Mutagenetix > Incidental Mutation

Incidental Mutation 'S24628:Adgre4'

385675

Institutional Source

Beutler Lab

Gene Symbol

Adgre4

Ensembl Gene

ENSMUSG00000032915

Gene Name

adhesion G protein-coupled receptor E4

Synonyms

EGF-TM7, Gpr127, FIRE, Emr4, D17Ertd479e

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

S24628 () of strain waterfowl

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

56056984-56160662 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

G to A at 56159288 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Isoleucine at position 658 (V658I)

Ref Sequence

ENSEMBL: ENSMUSP00000025004 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000025004]

AlphaFold

Q91ZE5

Predicted Effect

probably benign
Transcript: ENSMUST00000025004
AA Change: V658I

PolyPhen 2 Score 0.031 (Sensitivity: 0.95; Specificity: 0.82)

SMART Domains

Protein: ENSMUSP00000025004
Gene: ENSMUSG00000032915
AA Change: V658I

Domain	Start	End	E-Value	Type
signal peptide	1	36	N/A	INTRINSIC
Blast:EGF_like	38	76	2e-10	BLAST
Pfam:EGF_CA	77	117	3.6e-9	PFAM
GPS	288	338	4.03e-12	SMART
Pfam:7tm_2	343	588	5.7e-57	PFAM
low complexity region	613	628	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000176564

Coding Region Coverage

1x: 98.1%
3x: 97.0%
10x: 94.3%
20x: 88.0%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 30 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Ccdc40	T	C	11: 119,122,944 (GRCm39)	Y249H	possibly damaging	Het
D6Ertd527e	C	G	6: 87,088,506 (GRCm39)	T223S	unknown	Homo
Gbp4	G	A	5: 105,268,972 (GRCm39)	R394C	possibly damaging	Het
Gpr183	C	A	14: 122,191,888 (GRCm39)	C211F	probably damaging	Homo
Lcp1	A	T	14: 75,464,446 (GRCm39)	I556F	possibly damaging	Het
Letm1	G	A	5: 33,904,788 (GRCm39)	P513S	probably benign	Het
Letm1	G	A	5: 33,904,790 (GRCm39)	P512L	probably benign	Het
Msh3	A	G	13: 92,483,294 (GRCm39)	V283A	possibly damaging	Het
Nfkb2	G	T	19: 46,296,006 (GRCm39)	E170D	probably benign	Het
Npr3	C	A	15: 11,848,649 (GRCm39)	M439I	probably benign	Het
Or5m10	A	T	2: 85,717,782 (GRCm39)	I213F	possibly damaging	Het
Or5m9	A	T	2: 85,877,399 (GRCm39)	H191L	probably benign	Het
Pax5	G	A	4: 44,691,886 (GRCm39)	A120V	probably damaging	Het
Plcb1	A	G	2: 135,179,419 (GRCm39)	Y609C	probably damaging	Het
Plxna1	G	A	6: 89,334,318 (GRCm39)	H104Y	probably benign	Homo
Rnf213	A	T	11: 119,305,295 (GRCm39)	I509F	probably damaging	Het
Ryr2	T	C	13: 11,884,042 (GRCm39)	S213G	probably damaging	Homo
Spint1	A	G	2: 119,076,096 (GRCm39)	T231A	probably damaging	Het
Tbcel	C	A	9: 42,355,796 (GRCm39)	C139F	probably benign	Het
Thbs2	A	C	17: 14,900,235 (GRCm39)	S573A	probably benign	Het
Tmem43	C	A	6: 91,459,300 (GRCm39)	P257Q	probably benign	Homo
Tmprss13	A	G	9: 45,248,430 (GRCm39)		probably null	Het
Tnc	C	T	4: 63,936,249 (GRCm39)	G229D	probably damaging	Homo
Ugt1a10	TTCATCA	TTCA	1: 88,143,880 (GRCm39)		probably benign	Het
Vmn1r196	T	A	13: 22,478,006 (GRCm39)	V215D	probably damaging	Homo
Vmn1r22	G	T	6: 57,877,317 (GRCm39)	T220K	probably benign	Homo
Vmn2r116	G	A	17: 23,606,253 (GRCm39)	M388I	possibly damaging	Het
Zap70	A	G	1: 36,809,892 (GRCm39)	M1V	probably null	Homo
Zfp282	A	G	6: 47,874,815 (GRCm39)	D340G	probably damaging	Homo
Zfp282	T	A	6: 47,881,987 (GRCm39)	I558N	possibly damaging	Homo

Other mutations in Adgre4

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Adgre4	APN	17	56,098,915 (GRCm39)	splice site	probably benign
IGL00228:Adgre4	APN	17	56,109,135 (GRCm39)	missense	probably damaging	1.00
IGL00572:Adgre4	APN	17	56,127,648 (GRCm39)	missense	probably benign	0.00
IGL01404:Adgre4	APN	17	56,104,639 (GRCm39)	missense	possibly damaging	0.63
IGL01420:Adgre4	APN	17	56,106,785 (GRCm39)	splice site	probably benign
IGL01501:Adgre4	APN	17	56,109,002 (GRCm39)	splice site	probably benign
IGL01510:Adgre4	APN	17	56,125,760 (GRCm39)	critical splice donor site	probably null
IGL01554:Adgre4	APN	17	56,124,090 (GRCm39)	missense	probably damaging	1.00
IGL01607:Adgre4	APN	17	56,101,748 (GRCm39)	splice site	probably benign
IGL01767:Adgre4	APN	17	56,104,740 (GRCm39)	missense	probably benign	0.19
IGL02253:Adgre4	APN	17	56,067,573 (GRCm39)	missense	probably benign	0.01
IGL02358:Adgre4	APN	17	56,150,209 (GRCm39)	missense	probably benign	0.15
IGL02466:Adgre4	APN	17	56,121,188 (GRCm39)	missense	probably benign	0.42
IGL03057:Adgre4	APN	17	56,106,602 (GRCm39)	splice site	probably benign
R0070:Adgre4	UTSW	17	56,109,154 (GRCm39)	missense	probably damaging	0.98
R0070:Adgre4	UTSW	17	56,109,154 (GRCm39)	missense	probably damaging	0.98
R0111:Adgre4	UTSW	17	56,124,073 (GRCm39)	missense	possibly damaging	0.92
R0311:Adgre4	UTSW	17	56,109,010 (GRCm39)	missense	probably benign	0.36
R0366:Adgre4	UTSW	17	56,099,001 (GRCm39)	nonsense	probably null
R0415:Adgre4	UTSW	17	56,159,288 (GRCm39)	missense	probably benign	0.03
R0465:Adgre4	UTSW	17	56,092,137 (GRCm39)	splice site	probably benign
R0619:Adgre4	UTSW	17	56,127,679 (GRCm39)	missense	possibly damaging	0.52
R0685:Adgre4	UTSW	17	56,099,035 (GRCm39)	missense	probably benign	0.05
R0724:Adgre4	UTSW	17	56,159,281 (GRCm39)	missense	probably benign	0.00
R0835:Adgre4	UTSW	17	56,106,637 (GRCm39)	missense	probably damaging	1.00
R1330:Adgre4	UTSW	17	56,085,814 (GRCm39)	missense	probably benign	0.36
R1452:Adgre4	UTSW	17	56,091,996 (GRCm39)	missense	probably benign	0.35
R1960:Adgre4	UTSW	17	56,098,497 (GRCm39)	missense	probably benign
R1961:Adgre4	UTSW	17	56,098,497 (GRCm39)	missense	probably benign
R2046:Adgre4	UTSW	17	56,085,847 (GRCm39)	missense	possibly damaging	0.82
R2421:Adgre4	UTSW	17	56,085,872 (GRCm39)	missense	probably benign	0.10
R2570:Adgre4	UTSW	17	56,085,878 (GRCm39)	missense	possibly damaging	0.54
R3162:Adgre4	UTSW	17	56,109,218 (GRCm39)	splice site	probably benign
R4222:Adgre4	UTSW	17	56,092,121 (GRCm39)	missense	probably damaging	1.00
R4526:Adgre4	UTSW	17	56,092,016 (GRCm39)	nonsense	probably null
R4631:Adgre4	UTSW	17	56,121,305 (GRCm39)	missense	probably null	1.00
R4689:Adgre4	UTSW	17	56,109,096 (GRCm39)	missense	probably damaging	1.00
R4701:Adgre4	UTSW	17	56,091,971 (GRCm39)	missense	probably damaging	1.00
R4792:Adgre4	UTSW	17	56,098,491 (GRCm39)	missense	probably benign	0.00
R5205:Adgre4	UTSW	17	56,101,727 (GRCm39)	nonsense	probably null
R5210:Adgre4	UTSW	17	56,092,029 (GRCm39)	missense	probably damaging	0.97
R5358:Adgre4	UTSW	17	56,125,758 (GRCm39)	missense	probably benign	0.00
R5873:Adgre4	UTSW	17	56,159,282 (GRCm39)	missense	probably benign	0.13
R6025:Adgre4	UTSW	17	56,099,013 (GRCm39)	missense	probably benign	0.00
R6257:Adgre4	UTSW	17	56,109,133 (GRCm39)	missense	possibly damaging	0.87
R6426:Adgre4	UTSW	17	56,109,196 (GRCm39)	missense	probably benign	0.18
R6440:Adgre4	UTSW	17	56,101,744 (GRCm39)	critical splice donor site	probably null
R6484:Adgre4	UTSW	17	56,109,036 (GRCm39)	missense	possibly damaging	0.52
R6680:Adgre4	UTSW	17	56,098,959 (GRCm39)	missense	probably benign	0.09
R7086:Adgre4	UTSW	17	56,127,649 (GRCm39)	missense	probably benign	0.00
R7442:Adgre4	UTSW	17	56,159,340 (GRCm39)	missense	probably benign	0.04
R7467:Adgre4	UTSW	17	56,098,952 (GRCm39)	missense	probably benign	0.00
R7875:Adgre4	UTSW	17	56,099,016 (GRCm39)	missense	probably benign	0.00
R8007:Adgre4	UTSW	17	56,121,233 (GRCm39)	missense	probably damaging	0.99
R8096:Adgre4	UTSW	17	56,127,700 (GRCm39)	missense	probably damaging	1.00
R8172:Adgre4	UTSW	17	56,104,769 (GRCm39)	missense	probably benign	0.00
R8512:Adgre4	UTSW	17	56,125,760 (GRCm39)	critical splice donor site	probably null
R8972:Adgre4	UTSW	17	56,109,189 (GRCm39)	missense	probably damaging	1.00
R9018:Adgre4	UTSW	17	56,098,993 (GRCm39)	missense	probably benign	0.00
R9049:Adgre4	UTSW	17	56,092,094 (GRCm39)	missense	probably benign	0.05
X0010:Adgre4	UTSW	17	56,121,308 (GRCm39)	missense	probably damaging	1.00
Z1177:Adgre4	UTSW	17	56,121,152 (GRCm39)	critical splice acceptor site	probably null

Predicted Primers

PCR Primer
(F):5'- ATAGGTAGCCAAGGGATTTATTTGC -3'
(R):5'- CTAGCAATTATAGCTTCATGTGCATGG -3'

Sequencing Primer
(F):5'- TGGACTTGTTTGACCTTTATCAATC -3'
(R):5'- AAGCGTGAGTACCTGAGT -3'

Posted On

2016-05-10