Mutagenetix > Incidental Mutation

Incidental Mutation 'R4965:Morc3'

383970

Institutional Source

Beutler Lab

Gene Symbol

Morc3

Ensembl Gene

ENSMUSG00000039456

Gene Name

microrchidia 3

Synonyms

Zcwcc3, D16Jhu32e, 1110051N18Rik, 1110051N18Rik

Accession Numbers

Essential gene?

Essential (E-score: 1.000) question?

Stock #

R4965 (G1)

Quality Score

200

Status

Not validated

Chromosome

Chromosomal Location

93629009-93672961 bp(+) (GRCm39)

Type of Mutation

nonsense

DNA Base Change (assembly)

G to T at 93657475 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Glutamic Acid to Stop codon at position 25 (E25*)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044068] [ENSMUST00000201097] [ENSMUST00000201754] [ENSMUST00000202261]

AlphaFold

F7BJB9

Predicted Effect

probably null
Transcript: ENSMUST00000044068
AA Change: E400*

SMART Domains

Protein: ENSMUSP00000040152
Gene: ENSMUSG00000039456
AA Change: E400*

Domain	Start	End	E-Value	Type
Pfam:HATPase_c	25	130	8e-8	PFAM
Pfam:HATPase_c_3	26	164	1.1e-18	PFAM
Pfam:zf-CW	409	452	2.7e-19	PFAM
low complexity region	461	472	N/A	INTRINSIC
low complexity region	630	639	N/A	INTRINSIC
coiled coil region	765	871	N/A	INTRINSIC

Predicted Effect

probably null
Transcript: ENSMUST00000201097
AA Change: E329*

SMART Domains

Protein: ENSMUSP00000144058
Gene: ENSMUSG00000039456
AA Change: E329*

Domain	Start	End	E-Value	Type
Blast:HATPase_c	1	70	4e-39	BLAST
Pfam:zf-CW	340	383	1.2e-16	PFAM
low complexity region	392	403	N/A	INTRINSIC
low complexity region	557	566	N/A	INTRINSIC
coiled coil region	692	798	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000201706

Predicted Effect

probably benign
Transcript: ENSMUST00000201754

SMART Domains

Protein: ENSMUSP00000144357
Gene: ENSMUSG00000039456

Domain	Start	End	E-Value	Type
Pfam:HATPase_c	25	91	1.3e-6	PFAM
Pfam:HATPase_c_3	26	92	1.2e-8	PFAM

Predicted Effect

probably null
Transcript: ENSMUST00000202261
AA Change: E400*

SMART Domains

Protein: ENSMUSP00000144369
Gene: ENSMUSG00000039456
AA Change: E400*

Domain	Start	End	E-Value	Type
Pfam:HATPase_c	25	130	8e-8	PFAM
Pfam:HATPase_c_3	26	164	1.1e-18	PFAM
Pfam:zf-CW	409	452	2.7e-19	PFAM
low complexity region	461	472	N/A	INTRINSIC
low complexity region	630	639	N/A	INTRINSIC
coiled coil region	765	871	N/A	INTRINSIC

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000232425

Predicted Effect

probably null
Transcript: ENSMUST00000232639
AA Change: E25*

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 95.7%
20x: 90.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene encodes a protein that localizes to the nuclear matrix and forms nuclear bodies via an ATP-dependent mechanism. The protein is predicted to have coiled-coil and zinc finger domains and has RNA binding activity. Alternative splicing produces multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Feb 2016]
PHENOTYPE: Mice homozygous for a null allele die at or within a day of birth. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 117 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 137,775,433 (GRCm39)	T1541A	probably benign	Het
9530002B09Rik	T	C	4: 122,594,285 (GRCm39)	M59T	probably benign	Het
Adam18	C	T	8: 25,131,827 (GRCm39)	C428Y	probably damaging	Het
Adcy5	A	G	16: 35,098,872 (GRCm39)	E700G	possibly damaging	Het
Adprh	G	T	16: 38,266,142 (GRCm39)	Y333*	probably null	Het
Agfg2	C	A	5: 137,665,439 (GRCm39)		probably null	Het
Akip1	A	T	7: 109,310,961 (GRCm39)	E167V	probably damaging	Het
Akr1c21	A	T	13: 4,630,304 (GRCm39)	Q199L	probably damaging	Het
Aldh9a1	C	A	1: 167,193,358 (GRCm39)	A455E	probably damaging	Het
Amph	G	A	13: 19,321,869 (GRCm39)	S520N	probably benign	Het
Ankrd52	A	G	10: 128,226,376 (GRCm39)	D1006G	probably benign	Het
Ap5z1	A	C	5: 142,453,431 (GRCm39)	Q133P	probably damaging	Het
Babam1	C	T	8: 71,857,032 (GRCm39)	A331V	possibly damaging	Het
Btc	T	C	5: 91,510,160 (GRCm39)		probably null	Het
Cacna2d3	A	G	14: 28,704,289 (GRCm39)	F831L	probably benign	Het
Cadm3	G	A	1: 173,164,664 (GRCm39)	P372L	probably damaging	Het
Capn5	A	T	7: 97,775,624 (GRCm39)	M439K	probably damaging	Het
Carf	T	C	1: 60,189,796 (GRCm39)	S639P	probably damaging	Het
Casp12	C	T	9: 5,352,250 (GRCm39)	R81C	probably benign	Het
Ces2e	G	T	8: 105,660,330 (GRCm39)	R555M	probably benign	Het
Cfap54	T	A	10: 92,902,661 (GRCm39)	I164F	probably benign	Het
Cltc	C	T	11: 86,598,327 (GRCm39)	V1012I	probably damaging	Het
Cmya5	G	A	13: 93,232,295 (GRCm39)	T931I	possibly damaging	Het
Cntn6	A	G	6: 104,751,435 (GRCm39)	I364V	probably damaging	Het
Cntnap1	A	T	11: 101,068,251 (GRCm39)	I59F	possibly damaging	Het
Cop1	T	C	1: 159,067,167 (GRCm39)	M80T	probably damaging	Het
Cplx2	A	G	13: 54,527,460 (GRCm39)	S115G	possibly damaging	Het
Crtac1	A	G	19: 42,307,179 (GRCm39)	Y195H	probably damaging	Het
Csn1s2a	A	C	5: 87,929,697 (GRCm39)	S99R	possibly damaging	Het
Csn1s2b	T	A	5: 87,961,820 (GRCm39)	D41E	possibly damaging	Het
Cul9	C	T	17: 46,849,451 (GRCm39)	D565N	probably damaging	Het
Cux1	A	T	5: 136,340,410 (GRCm39)	N625K	possibly damaging	Het
Cyp2c37	A	T	19: 40,000,206 (GRCm39)	M443L	possibly damaging	Het
Cyp2s1	G	A	7: 25,508,710 (GRCm39)	T244I	possibly damaging	Het
Dgkh	C	T	14: 78,861,861 (GRCm39)	V135M	probably damaging	Het
Dtl	T	C	1: 191,278,677 (GRCm39)	E395G	possibly damaging	Het
Dyrk1a	G	T	16: 94,492,854 (GRCm39)	G658*	probably null	Het
Erlin2	T	C	8: 27,519,623 (GRCm39)	F117S	probably damaging	Het
Fkbp8	A	G	8: 70,984,173 (GRCm39)		probably null	Het
Fras1	T	C	5: 96,874,439 (GRCm39)	F2288S	possibly damaging	Het
Frmd3	A	G	4: 74,071,837 (GRCm39)	T240A	probably damaging	Het
H2-D1	A	G	17: 35,482,881 (GRCm39)	Y137C	probably damaging	Het
Helz2	A	G	2: 180,882,709 (GRCm39)	V28A	possibly damaging	Het
Hydin	A	G	8: 111,124,727 (GRCm39)	I579V	probably benign	Het
Il6	A	T	5: 30,218,491 (GRCm39)	Y29F	possibly damaging	Het
Ildr2	A	G	1: 166,135,409 (GRCm39)	D368G	probably damaging	Het
Junb	T	A	8: 85,704,788 (GRCm39)	I91F	probably damaging	Het
Kat2a	C	A	11: 100,603,029 (GRCm39)		probably benign	Het
Kat2a	A	T	11: 100,603,030 (GRCm39)		probably benign	Het
Kcnh5	T	A	12: 75,011,925 (GRCm39)	T665S	probably benign	Het
Kdm1b	A	T	13: 47,227,843 (GRCm39)	D608V	probably damaging	Het
Krcc1	A	G	6: 71,261,621 (GRCm39)	K218E	probably damaging	Het
Krt8	C	T	15: 101,905,386 (GRCm39)	V488M	probably benign	Het
Lzts1	C	T	8: 69,591,414 (GRCm39)	A245T	probably benign	Het
Mcm6	T	A	1: 128,287,223 (GRCm39)	Q27L	probably damaging	Het
Mfsd4b3-ps	G	T	10: 39,823,686 (GRCm39)	Y191*	probably null	Het
Mgme1	C	T	2: 144,118,324 (GRCm39)	Q199*	probably null	Het
Mgme1	T	C	2: 144,121,540 (GRCm39)	L332P	probably benign	Het
Mroh7	G	A	4: 106,548,184 (GRCm39)	A1098V	possibly damaging	Het
Mtrf1	G	A	14: 79,644,027 (GRCm39)	R174H	probably benign	Het
Mybpc3	G	A	2: 90,949,592 (GRCm39)	G45D	possibly damaging	Het
Mycbpap	A	T	11: 94,395,764 (GRCm39)	N733K	probably damaging	Het
N4bp1	T	C	8: 87,578,314 (GRCm39)	I684V	possibly damaging	Het
Nav2	A	T	7: 49,202,625 (GRCm39)	R1470*	probably null	Het
Ndufa9	A	T	6: 126,799,026 (GRCm39)	S364T	probably benign	Het
Nipal4	C	A	11: 46,052,837 (GRCm39)	A43S	possibly damaging	Het
Nlrp1a	T	A	11: 70,983,141 (GRCm39)	Y1275F	possibly damaging	Het
Nova1	A	T	12: 46,767,618 (GRCm39)	L8*	probably null	Het
Odam	G	T	5: 88,037,967 (GRCm39)	G181*	probably null	Het
Optn	T	A	2: 5,026,190 (GRCm39)	Q576L	probably benign	Het
Or2c1	T	C	16: 3,657,434 (GRCm39)	L199P	probably damaging	Het
Or4k47	T	C	2: 111,451,879 (GRCm39)	D180G	probably damaging	Het
Or4l1	C	T	14: 50,166,354 (GRCm39)	V216I	probably benign	Het
Or51a39	A	T	7: 102,362,702 (GRCm39)	I306N	probably damaging	Het
Or5as1	T	A	2: 86,981,003 (GRCm39)	M1L	possibly damaging	Het
Or5h18	A	T	16: 58,847,696 (GRCm39)	D191E	probably damaging	Het
Or8s2	T	C	15: 98,277,030 (GRCm39)		probably benign	Het
Patl2	G	T	2: 121,959,329 (GRCm39)	S45*	probably null	Het
Pde2a	G	A	7: 101,152,140 (GRCm39)	G349E	probably benign	Het
Pdlim2	T	A	14: 70,405,464 (GRCm39)		probably benign	Het
Per1	T	C	11: 68,995,227 (GRCm39)	V653A	probably benign	Het
Phlda2	A	G	7: 143,056,005 (GRCm39)	S75P	probably damaging	Het
Poldip3	A	T	15: 83,021,706 (GRCm39)	M167K	possibly damaging	Het
Potegl	C	A	2: 23,135,127 (GRCm39)	T312K	probably benign	Het
Prpf38a	T	C	4: 108,436,278 (GRCm39)	I12V	probably benign	Het
Prrc1	G	A	18: 57,507,622 (GRCm39)	V259I	possibly damaging	Het
Ptges3l	A	T	11: 101,315,448 (GRCm39)	M1K	probably null	Het
Rdh5	A	G	10: 128,749,653 (GRCm39)	Y296H	probably damaging	Het
Resf1	G	T	6: 149,229,896 (GRCm39)	G981*	probably null	Het
Rnf2	T	A	1: 151,348,968 (GRCm39)	K51*	probably null	Het
Rpusd3	C	A	6: 113,393,809 (GRCm39)	R215L	probably benign	Het
Rsph4a	A	T	10: 33,785,236 (GRCm39)	E382D	probably damaging	Het
S1pr2	G	A	9: 20,879,745 (GRCm39)	Q28*	probably null	Het
Sesn1	A	T	10: 41,771,005 (GRCm39)	I179F	probably damaging	Het
Setd3	T	A	12: 108,079,630 (GRCm39)	E291V	probably benign	Het
Shc1	G	T	3: 89,334,303 (GRCm39)	R323L	probably damaging	Het
Slc22a5	T	C	11: 53,782,352 (GRCm39)	D5G	possibly damaging	Het
Slc6a19	T	C	13: 73,848,677 (GRCm39)	K26E	probably benign	Het
Slc9a3	A	G	13: 74,312,412 (GRCm39)	N670D	possibly damaging	Het
Spata19	A	T	9: 27,311,761 (GRCm39)	I127L	probably benign	Het
Speg	G	T	1: 75,404,347 (GRCm39)	V2751L	probably damaging	Het
Sptbn2	A	G	19: 4,779,337 (GRCm39)	D298G	probably benign	Het
Srm	T	C	4: 148,678,640 (GRCm39)	V289A	possibly damaging	Het
Stip1	C	T	19: 7,012,938 (GRCm39)	A49T	probably benign	Het
Tas2r118	C	A	6: 23,969,627 (GRCm39)	V145F	probably benign	Het
Tbc1d12	A	T	19: 38,854,169 (GRCm39)	K284*	probably null	Het
Tfcp2	T	C	15: 100,423,531 (GRCm39)	H125R	probably damaging	Het
Tfdp1	T	C	8: 13,423,073 (GRCm39)	V206A	probably damaging	Het
Tgtp2	A	G	11: 48,950,237 (GRCm39)	W112R	probably damaging	Het
Tmem71	T	G	15: 66,410,710 (GRCm39)	M221L	probably benign	Het
Tpcn1	T	C	5: 120,685,552 (GRCm39)	N436S	possibly damaging	Het
Usp4	C	T	9: 108,239,819 (GRCm39)	L183F	probably damaging	Het
Vmn2r55	A	T	7: 12,404,478 (GRCm39)	N308K	possibly damaging	Het
Zfp106	T	A	2: 120,364,400 (GRCm39)	D669V	probably damaging	Het
Zfp108	A	T	7: 23,959,573 (GRCm39)	I55L	probably benign	Het
Zfp512b	A	T	2: 181,228,131 (GRCm39)	S8R	probably damaging	Het
Zfp827	T	C	8: 79,787,910 (GRCm39)	S359P	probably benign	Het

Other mutations in Morc3

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00561:Morc3	APN	16	93,670,283 (GRCm39)	critical splice donor site	probably null
IGL01015:Morc3	APN	16	93,659,534 (GRCm39)	missense	probably damaging	1.00
IGL01374:Morc3	APN	16	93,641,101 (GRCm39)	missense	probably damaging	1.00
IGL01634:Morc3	APN	16	93,670,125 (GRCm39)	missense	probably benign	0.02
IGL01845:Morc3	APN	16	93,657,455 (GRCm39)	missense	probably damaging	0.96
IGL02202:Morc3	APN	16	93,667,749 (GRCm39)	missense	probably benign	0.01
IGL02478:Morc3	APN	16	93,661,844 (GRCm39)	splice site	probably benign
IGL03026:Morc3	APN	16	93,659,612 (GRCm39)	splice site	probably benign
IGL03115:Morc3	APN	16	93,667,971 (GRCm39)	missense	probably damaging	0.99
Ballista	UTSW	16	93,638,271 (GRCm39)	missense	probably damaging	1.00
mindy	UTSW	16	93,663,421 (GRCm39)	missense	probably benign	0.03
Pfaff	UTSW	16	93,659,572 (GRCm39)	missense	probably damaging	0.96
shield	UTSW	16	93,671,700 (GRCm39)	missense	probably damaging	0.98
sparkle	UTSW	16	93,667,362 (GRCm39)	missense	probably damaging	1.00
Stooges	UTSW	16	93,638,275 (GRCm39)	missense	probably damaging	1.00
Sword	UTSW	16	93,671,645 (GRCm39)	critical splice acceptor site	probably null
R0173:Morc3	UTSW	16	93,629,094 (GRCm39)	splice site	probably null
R0413:Morc3	UTSW	16	93,667,362 (GRCm39)	missense	probably damaging	1.00
R0639:Morc3	UTSW	16	93,650,738 (GRCm39)	missense	probably damaging	1.00
R0842:Morc3	UTSW	16	93,670,284 (GRCm39)	critical splice donor site	probably null
R1134:Morc3	UTSW	16	93,667,557 (GRCm39)	missense	probably benign
R1162:Morc3	UTSW	16	93,649,996 (GRCm39)	missense	probably damaging	1.00
R1498:Morc3	UTSW	16	93,650,743 (GRCm39)	missense	probably damaging	1.00
R1520:Morc3	UTSW	16	93,641,129 (GRCm39)	missense	probably damaging	0.96
R1603:Morc3	UTSW	16	93,663,391 (GRCm39)	missense	probably benign
R1622:Morc3	UTSW	16	93,671,694 (GRCm39)	missense	probably benign	0.28
R1630:Morc3	UTSW	16	93,663,421 (GRCm39)	missense	probably benign	0.03
R1818:Morc3	UTSW	16	93,652,398 (GRCm39)	missense	probably damaging	1.00
R1902:Morc3	UTSW	16	93,667,385 (GRCm39)	missense	probably damaging	1.00
R2090:Morc3	UTSW	16	93,663,341 (GRCm39)	missense	probably benign	0.23
R2261:Morc3	UTSW	16	93,650,109 (GRCm39)	splice site	probably benign
R2360:Morc3	UTSW	16	93,638,275 (GRCm39)	missense	probably damaging	1.00
R2407:Morc3	UTSW	16	93,641,215 (GRCm39)	critical splice donor site	probably null
R2519:Morc3	UTSW	16	93,659,427 (GRCm39)	splice site	probably null
R3736:Morc3	UTSW	16	93,671,700 (GRCm39)	missense	probably damaging	0.98
R3873:Morc3	UTSW	16	93,659,324 (GRCm39)	missense	probably damaging	0.99
R4114:Morc3	UTSW	16	93,670,227 (GRCm39)	missense	probably benign	0.01
R4115:Morc3	UTSW	16	93,670,227 (GRCm39)	missense	probably benign	0.01
R4116:Morc3	UTSW	16	93,670,227 (GRCm39)	missense	probably benign	0.01
R4472:Morc3	UTSW	16	93,671,645 (GRCm39)	critical splice acceptor site	probably null
R4609:Morc3	UTSW	16	93,661,856 (GRCm39)	missense	probably benign	0.01
R4708:Morc3	UTSW	16	93,670,126 (GRCm39)	missense	probably benign	0.19
R4883:Morc3	UTSW	16	93,667,250 (GRCm39)	critical splice acceptor site	probably null
R4945:Morc3	UTSW	16	93,668,082 (GRCm39)	missense	probably damaging	1.00
R5399:Morc3	UTSW	16	93,659,427 (GRCm39)	splice site	probably null
R5481:Morc3	UTSW	16	93,659,543 (GRCm39)	missense	probably damaging	0.99
R5540:Morc3	UTSW	16	93,644,268 (GRCm39)	missense	probably benign
R5970:Morc3	UTSW	16	93,663,341 (GRCm39)	missense	possibly damaging	0.65
R6006:Morc3	UTSW	16	93,663,381 (GRCm39)	missense	possibly damaging	0.67
R6044:Morc3	UTSW	16	93,663,330 (GRCm39)	missense	probably benign	0.02
R6045:Morc3	UTSW	16	93,671,733 (GRCm39)	missense	probably damaging	1.00
R6155:Morc3	UTSW	16	93,659,313 (GRCm39)	missense	possibly damaging	0.94
R6165:Morc3	UTSW	16	93,638,271 (GRCm39)	missense	probably damaging	1.00
R6225:Morc3	UTSW	16	93,642,082 (GRCm39)	nonsense	probably null
R6240:Morc3	UTSW	16	93,659,572 (GRCm39)	missense	probably damaging	0.96
R6835:Morc3	UTSW	16	93,644,309 (GRCm39)	missense	probably damaging	1.00
R6918:Morc3	UTSW	16	93,650,023 (GRCm39)	missense	probably benign	0.36
R6944:Morc3	UTSW	16	93,667,460 (GRCm39)	missense	probably benign
R7311:Morc3	UTSW	16	93,646,061 (GRCm39)	missense	probably damaging	1.00
R7398:Morc3	UTSW	16	93,671,748 (GRCm39)	missense	probably damaging	1.00
R7553:Morc3	UTSW	16	93,667,824 (GRCm39)	missense	probably damaging	0.98
R8056:Morc3	UTSW	16	93,642,064 (GRCm39)	missense	probably benign	0.07
R8299:Morc3	UTSW	16	93,650,088 (GRCm39)	missense	probably damaging	1.00
R8317:Morc3	UTSW	16	93,659,417 (GRCm39)	missense	probably benign	0.25
R8542:Morc3	UTSW	16	93,644,319 (GRCm39)	critical splice donor site	probably null
R8697:Morc3	UTSW	16	93,667,908 (GRCm39)	missense	probably benign	0.00
R8739:Morc3	UTSW	16	93,657,398 (GRCm39)	missense	probably damaging	1.00
R9072:Morc3	UTSW	16	93,667,482 (GRCm39)	missense	probably benign	0.00
R9235:Morc3	UTSW	16	93,659,321 (GRCm39)	missense	probably damaging	1.00
R9305:Morc3	UTSW	16	93,667,302 (GRCm39)	missense	probably benign	0.00
R9405:Morc3	UTSW	16	93,642,036 (GRCm39)	missense	probably damaging	1.00
R9431:Morc3	UTSW	16	93,667,771 (GRCm39)	nonsense	probably null
R9440:Morc3	UTSW	16	93,649,975 (GRCm39)	critical splice acceptor site	probably null
R9524:Morc3	UTSW	16	93,667,401 (GRCm39)	missense	probably benign	0.09
R9571:Morc3	UTSW	16	93,641,107 (GRCm39)	missense	possibly damaging	0.89
X0023:Morc3	UTSW	16	93,644,287 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- TAGGAGCTTACAGTGGCCTTTATAG -3'
(R):5'- TAGTTTCTGCAAGCAACAGGC -3'

Sequencing Primer
(F):5'- AGCATGCTTGAATGGAGATTTTTCC -3'
(R):5'- CACAGGTAGTGCAGAGTGGC -3'

Posted On

2016-04-27