Incidental Mutation 'R4778:Nmd3'
| ID |
368073 |
| Institutional Source |
Beutler Lab
|
| Gene Symbol |
Nmd3
|
| Ensembl Gene |
ENSMUSG00000027787 |
| Gene Name |
NMD3 ribosome export adaptor |
| Synonyms |
C87860 |
| MMRRC Submission |
042414-MU
|
| Accession Numbers |
|
| Essential gene? |
Probably essential
(E-score: 0.965)
|
| Stock # |
R4778 (G1)
|
| Quality Score |
225 |
|
Status
|
Validated
|
| Chromosome |
3 |
| Chromosomal Location |
69629354-69656380 bp(+) (GRCm39) |
| Type of Mutation |
nonsense |
| DNA Base Change (assembly) |
T to A
at 69638924 bp (GRCm39)
|
| Zygosity |
Heterozygous |
| Amino Acid Change |
Tyrosine to Stop codon
at position 171
(Y171*)
|
| Ref Sequence |
ENSEMBL: ENSMUSP00000029358
(fasta)
|
| Gene Model |
predicted gene model for transcript(s):
[ENSMUST00000029358]
[ENSMUST00000135266]
[ENSMUST00000143041]
|
| AlphaFold |
Q99L48 |
| Predicted Effect |
probably null
Transcript: ENSMUST00000029358
AA Change: Y171*
|
| SMART Domains |
Protein: ENSMUSP00000029358
Gene: ENSMUSG00000027787
AA Change: Y171*
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:NMD3
|
17 |
246 |
6.6e-82 |
PFAM |
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000127211
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000135266
|
| SMART Domains |
Protein: ENSMUSP00000142290
Gene: ENSMUSG00000027787
| Domain | Start | End | E-Value | Type |
|---|
|
Pfam:NMD3
|
17 |
128 |
2e-31 |
PFAM |
|
| Predicted Effect |
probably benign
Transcript: ENSMUST00000143041
|
| Predicted Effect |
noncoding transcript
Transcript: ENSMUST00000194168
|
| Meta Mutation Damage Score |
0.9755 |
| Coding Region Coverage |
- 1x: 99.1%
- 3x: 98.5%
- 10x: 96.9%
- 20x: 94.3%
|
| Validation Efficiency |
98% (54/55) |
| MGI Phenotype |
FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] Ribosomal 40S and 60S subunits associate in the nucleolus and are exported to the cytoplasm. The protein encoded by this gene is involved in the passage of the 60S subunit through the nuclear pore complex and into the cytoplasm. Several transcript variants exist for this gene, but the full-length natures of only two have been described to date. [provided by RefSeq, Feb 2016]
|
| Allele List at MGI |
|
| Other mutations in this stock |
Total: 48 list
| Gene | Ref | Var | Chr/Loc | Mutation | Predicted Effect | Zygosity |
|---|
| Abcc10 |
T |
C |
17: 46,615,342 (GRCm39) |
N1349S |
probably damaging |
Het |
| Ahnak |
T |
C |
19: 8,989,339 (GRCm39) |
V3541A |
possibly damaging |
Het |
| Arhgap33 |
T |
A |
7: 30,231,518 (GRCm39) |
T156S |
probably benign |
Het |
| Bltp1 |
T |
A |
3: 36,991,214 (GRCm39) |
M897K |
possibly damaging |
Het |
| Card11 |
G |
T |
5: 140,869,537 (GRCm39) |
|
probably null |
Het |
| Cdh3 |
T |
A |
8: 107,270,458 (GRCm39) |
I445N |
probably damaging |
Het |
| Csrp3 |
T |
G |
7: 48,482,311 (GRCm39) |
K169N |
probably damaging |
Het |
| Cyp2c69 |
T |
C |
19: 39,866,038 (GRCm39) |
N185S |
probably benign |
Het |
| Czib |
T |
C |
4: 107,749,195 (GRCm39) |
V64A |
probably damaging |
Het |
| Dpysl3 |
C |
T |
18: 43,487,867 (GRCm39) |
V159I |
probably benign |
Het |
| Erc1 |
A |
T |
6: 119,774,298 (GRCm39) |
|
probably null |
Het |
| Fat1 |
T |
C |
8: 45,491,363 (GRCm39) |
V3808A |
probably benign |
Het |
| Fbxw19 |
T |
C |
9: 109,323,714 (GRCm39) |
D87G |
probably damaging |
Het |
| Gm1123 |
T |
C |
9: 98,900,560 (GRCm39) |
I99V |
probably benign |
Het |
| Gm11677 |
C |
T |
11: 111,615,537 (GRCm39) |
|
noncoding transcript |
Het |
| Hand1 |
T |
C |
11: 57,722,449 (GRCm39) |
D55G |
possibly damaging |
Het |
| Lrguk |
T |
A |
6: 34,033,015 (GRCm39) |
I227K |
probably damaging |
Het |
| Mdn1 |
C |
T |
4: 32,683,583 (GRCm39) |
R726* |
probably null |
Het |
| Minar1 |
T |
A |
9: 89,485,155 (GRCm39) |
I81F |
probably damaging |
Het |
| Myo16 |
T |
A |
8: 10,619,694 (GRCm39) |
V1415E |
probably damaging |
Het |
| Myof |
T |
C |
19: 37,938,011 (GRCm39) |
D901G |
probably damaging |
Het |
| Naip1 |
A |
G |
13: 100,563,156 (GRCm39) |
Y670H |
probably damaging |
Het |
| Notch4 |
C |
T |
17: 34,801,485 (GRCm39) |
A1111V |
possibly damaging |
Het |
| Nphp4 |
T |
A |
4: 152,640,748 (GRCm39) |
D1038E |
probably benign |
Het |
| Or2ag1b |
A |
C |
7: 106,288,874 (GRCm39) |
S21R |
probably damaging |
Het |
| Or5p60 |
A |
T |
7: 107,723,687 (GRCm39) |
I261N |
possibly damaging |
Het |
| Osbpl10 |
C |
T |
9: 114,938,598 (GRCm39) |
S86L |
probably damaging |
Het |
| Pcsk6 |
G |
A |
7: 65,608,893 (GRCm39) |
G252R |
probably damaging |
Het |
| Pole |
T |
G |
5: 110,478,698 (GRCm39) |
H15Q |
probably benign |
Het |
| Pstpip1 |
A |
G |
9: 56,035,904 (GRCm39) |
D383G |
possibly damaging |
Het |
| Ptprq |
T |
C |
10: 107,426,883 (GRCm39) |
T1551A |
probably benign |
Het |
| Rasgrf2 |
A |
G |
13: 92,131,780 (GRCm39) |
F626L |
probably damaging |
Het |
| Retreg1 |
C |
A |
15: 25,971,871 (GRCm39) |
N394K |
possibly damaging |
Het |
| Rpl7-ps8 |
T |
A |
15: 59,083,252 (GRCm39) |
|
noncoding transcript |
Het |
| Rpp14 |
T |
A |
14: 8,090,203 (GRCm38) |
D42E |
probably benign |
Het |
| Rrp8 |
T |
A |
7: 105,386,481 (GRCm39) |
|
probably benign |
Het |
| Rufy4 |
T |
C |
1: 74,186,822 (GRCm39) |
C537R |
probably damaging |
Het |
| Snx1 |
T |
C |
9: 66,008,698 (GRCm39) |
|
probably benign |
Het |
| Stau1 |
A |
T |
2: 166,805,442 (GRCm39) |
N51K |
probably benign |
Het |
| Tdrd5 |
T |
A |
1: 156,083,157 (GRCm39) |
D960V |
probably damaging |
Het |
| Tex10 |
T |
C |
4: 48,436,468 (GRCm39) |
D750G |
probably damaging |
Het |
| Tmem43 |
T |
C |
6: 91,459,237 (GRCm39) |
V236A |
probably damaging |
Het |
| Tmem89 |
T |
C |
9: 108,744,443 (GRCm39) |
V112A |
probably damaging |
Het |
| Traf7 |
C |
G |
17: 24,729,412 (GRCm39) |
|
probably benign |
Het |
| Vmn1r41 |
A |
C |
6: 89,724,257 (GRCm39) |
K266T |
probably damaging |
Het |
| Vmn2r65 |
T |
C |
7: 84,592,801 (GRCm39) |
K469E |
possibly damaging |
Het |
| Zfp831 |
A |
G |
2: 174,488,600 (GRCm39) |
T1092A |
possibly damaging |
Het |
| Zfp981 |
T |
A |
4: 146,622,112 (GRCm39) |
S346T |
probably benign |
Het |
|
| Other mutations in Nmd3 |
| Allele | Source | Chr | Coord | Type | Predicted Effect | PPH Score |
|---|
|
IGL00402:Nmd3
|
APN |
3 |
69,652,573 (GRCm39) |
missense |
possibly damaging |
0.63 |
|
IGL01014:Nmd3
|
APN |
3 |
69,633,719 (GRCm39) |
missense |
probably benign |
0.00 |
|
IGL01289:Nmd3
|
APN |
3 |
69,631,620 (GRCm39) |
missense |
possibly damaging |
0.85 |
|
IGL02566:Nmd3
|
APN |
3 |
69,647,247 (GRCm39) |
unclassified |
probably benign |
|
|
IGL03259:Nmd3
|
APN |
3 |
69,652,576 (GRCm39) |
missense |
possibly damaging |
0.49 |
|
IGL03299:Nmd3
|
APN |
3 |
69,637,762 (GRCm39) |
splice site |
probably null |
|
|
IGL03382:Nmd3
|
APN |
3 |
69,642,421 (GRCm39) |
missense |
probably damaging |
0.99 |
|
R0017:Nmd3
|
UTSW |
3 |
69,643,425 (GRCm39) |
splice site |
probably null |
|
|
R0025:Nmd3
|
UTSW |
3 |
69,655,654 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R0350:Nmd3
|
UTSW |
3 |
69,650,907 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R1136:Nmd3
|
UTSW |
3 |
69,654,049 (GRCm39) |
splice site |
probably benign |
|
|
R1635:Nmd3
|
UTSW |
3 |
69,647,317 (GRCm39) |
missense |
probably benign |
0.03 |
|
R3081:Nmd3
|
UTSW |
3 |
69,631,732 (GRCm39) |
splice site |
probably benign |
|
|
R3686:Nmd3
|
UTSW |
3 |
69,654,095 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R3758:Nmd3
|
UTSW |
3 |
69,631,641 (GRCm39) |
nonsense |
probably null |
|
|
R4384:Nmd3
|
UTSW |
3 |
69,631,731 (GRCm39) |
splice site |
probably benign |
|
|
R4774:Nmd3
|
UTSW |
3 |
69,652,569 (GRCm39) |
missense |
probably benign |
0.11 |
|
R4953:Nmd3
|
UTSW |
3 |
69,638,970 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R5000:Nmd3
|
UTSW |
3 |
69,624,735 (GRCm39) |
unclassified |
probably benign |
|
|
R5182:Nmd3
|
UTSW |
3 |
69,629,801 (GRCm39) |
critical splice donor site |
probably null |
|
|
R6043:Nmd3
|
UTSW |
3 |
69,652,580 (GRCm39) |
missense |
probably benign |
|
|
R6355:Nmd3
|
UTSW |
3 |
69,636,680 (GRCm39) |
missense |
probably benign |
0.22 |
|
R6760:Nmd3
|
UTSW |
3 |
69,654,170 (GRCm39) |
critical splice donor site |
probably null |
|
|
R7869:Nmd3
|
UTSW |
3 |
69,633,750 (GRCm39) |
missense |
probably damaging |
1.00 |
|
R8024:Nmd3
|
UTSW |
3 |
69,637,298 (GRCm39) |
unclassified |
probably benign |
|
|
R8729:Nmd3
|
UTSW |
3 |
69,655,682 (GRCm39) |
missense |
possibly damaging |
0.88 |
|
R9018:Nmd3
|
UTSW |
3 |
69,647,328 (GRCm39) |
missense |
probably benign |
0.08 |
|
R9419:Nmd3
|
UTSW |
3 |
69,643,349 (GRCm39) |
missense |
probably benign |
0.14 |
|
R9499:Nmd3
|
UTSW |
3 |
69,647,329 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
R9551:Nmd3
|
UTSW |
3 |
69,647,329 (GRCm39) |
missense |
possibly damaging |
0.92 |
|
| Predicted Primers |
PCR Primer
(F):5'- GGAGTCTAGGTTCCTGGAACATTC -3'
(R):5'- GAGTAGCTAGCTATACTTCAGTCAG -3'
Sequencing Primer
(F):5'- GGTTCCTGGAACATTCAACTTTAG -3'
(R):5'- CTGGAACTCACTCTGTAGATCAGG -3'
|
| Posted On |
2015-12-29 |
Incidental Mutation