Mutagenetix > Incidental Mutation

Incidental Mutation 'R4965:Helz2'

383875

Institutional Source

Beutler Lab

Gene Symbol

Helz2

Ensembl Gene

ENSMUSG00000027580

Gene Name

helicase with zinc finger 2, transcriptional coactivator

Synonyms

BC006779

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4965 (G1)

Quality Score

120

Status

Not validated

Chromosome

Chromosomal Location

180869408-180883820 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 180882709 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Valine to Alanine at position 28 (V28A)

Ref Sequence

ENSEMBL: ENSMUSP00000112917 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000094203] [ENSMUST00000108831] [ENSMUST00000121484]

AlphaFold

no structure available at present

Predicted Effect

possibly damaging
Transcript: ENSMUST00000094203
AA Change: V28A

PolyPhen 2 Score 0.745 (Sensitivity: 0.85; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000091756
Gene: ENSMUSG00000027580
AA Change: V28A

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000108831
AA Change: V28A

PolyPhen 2 Score 0.223 (Sensitivity: 0.91; Specificity: 0.88)

SMART Domains

Protein: ENSMUSP00000104459
Gene: ENSMUSG00000027580
AA Change: V28A

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
AAA	782	973	1.41e-2	SMART
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
AAA	2462	2713	1.48e0	SMART
SCOP:d1pjr_2	2793	2838	2e-6	SMART

Predicted Effect

possibly damaging
Transcript: ENSMUST00000121484
AA Change: V28A

PolyPhen 2 Score 0.786 (Sensitivity: 0.85; Specificity: 0.93)

SMART Domains

Protein: ENSMUSP00000112917
Gene: ENSMUSG00000027580
AA Change: V28A

Domain	Start	End	E-Value	Type
low complexity region	509	517	N/A	INTRINSIC
Pfam:AAA_11	761	877	3.9e-10	PFAM
Pfam:AAA_19	780	849	1.7e-7	PFAM
Pfam:AAA_11	870	952	2e-15	PFAM
Pfam:AAA_12	958	1162	3.8e-26	PFAM
low complexity region	1238	1263	N/A	INTRINSIC
low complexity region	1284	1291	N/A	INTRINSIC
RNB	1567	1924	2.45e-87	SMART
low complexity region	2056	2067	N/A	INTRINSIC
low complexity region	2242	2259	N/A	INTRINSIC
Pfam:AAA_11	2400	2653	4e-42	PFAM
Pfam:AAA_12	2660	2866	2e-47	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000155049

Coding Region Coverage

1x: 99.0%
3x: 98.2%
10x: 95.7%
20x: 90.1%

Validation Efficiency

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] The protein encoded by this gene is a nuclear transcriptional co-activator for peroxisome proliferator activated receptor alpha. The encoded protein contains a zinc finger and is a helicase that appears to be part of the peroxisome proliferator activated receptor alpha interacting complex. This gene is a member of the DNA2/NAM7 helicase gene family. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
PHENOTYPE: Mice homozygous for a knock-out allele exhibit slower weight gain, hyperleptinemia, increased oxygen consumption, decreased respiratory quotient, decreased liver triglyceride level and ameliorated hyperlipidemia and hepatosteatosis when fed a high-fat diet. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 117 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	A	G	3: 137,775,433 (GRCm39)	T1541A	probably benign	Het
9530002B09Rik	T	C	4: 122,594,285 (GRCm39)	M59T	probably benign	Het
Adam18	C	T	8: 25,131,827 (GRCm39)	C428Y	probably damaging	Het
Adcy5	A	G	16: 35,098,872 (GRCm39)	E700G	possibly damaging	Het
Adprh	G	T	16: 38,266,142 (GRCm39)	Y333*	probably null	Het
Agfg2	C	A	5: 137,665,439 (GRCm39)		probably null	Het
Akip1	A	T	7: 109,310,961 (GRCm39)	E167V	probably damaging	Het
Akr1c21	A	T	13: 4,630,304 (GRCm39)	Q199L	probably damaging	Het
Aldh9a1	C	A	1: 167,193,358 (GRCm39)	A455E	probably damaging	Het
Amph	G	A	13: 19,321,869 (GRCm39)	S520N	probably benign	Het
Ankrd52	A	G	10: 128,226,376 (GRCm39)	D1006G	probably benign	Het
Ap5z1	A	C	5: 142,453,431 (GRCm39)	Q133P	probably damaging	Het
Babam1	C	T	8: 71,857,032 (GRCm39)	A331V	possibly damaging	Het
Btc	T	C	5: 91,510,160 (GRCm39)		probably null	Het
Cacna2d3	A	G	14: 28,704,289 (GRCm39)	F831L	probably benign	Het
Cadm3	G	A	1: 173,164,664 (GRCm39)	P372L	probably damaging	Het
Capn5	A	T	7: 97,775,624 (GRCm39)	M439K	probably damaging	Het
Carf	T	C	1: 60,189,796 (GRCm39)	S639P	probably damaging	Het
Casp12	C	T	9: 5,352,250 (GRCm39)	R81C	probably benign	Het
Ces2e	G	T	8: 105,660,330 (GRCm39)	R555M	probably benign	Het
Cfap54	T	A	10: 92,902,661 (GRCm39)	I164F	probably benign	Het
Cltc	C	T	11: 86,598,327 (GRCm39)	V1012I	probably damaging	Het
Cmya5	G	A	13: 93,232,295 (GRCm39)	T931I	possibly damaging	Het
Cntn6	A	G	6: 104,751,435 (GRCm39)	I364V	probably damaging	Het
Cntnap1	A	T	11: 101,068,251 (GRCm39)	I59F	possibly damaging	Het
Cop1	T	C	1: 159,067,167 (GRCm39)	M80T	probably damaging	Het
Cplx2	A	G	13: 54,527,460 (GRCm39)	S115G	possibly damaging	Het
Crtac1	A	G	19: 42,307,179 (GRCm39)	Y195H	probably damaging	Het
Csn1s2a	A	C	5: 87,929,697 (GRCm39)	S99R	possibly damaging	Het
Csn1s2b	T	A	5: 87,961,820 (GRCm39)	D41E	possibly damaging	Het
Cul9	C	T	17: 46,849,451 (GRCm39)	D565N	probably damaging	Het
Cux1	A	T	5: 136,340,410 (GRCm39)	N625K	possibly damaging	Het
Cyp2c37	A	T	19: 40,000,206 (GRCm39)	M443L	possibly damaging	Het
Cyp2s1	G	A	7: 25,508,710 (GRCm39)	T244I	possibly damaging	Het
Dgkh	C	T	14: 78,861,861 (GRCm39)	V135M	probably damaging	Het
Dtl	T	C	1: 191,278,677 (GRCm39)	E395G	possibly damaging	Het
Dyrk1a	G	T	16: 94,492,854 (GRCm39)	G658*	probably null	Het
Erlin2	T	C	8: 27,519,623 (GRCm39)	F117S	probably damaging	Het
Fkbp8	A	G	8: 70,984,173 (GRCm39)		probably null	Het
Fras1	T	C	5: 96,874,439 (GRCm39)	F2288S	possibly damaging	Het
Frmd3	A	G	4: 74,071,837 (GRCm39)	T240A	probably damaging	Het
H2-D1	A	G	17: 35,482,881 (GRCm39)	Y137C	probably damaging	Het
Hydin	A	G	8: 111,124,727 (GRCm39)	I579V	probably benign	Het
Il6	A	T	5: 30,218,491 (GRCm39)	Y29F	possibly damaging	Het
Ildr2	A	G	1: 166,135,409 (GRCm39)	D368G	probably damaging	Het
Junb	T	A	8: 85,704,788 (GRCm39)	I91F	probably damaging	Het
Kat2a	C	A	11: 100,603,029 (GRCm39)		probably benign	Het
Kat2a	A	T	11: 100,603,030 (GRCm39)		probably benign	Het
Kcnh5	T	A	12: 75,011,925 (GRCm39)	T665S	probably benign	Het
Kdm1b	A	T	13: 47,227,843 (GRCm39)	D608V	probably damaging	Het
Krcc1	A	G	6: 71,261,621 (GRCm39)	K218E	probably damaging	Het
Krt8	C	T	15: 101,905,386 (GRCm39)	V488M	probably benign	Het
Lzts1	C	T	8: 69,591,414 (GRCm39)	A245T	probably benign	Het
Mcm6	T	A	1: 128,287,223 (GRCm39)	Q27L	probably damaging	Het
Mfsd4b3-ps	G	T	10: 39,823,686 (GRCm39)	Y191*	probably null	Het
Mgme1	C	T	2: 144,118,324 (GRCm39)	Q199*	probably null	Het
Mgme1	T	C	2: 144,121,540 (GRCm39)	L332P	probably benign	Het
Morc3	G	T	16: 93,657,475 (GRCm39)	E25*	probably null	Het
Mroh7	G	A	4: 106,548,184 (GRCm39)	A1098V	possibly damaging	Het
Mtrf1	G	A	14: 79,644,027 (GRCm39)	R174H	probably benign	Het
Mybpc3	G	A	2: 90,949,592 (GRCm39)	G45D	possibly damaging	Het
Mycbpap	A	T	11: 94,395,764 (GRCm39)	N733K	probably damaging	Het
N4bp1	T	C	8: 87,578,314 (GRCm39)	I684V	possibly damaging	Het
Nav2	A	T	7: 49,202,625 (GRCm39)	R1470*	probably null	Het
Ndufa9	A	T	6: 126,799,026 (GRCm39)	S364T	probably benign	Het
Nipal4	C	A	11: 46,052,837 (GRCm39)	A43S	possibly damaging	Het
Nlrp1a	T	A	11: 70,983,141 (GRCm39)	Y1275F	possibly damaging	Het
Nova1	A	T	12: 46,767,618 (GRCm39)	L8*	probably null	Het
Odam	G	T	5: 88,037,967 (GRCm39)	G181*	probably null	Het
Optn	T	A	2: 5,026,190 (GRCm39)	Q576L	probably benign	Het
Or2c1	T	C	16: 3,657,434 (GRCm39)	L199P	probably damaging	Het
Or4k47	T	C	2: 111,451,879 (GRCm39)	D180G	probably damaging	Het
Or4l1	C	T	14: 50,166,354 (GRCm39)	V216I	probably benign	Het
Or51a39	A	T	7: 102,362,702 (GRCm39)	I306N	probably damaging	Het
Or5as1	T	A	2: 86,981,003 (GRCm39)	M1L	possibly damaging	Het
Or5h18	A	T	16: 58,847,696 (GRCm39)	D191E	probably damaging	Het
Or8s2	T	C	15: 98,277,030 (GRCm39)		probably benign	Het
Patl2	G	T	2: 121,959,329 (GRCm39)	S45*	probably null	Het
Pde2a	G	A	7: 101,152,140 (GRCm39)	G349E	probably benign	Het
Pdlim2	T	A	14: 70,405,464 (GRCm39)		probably benign	Het
Per1	T	C	11: 68,995,227 (GRCm39)	V653A	probably benign	Het
Phlda2	A	G	7: 143,056,005 (GRCm39)	S75P	probably damaging	Het
Poldip3	A	T	15: 83,021,706 (GRCm39)	M167K	possibly damaging	Het
Potegl	C	A	2: 23,135,127 (GRCm39)	T312K	probably benign	Het
Prpf38a	T	C	4: 108,436,278 (GRCm39)	I12V	probably benign	Het
Prrc1	G	A	18: 57,507,622 (GRCm39)	V259I	possibly damaging	Het
Ptges3l	A	T	11: 101,315,448 (GRCm39)	M1K	probably null	Het
Rdh5	A	G	10: 128,749,653 (GRCm39)	Y296H	probably damaging	Het
Resf1	G	T	6: 149,229,896 (GRCm39)	G981*	probably null	Het
Rnf2	T	A	1: 151,348,968 (GRCm39)	K51*	probably null	Het
Rpusd3	C	A	6: 113,393,809 (GRCm39)	R215L	probably benign	Het
Rsph4a	A	T	10: 33,785,236 (GRCm39)	E382D	probably damaging	Het
S1pr2	G	A	9: 20,879,745 (GRCm39)	Q28*	probably null	Het
Sesn1	A	T	10: 41,771,005 (GRCm39)	I179F	probably damaging	Het
Setd3	T	A	12: 108,079,630 (GRCm39)	E291V	probably benign	Het
Shc1	G	T	3: 89,334,303 (GRCm39)	R323L	probably damaging	Het
Slc22a5	T	C	11: 53,782,352 (GRCm39)	D5G	possibly damaging	Het
Slc6a19	T	C	13: 73,848,677 (GRCm39)	K26E	probably benign	Het
Slc9a3	A	G	13: 74,312,412 (GRCm39)	N670D	possibly damaging	Het
Spata19	A	T	9: 27,311,761 (GRCm39)	I127L	probably benign	Het
Speg	G	T	1: 75,404,347 (GRCm39)	V2751L	probably damaging	Het
Sptbn2	A	G	19: 4,779,337 (GRCm39)	D298G	probably benign	Het
Srm	T	C	4: 148,678,640 (GRCm39)	V289A	possibly damaging	Het
Stip1	C	T	19: 7,012,938 (GRCm39)	A49T	probably benign	Het
Tas2r118	C	A	6: 23,969,627 (GRCm39)	V145F	probably benign	Het
Tbc1d12	A	T	19: 38,854,169 (GRCm39)	K284*	probably null	Het
Tfcp2	T	C	15: 100,423,531 (GRCm39)	H125R	probably damaging	Het
Tfdp1	T	C	8: 13,423,073 (GRCm39)	V206A	probably damaging	Het
Tgtp2	A	G	11: 48,950,237 (GRCm39)	W112R	probably damaging	Het
Tmem71	T	G	15: 66,410,710 (GRCm39)	M221L	probably benign	Het
Tpcn1	T	C	5: 120,685,552 (GRCm39)	N436S	possibly damaging	Het
Usp4	C	T	9: 108,239,819 (GRCm39)	L183F	probably damaging	Het
Vmn2r55	A	T	7: 12,404,478 (GRCm39)	N308K	possibly damaging	Het
Zfp106	T	A	2: 120,364,400 (GRCm39)	D669V	probably damaging	Het
Zfp108	A	T	7: 23,959,573 (GRCm39)	I55L	probably benign	Het
Zfp512b	A	T	2: 181,228,131 (GRCm39)	S8R	probably damaging	Het
Zfp827	T	C	8: 79,787,910 (GRCm39)	S359P	probably benign	Het

Other mutations in Helz2

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00089:Helz2	APN	2	180,871,495 (GRCm39)	missense	probably damaging	1.00
IGL00515:Helz2	APN	2	180,874,799 (GRCm39)	nonsense	probably null
IGL00704:Helz2	APN	2	180,876,178 (GRCm39)	missense	probably damaging	1.00
IGL00847:Helz2	APN	2	180,874,038 (GRCm39)	missense	possibly damaging	0.73
IGL01448:Helz2	APN	2	180,875,770 (GRCm39)	missense	probably damaging	1.00
IGL01783:Helz2	APN	2	180,874,674 (GRCm39)	missense	probably damaging	1.00
IGL01790:Helz2	APN	2	180,880,274 (GRCm39)	missense	probably benign	0.29
IGL02116:Helz2	APN	2	180,873,978 (GRCm39)	missense	probably damaging	1.00
IGL02226:Helz2	APN	2	180,873,483 (GRCm39)	missense	probably damaging	1.00
IGL02402:Helz2	APN	2	180,872,704 (GRCm39)	missense	probably damaging	1.00
IGL02403:Helz2	APN	2	180,872,815 (GRCm39)	missense	probably damaging	1.00
IGL02733:Helz2	APN	2	180,876,819 (GRCm39)	missense	probably benign	0.14
IGL02869:Helz2	APN	2	180,872,939 (GRCm39)	intron	probably benign
IGL03003:Helz2	APN	2	180,882,046 (GRCm39)	missense	probably damaging	1.00
IGL03060:Helz2	APN	2	180,871,015 (GRCm39)	critical splice donor site	probably null
IGL03310:Helz2	APN	2	180,873,597 (GRCm39)	missense	probably benign	0.00
Colby	UTSW	2	180,874,995 (GRCm39)	missense	probably damaging	1.00
ANU74:Helz2	UTSW	2	180,876,627 (GRCm39)	missense	probably benign	0.03
R0013:Helz2	UTSW	2	180,882,752 (GRCm39)	missense	probably benign
R0013:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	180,882,304 (GRCm39)	missense	probably damaging	1.00
R0014:Helz2	UTSW	2	180,882,304 (GRCm39)	missense	probably damaging	1.00
R0016:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0018:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0019:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0055:Helz2	UTSW	2	180,870,614 (GRCm39)	missense	possibly damaging	0.47
R0055:Helz2	UTSW	2	180,870,614 (GRCm39)	missense	possibly damaging	0.47
R0071:Helz2	UTSW	2	180,878,200 (GRCm39)	missense	probably damaging	1.00
R0071:Helz2	UTSW	2	180,878,200 (GRCm39)	missense	probably damaging	1.00
R0111:Helz2	UTSW	2	180,879,595 (GRCm39)	missense	probably benign	0.30
R0117:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0135:Helz2	UTSW	2	180,874,062 (GRCm39)	missense	probably damaging	1.00
R0194:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	180,872,223 (GRCm39)	missense	probably damaging	1.00
R0242:Helz2	UTSW	2	180,872,223 (GRCm39)	missense	probably damaging	1.00
R0254:Helz2	UTSW	2	180,874,552 (GRCm39)	missense	probably damaging	1.00
R0410:Helz2	UTSW	2	180,872,386 (GRCm39)	missense	probably damaging	1.00
R0442:Helz2	UTSW	2	180,874,002 (GRCm39)	missense	probably damaging	0.97
R0497:Helz2	UTSW	2	180,871,449 (GRCm39)	missense	probably damaging	0.97
R0517:Helz2	UTSW	2	180,869,563 (GRCm39)	missense	probably benign	0.00
R0541:Helz2	UTSW	2	180,876,618 (GRCm39)	missense	possibly damaging	0.89
R0542:Helz2	UTSW	2	180,873,882 (GRCm39)	missense	probably damaging	1.00
R0591:Helz2	UTSW	2	180,873,909 (GRCm39)	missense	probably damaging	0.96
R0692:Helz2	UTSW	2	180,882,674 (GRCm39)	missense	probably benign
R0826:Helz2	UTSW	2	180,882,646 (GRCm39)	missense	possibly damaging	0.51
R0834:Helz2	UTSW	2	180,872,570 (GRCm39)	missense	probably damaging	1.00
R0880:Helz2	UTSW	2	180,877,928 (GRCm39)	missense	probably benign
R1170:Helz2	UTSW	2	180,871,608 (GRCm39)	missense	probably damaging	1.00
R1186:Helz2	UTSW	2	180,872,921 (GRCm39)	missense	probably damaging	1.00
R1344:Helz2	UTSW	2	180,879,389 (GRCm39)	missense	possibly damaging	0.89
R1358:Helz2	UTSW	2	180,874,774 (GRCm39)	missense	probably damaging	1.00
R1436:Helz2	UTSW	2	180,877,317 (GRCm39)	missense	probably damaging	0.99
R1464:Helz2	UTSW	2	180,881,447 (GRCm39)	missense	probably damaging	1.00
R1464:Helz2	UTSW	2	180,881,447 (GRCm39)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	180,878,090 (GRCm39)	missense	probably damaging	1.00
R1466:Helz2	UTSW	2	180,878,090 (GRCm39)	missense	probably damaging	1.00
R1477:Helz2	UTSW	2	180,874,597 (GRCm39)	missense	probably benign	0.00
R1564:Helz2	UTSW	2	180,875,021 (GRCm39)	missense	probably benign	0.01
R1584:Helz2	UTSW	2	180,878,090 (GRCm39)	missense	probably damaging	1.00
R1655:Helz2	UTSW	2	180,875,940 (GRCm39)	missense	probably damaging	0.99
R1757:Helz2	UTSW	2	180,878,056 (GRCm39)	missense	probably damaging	1.00
R1779:Helz2	UTSW	2	180,880,252 (GRCm39)	missense	possibly damaging	0.84
R1779:Helz2	UTSW	2	180,876,780 (GRCm39)	missense	probably benign
R1837:Helz2	UTSW	2	180,871,082 (GRCm39)	missense	probably damaging	1.00
R1845:Helz2	UTSW	2	180,873,878 (GRCm39)	missense	probably benign	0.02
R1894:Helz2	UTSW	2	180,876,082 (GRCm39)	missense	probably damaging	1.00
R1913:Helz2	UTSW	2	180,875,543 (GRCm39)	missense	probably damaging	1.00
R2005:Helz2	UTSW	2	180,873,122 (GRCm39)	missense	probably benign	0.45
R2034:Helz2	UTSW	2	180,874,371 (GRCm39)	missense	probably damaging	1.00
R2036:Helz2	UTSW	2	180,879,272 (GRCm39)	missense	probably benign	0.03
R2061:Helz2	UTSW	2	180,882,337 (GRCm39)	missense	probably damaging	1.00
R2088:Helz2	UTSW	2	180,876,895 (GRCm39)	missense	probably benign	0.07
R2142:Helz2	UTSW	2	180,873,173 (GRCm39)	missense	probably benign
R2180:Helz2	UTSW	2	180,875,525 (GRCm39)	missense	probably damaging	1.00
R2192:Helz2	UTSW	2	180,870,841 (GRCm39)	nonsense	probably null
R2248:Helz2	UTSW	2	180,875,226 (GRCm39)	missense	probably benign	0.33
R2495:Helz2	UTSW	2	180,874,705 (GRCm39)	missense	probably damaging	0.99
R2886:Helz2	UTSW	2	180,882,535 (GRCm39)	missense	probably benign
R3617:Helz2	UTSW	2	180,874,854 (GRCm39)	missense	probably damaging	1.00
R3776:Helz2	UTSW	2	180,882,182 (GRCm39)	nonsense	probably null
R3803:Helz2	UTSW	2	180,881,789 (GRCm39)	missense	probably damaging	0.96
R4043:Helz2	UTSW	2	180,871,503 (GRCm39)	missense	probably benign	0.00
R4052:Helz2	UTSW	2	180,882,268 (GRCm39)	missense	probably damaging	1.00
R4232:Helz2	UTSW	2	180,871,695 (GRCm39)	missense	probably damaging	1.00
R4521:Helz2	UTSW	2	180,870,626 (GRCm39)	missense	probably benign
R4624:Helz2	UTSW	2	180,881,101 (GRCm39)	missense	probably damaging	0.99
R4720:Helz2	UTSW	2	180,880,210 (GRCm39)	missense	probably damaging	1.00
R4831:Helz2	UTSW	2	180,879,210 (GRCm39)	missense	probably damaging	1.00
R4852:Helz2	UTSW	2	180,871,913 (GRCm39)	missense	probably damaging	1.00
R4894:Helz2	UTSW	2	180,877,940 (GRCm39)	missense	probably benign	0.01
R4915:Helz2	UTSW	2	180,874,231 (GRCm39)	missense	possibly damaging	0.80
R5022:Helz2	UTSW	2	180,882,362 (GRCm39)	missense	probably benign
R5089:Helz2	UTSW	2	180,876,942 (GRCm39)	missense	probably benign	0.14
R5190:Helz2	UTSW	2	180,872,550 (GRCm39)	critical splice donor site	probably null
R5309:Helz2	UTSW	2	180,876,639 (GRCm39)	missense	probably benign	0.08
R5358:Helz2	UTSW	2	180,877,321 (GRCm39)	missense	probably damaging	1.00
R5379:Helz2	UTSW	2	180,876,862 (GRCm39)	missense	probably benign
R5559:Helz2	UTSW	2	180,871,919 (GRCm39)	missense	probably damaging	0.98
R5591:Helz2	UTSW	2	180,882,051 (GRCm39)	missense	probably damaging	0.99
R5596:Helz2	UTSW	2	180,879,082 (GRCm39)	intron	probably benign
R5805:Helz2	UTSW	2	180,882,301 (GRCm39)	missense	probably damaging	1.00
R5823:Helz2	UTSW	2	180,878,189 (GRCm39)	missense	possibly damaging	0.92
R5825:Helz2	UTSW	2	180,874,449 (GRCm39)	missense	probably benign	0.02
R5873:Helz2	UTSW	2	180,875,821 (GRCm39)	missense	possibly damaging	0.78
R5928:Helz2	UTSW	2	180,872,177 (GRCm39)	missense	possibly damaging	0.82
R5936:Helz2	UTSW	2	180,872,560 (GRCm39)	missense	probably damaging	1.00
R5975:Helz2	UTSW	2	180,872,843 (GRCm39)	missense	probably benign	0.08
R6045:Helz2	UTSW	2	180,882,106 (GRCm39)	missense	probably benign	0.03
R6077:Helz2	UTSW	2	180,874,831 (GRCm39)	missense	probably benign	0.41
R6218:Helz2	UTSW	2	180,874,087 (GRCm39)	missense	probably benign	0.03
R6218:Helz2	UTSW	2	180,877,738 (GRCm39)	missense	probably damaging	1.00
R6315:Helz2	UTSW	2	180,874,995 (GRCm39)	missense	probably damaging	1.00
R6346:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6371:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6372:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6373:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6385:Helz2	UTSW	2	180,875,260 (GRCm39)	missense	probably damaging	1.00
R6464:Helz2	UTSW	2	180,876,862 (GRCm39)	missense	probably benign
R6581:Helz2	UTSW	2	180,871,172 (GRCm39)	missense	probably damaging	0.99
R6651:Helz2	UTSW	2	180,881,350 (GRCm39)	nonsense	probably null
R6964:Helz2	UTSW	2	180,872,221 (GRCm39)	missense	probably damaging	1.00
R7061:Helz2	UTSW	2	180,882,307 (GRCm39)	missense	probably damaging	1.00
R7153:Helz2	UTSW	2	180,873,078 (GRCm39)	missense	probably benign	0.00
R7372:Helz2	UTSW	2	180,880,216 (GRCm39)	missense	possibly damaging	0.61
R7512:Helz2	UTSW	2	180,877,393 (GRCm39)	splice site	probably null
R7512:Helz2	UTSW	2	180,872,647 (GRCm39)	missense	probably benign	0.00
R7583:Helz2	UTSW	2	180,879,365 (GRCm39)	missense	probably benign	0.06
R7724:Helz2	UTSW	2	180,873,789 (GRCm39)	missense	probably damaging	1.00
R7733:Helz2	UTSW	2	180,872,148 (GRCm39)	missense	possibly damaging	0.63
R7748:Helz2	UTSW	2	180,876,324 (GRCm39)	missense	probably damaging	1.00
R7774:Helz2	UTSW	2	180,875,784 (GRCm39)	missense	probably benign
R7799:Helz2	UTSW	2	180,879,782 (GRCm39)	missense	probably benign	0.15
R7841:Helz2	UTSW	2	180,874,695 (GRCm39)	missense	probably damaging	1.00
R7939:Helz2	UTSW	2	180,879,543 (GRCm39)	missense	probably damaging	0.99
R8026:Helz2	UTSW	2	180,881,998 (GRCm39)	missense	probably benign	0.34
R8030:Helz2	UTSW	2	180,879,689 (GRCm39)	missense	possibly damaging	0.55
R8080:Helz2	UTSW	2	180,880,055 (GRCm39)	missense	probably damaging	0.99
R8237:Helz2	UTSW	2	180,871,124 (GRCm39)	missense	possibly damaging	0.65
R8245:Helz2	UTSW	2	180,879,895 (GRCm39)	missense	probably damaging	1.00
R8304:Helz2	UTSW	2	180,871,950 (GRCm39)	missense	probably benign	0.03
R8486:Helz2	UTSW	2	180,871,124 (GRCm39)	missense	probably damaging	1.00
R8556:Helz2	UTSW	2	180,871,350 (GRCm39)	missense	probably damaging	1.00
R8878:Helz2	UTSW	2	180,874,560 (GRCm39)	missense	possibly damaging	0.67
R8907:Helz2	UTSW	2	180,874,920 (GRCm39)	missense	possibly damaging	0.47
R8911:Helz2	UTSW	2	180,880,173 (GRCm39)	missense
R8953:Helz2	UTSW	2	180,874,884 (GRCm39)	missense	probably damaging	1.00
R8963:Helz2	UTSW	2	180,871,407 (GRCm39)	missense	probably damaging	1.00
R8969:Helz2	UTSW	2	180,879,581 (GRCm39)	missense	probably benign	0.19
R8976:Helz2	UTSW	2	180,876,486 (GRCm39)	missense	possibly damaging	0.46
R9015:Helz2	UTSW	2	180,870,792 (GRCm39)	missense	probably damaging	1.00
R9031:Helz2	UTSW	2	180,874,261 (GRCm39)	missense	possibly damaging	0.78
R9052:Helz2	UTSW	2	180,881,968 (GRCm39)	missense	possibly damaging	0.78
R9089:Helz2	UTSW	2	180,881,433 (GRCm39)	missense	probably damaging	1.00
R9145:Helz2	UTSW	2	180,881,848 (GRCm39)	missense	probably damaging	1.00
R9185:Helz2	UTSW	2	180,871,883 (GRCm39)	missense	probably benign
R9186:Helz2	UTSW	2	180,876,457 (GRCm39)	missense	possibly damaging	0.57
R9373:Helz2	UTSW	2	180,882,741 (GRCm39)	missense	probably benign
R9407:Helz2	UTSW	2	180,881,975 (GRCm39)	missense	probably benign	0.01
R9465:Helz2	UTSW	2	180,874,710 (GRCm39)	missense	probably benign	0.01
R9502:Helz2	UTSW	2	180,878,245 (GRCm39)	missense	possibly damaging	0.47
R9538:Helz2	UTSW	2	180,882,014 (GRCm39)	missense	probably damaging	1.00
R9554:Helz2	UTSW	2	180,882,470 (GRCm39)	missense	probably damaging	0.96
R9659:Helz2	UTSW	2	180,882,025 (GRCm39)	missense	probably benign	0.00
R9800:Helz2	UTSW	2	180,882,616 (GRCm39)	missense	probably damaging	0.99
X0064:Helz2	UTSW	2	180,873,534 (GRCm39)	missense	probably damaging	1.00
Z1176:Helz2	UTSW	2	180,879,357 (GRCm39)	missense	probably benign	0.39
Z1177:Helz2	UTSW	2	180,877,754 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- ATAGTAGTGACAGACTTGATAGCGC -3'
(R):5'- TTGACCTTGGGCTGACTTGC -3'

Sequencing Primer
(F):5'- CAGACTTGATAGCGCATAGGC -3'
(R):5'- GGGCTGACTTGCTTCTCTTGTC -3'

Posted On

2016-04-27