Mutagenetix > Incidental Mutation

Incidental Mutation 'R5156:Or5w15'

396711

Institutional Source

Beutler Lab

Gene Symbol

Or5w15

Ensembl Gene

ENSMUSG00000075149

Gene Name

olfactory receptor family 5 subfamily W member 15

Synonyms

MOR177-8, Olfr1138, GA_x6K02T2Q125-49242149-49241214

MMRRC Submission

042738-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.103) question?

Stock #

R5156 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

87567731-87568666 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 87568119 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Proline to Leucine at position 183 (P183L)

Ref Sequence

ENSEMBL: ENSMUSP00000148941 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000099848] [ENSMUST00000214573]

AlphaFold

Q8VFQ9

Predicted Effect

possibly damaging
Transcript: ENSMUST00000099848
AA Change: P183L

PolyPhen 2 Score 0.946 (Sensitivity: 0.80; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000097435
Gene: ENSMUSG00000075149
AA Change: P183L

Domain	Start	End	E-Value	Type
Pfam:7tm_4	31	308	2.3e-45	PFAM
Pfam:7tm_1	41	290	1.5e-18	PFAM

Predicted Effect

possibly damaging
Transcript: ENSMUST00000214573
AA Change: P183L

PolyPhen 2 Score 0.946 (Sensitivity: 0.80; Specificity: 0.95)

Meta Mutation Damage Score

0.4083

Coding Region Coverage

1x: 99.2%
3x: 98.5%
10x: 96.8%
20x: 94.0%

Validation Efficiency

100% (52/52)

MGI Phenotype

FUNCTION: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]

Allele List at MGI

Other mutations in this stock

Total: 52 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
4921513D11Rik	G	T	17: 79,935,638 (GRCm39)		probably benign	Het
Apeh	C	T	9: 107,971,486 (GRCm39)	A29T	probably damaging	Het
Arap2	A	T	5: 62,826,524 (GRCm39)	Y1013*	probably null	Het
Arhgef4	A	G	1: 34,762,355 (GRCm39)	E537G	unknown	Het
Asf1b	T	C	8: 84,682,540 (GRCm39)	F28S	probably damaging	Het
Cd46	T	A	1: 194,767,693 (GRCm39)	I123L	possibly damaging	Het
Cdca7	A	T	2: 72,309,370 (GRCm39)	T48S	probably damaging	Het
Cfap53	T	A	18: 74,492,838 (GRCm39)		probably benign	Het
Clca3a2	T	A	3: 144,511,599 (GRCm39)	T599S	probably benign	Het
Csf1	T	A	3: 107,656,252 (GRCm39)	T148S	probably benign	Het
Dmbt1	T	C	7: 130,699,400 (GRCm39)		probably null	Het
Dmpk	A	G	7: 18,818,050 (GRCm39)	D44G	probably damaging	Het
Dnajb12	T	A	10: 59,728,782 (GRCm39)	N223K	probably damaging	Het
Dync1h1	T	A	12: 110,595,264 (GRCm39)	M1392K	probably benign	Het
Edrf1	C	T	7: 133,261,908 (GRCm39)	A867V	probably damaging	Het
Efemp2	T	A	19: 5,527,706 (GRCm39)	C94S	possibly damaging	Het
Epha8	C	T	4: 136,666,037 (GRCm39)	S373N	probably benign	Het
Foxk1	A	G	5: 142,434,588 (GRCm39)	D284G	possibly damaging	Het
Fzd10	C	A	5: 128,678,366 (GRCm39)	R29S	possibly damaging	Het
Gm13991	T	C	2: 116,358,665 (GRCm39)		noncoding transcript	Het
Gm6818	A	T	7: 38,101,471 (GRCm39)		noncoding transcript	Het
Hydin	T	A	8: 111,336,333 (GRCm39)	C5037S	probably benign	Het
Ikzf1	T	A	11: 11,719,448 (GRCm39)	M492K	probably damaging	Het
Krt20	G	T	11: 99,320,879 (GRCm39)	S394R	possibly damaging	Het
Lrrc71	T	A	3: 87,653,094 (GRCm39)	R107S	probably benign	Het
Mia2	A	G	12: 59,219,323 (GRCm39)	T436A	possibly damaging	Het
Muc19	T	A	15: 91,784,614 (GRCm39)		noncoding transcript	Het
Neu4	T	C	1: 93,952,177 (GRCm39)	V182A	probably damaging	Het
Notch2	T	G	3: 98,031,626 (GRCm39)	F1167V	possibly damaging	Het
Nrap	A	G	19: 56,360,277 (GRCm39)	M189T	possibly damaging	Het
Nt5m	A	T	11: 59,765,487 (GRCm39)	I172F	probably damaging	Het
Or5b118	G	T	19: 13,449,037 (GRCm39)	K234N	probably damaging	Het
Or8k41	A	T	2: 86,313,362 (GRCm39)	C241*	probably null	Het
Plekha5	C	T	6: 140,372,254 (GRCm39)	T68M	probably damaging	Het
Ppef2	A	G	5: 92,392,461 (GRCm39)		probably null	Het
Ppp1r37	T	C	7: 19,295,900 (GRCm39)		probably benign	Het
Rfx4	T	C	10: 84,704,218 (GRCm39)	Y238H	probably damaging	Het
Sanbr	A	G	11: 23,543,424 (GRCm39)		probably null	Het
Sec13	G	A	6: 113,707,837 (GRCm39)	A161V	probably benign	Het
Serhl	G	A	15: 82,986,895 (GRCm39)		probably benign	Het
Slco4a1	T	C	2: 180,114,572 (GRCm39)	V588A	probably benign	Het
Slitrk3	T	C	3: 72,956,592 (GRCm39)	T727A	probably benign	Het
Sp100	T	A	1: 85,601,404 (GRCm39)	D241E	probably damaging	Het
Spata2	G	T	2: 167,325,494 (GRCm39)	H442N	probably damaging	Het
Speg	T	C	1: 75,404,731 (GRCm39)	V2588A	probably damaging	Het
Tnfsf12	A	G	11: 69,578,155 (GRCm39)	S141P	probably damaging	Het
Trank1	A	G	9: 111,219,762 (GRCm39)	I2166M	probably damaging	Het
Trim10	T	A	17: 37,187,948 (GRCm39)	V388E	probably damaging	Het
Ttc23l	G	T	15: 10,551,636 (GRCm39)	T30K	possibly damaging	Het
Vmn2r10	A	G	5: 109,143,466 (GRCm39)	V828A	probably benign	Het
Vmn2r75	T	A	7: 85,813,436 (GRCm39)	L455F	possibly damaging	Het
Vwa8	T	A	14: 79,221,666 (GRCm39)	S541T	probably benign	Het

Other mutations in Or5w15

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL02304:Or5w15	APN	2	87,568,330 (GRCm39)	missense	probably benign	0.01
IGL02490:Or5w15	APN	2	87,568,299 (GRCm39)	missense	probably damaging	1.00
IGL02730:Or5w15	APN	2	87,567,985 (GRCm39)	missense	probably damaging	1.00
IGL03106:Or5w15	APN	2	87,568,462 (GRCm39)	missense	probably benign	0.02
IGL03113:Or5w15	APN	2	87,568,506 (GRCm39)	missense	probably benign	0.01
R0450:Or5w15	UTSW	2	87,567,825 (GRCm39)	missense	probably damaging	0.98
R0469:Or5w15	UTSW	2	87,567,825 (GRCm39)	missense	probably damaging	0.98
R0510:Or5w15	UTSW	2	87,567,825 (GRCm39)	missense	probably damaging	0.98
R3696:Or5w15	UTSW	2	87,568,360 (GRCm39)	missense	probably benign
R3698:Or5w15	UTSW	2	87,568,360 (GRCm39)	missense	probably benign
R5149:Or5w15	UTSW	2	87,567,749 (GRCm39)	missense	probably benign
R6245:Or5w15	UTSW	2	87,568,240 (GRCm39)	missense	possibly damaging	0.54
R6701:Or5w15	UTSW	2	87,567,753 (GRCm39)	missense	probably benign
R7170:Or5w15	UTSW	2	87,568,056 (GRCm39)	missense	probably damaging	0.99
R7185:Or5w15	UTSW	2	87,568,489 (GRCm39)	missense	probably damaging	1.00
R7260:Or5w15	UTSW	2	87,568,852 (GRCm39)	splice site	probably null
R8065:Or5w15	UTSW	2	87,568,147 (GRCm39)	missense	probably damaging	1.00
R8067:Or5w15	UTSW	2	87,568,147 (GRCm39)	missense	probably damaging	1.00
R8671:Or5w15	UTSW	2	87,567,990 (GRCm39)	missense	probably damaging	0.96
R8953:Or5w15	UTSW	2	87,568,371 (GRCm39)	missense	probably benign	0.01
R9254:Or5w15	UTSW	2	87,568,372 (GRCm39)	missense	probably benign
R9356:Or5w15	UTSW	2	87,568,089 (GRCm39)	missense	probably benign	0.01
R9481:Or5w15	UTSW	2	87,568,576 (GRCm39)	missense	probably benign	0.44
R9544:Or5w15	UTSW	2	87,568,644 (GRCm39)	missense	probably benign
R9588:Or5w15	UTSW	2	87,568,644 (GRCm39)	missense	probably benign
R9646:Or5w15	UTSW	2	87,568,512 (GRCm39)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- CAGCAGTCAGGTGTGATGTACAG -3'
(R):5'- CTGGCAGTGATGGCATTTGATC -3'

Sequencing Primer
(F):5'- GGTAGAGAAAGCTTTTAACCTCCCAG -3'
(R):5'- GGCATTTGATCGATATAAGGCC -3'

Posted On

2016-06-21