (GRCm39)	splice site	probably null
R2511:Mroh9	UTSW	1	162,866,514 (GRCm39)	missense	probably benign	0.13
R2912:Mroh9	UTSW	1	162,871,572 (GRCm39)	missense	probably damaging	1.00
R2913:Mroh9	UTSW	1	162,871,572 (GRCm39)	missense	probably damaging	1.00
R2919:Mroh9	UTSW	1	162,884,341 (GRCm39)	missense	probably damaging	1.00
R2973:Mroh9	UTSW	1	162,884,338 (GRCm39)	missense	probably damaging	1.00
R3912:Mroh9	UTSW	1	162,893,638 (GRCm39)	missense	probably damaging	0.97
R4034:Mroh9	UTSW	1	162,908,122 (GRCm39)	critical splice donor site	probably null
R4551:Mroh9	UTSW	1	162,871,662 (GRCm39)	missense	probably damaging	0.98
R4656:Mroh9	UTSW	1	162,893,593 (GRCm39)	missense	probably damaging	1.00
R4662:Mroh9	UTSW	1	162,883,162 (GRCm39)	missense	probably damaging	0.97
R4743:Mroh9	UTSW	1	162,852,061 (GRCm39)	missense	probably benign	0.05
R4890:Mroh9	UTSW	1	162,854,093 (GRCm39)	missense	probably damaging	1.00
R5128:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5129:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5147:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5324:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5325:Mroh9	UTSW	1	162,888,329 (GRCm39)	missense	probably damaging	1.00
R5340:Mroh9	UTSW	1	162,908,156 (GRCm39)	start gained	probably benign
R6005:Mroh9	UTSW	1	162,903,246 (GRCm39)	missense	probably damaging	0.99
R6182:Mroh9	UTSW	1	162,893,612 (GRCm39)	nonsense	probably null
R6414:Mroh9	UTSW	1	162,902,271 (GRCm39)	missense	probably damaging	1.00
R6477:Mroh9	UTSW	1	162,903,873 (GRCm39)	missense	probably damaging	1.00
R6540:Mroh9	UTSW	1	162,866,541 (GRCm39)	missense	possibly damaging	0.87
R6541:Mroh9	UTSW	1	162,885,607 (GRCm39)	missense	possibly damaging	0.78
R6643:Mroh9	UTSW	1	162,903,130 (GRCm39)	missense	probably damaging	1.00
R6811:Mroh9	UTSW	1	162,873,610 (GRCm39)	missense	possibly damaging	0.86
R6830:Mroh9	UTSW	1	162,903,935 (GRCm39)	missense	probably benign
R7026:Mroh9	UTSW	1	162,888,251 (GRCm39)	missense	probably benign	0.00
R7052:Mroh9	UTSW	1	162,866,525 (GRCm39)	missense	possibly damaging	0.92
R7068:Mroh9	UTSW	1	162,866,750 (GRCm39)	missense	probably damaging	1.00
R7350:Mroh9	UTSW	1	162,903,858 (GRCm39)	critical splice donor site	probably null
R7545:Mroh9	UTSW	1	162,902,277 (GRCm39)	missense	possibly damaging	0.56
R7615:Mroh9	UTSW	1	162,873,601 (GRCm39)	missense	probably benign	0.40
R7743:Mroh9	UTSW	1	162,852,122 (GRCm39)	missense	probably benign
R7808:Mroh9	UTSW	1	162,866,678 (GRCm39)	missense	probably damaging	1.00
R8024:Mroh9	UTSW	1	162,866,802 (GRCm39)	missense	probably benign	0.02
R8062:Mroh9	UTSW	1	162,866,544 (GRCm39)	missense	probably damaging	1.00
R8145:Mroh9	UTSW	1	162,890,096 (GRCm39)	missense	probably benign	0.00
R8426:Mroh9	UTSW	1	162,852,294 (GRCm39)	missense	probably damaging	0.98
R8458:Mroh9	UTSW	1	162,883,250 (GRCm39)	missense	probably damaging	1.00
R8555:Mroh9	UTSW	1	162,899,595 (GRCm39)	splice site	probably null
R8960:Mroh9	UTSW	1	162,883,196 (GRCm39)	missense	probably benign	0.25
R9040:Mroh9	UTSW	1	162,890,069 (GRCm39)	missense	probably benign	0.06
R9125:Mroh9	UTSW	1	162,875,412 (GRCm39)	missense	probably benign	0.19
R9154:Mroh9	UTSW	1	162,890,030 (GRCm39)	missense
R9596:Mroh9	UTSW	1	162,893,576 (GRCm39)	missense	probably damaging	0.98
R9612:Mroh9	UTSW	1	162,866,498 (GRCm39)	missense	probably damaging	1.00
RF003:Mroh9	UTSW	1	162,885,630 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATCTGGGGCTTAACTGAACCAC -3'
(R):5'- CCAAGTCAATGGGGAAAATCC -3'

Sequencing Primer
(F):5'- TGGGGCTTAACTGAACCACAAAAAC -3'
(R):5'- ACCAATTATCAGCCCAAGT -3'

Posted On

2016-06-21