Mutagenetix > Incidental Mutation

Incidental Mutation 'R4492:Irgm1'

330793

Institutional Source

Beutler Lab

Gene Symbol

Irgm1

Ensembl Gene

ENSMUSG00000046879

Gene Name

immunity-related GTPase family M member 1

Synonyms

Iigp3, Irgm, Ifi1, LRG-47

MMRRC Submission

041581-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R4492 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

48756072-48762247 bp(-) (GRCm39)

Type of Mutation

synonymous

DNA Base Change (assembly)

G to A at 48756955 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Ref Sequence

ENSEMBL: ENSMUSP00000094870 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000049519] [ENSMUST00000097271] [ENSMUST00000097271]

AlphaFold

Q60766

Predicted Effect

silent
Transcript: ENSMUST00000049519

SMART Domains

Protein: ENSMUSP00000050446
Gene: ENSMUSG00000046879

Domain	Start	End	E-Value	Type
Pfam:IIGP	42	399	5.5e-169	PFAM

Predicted Effect

silent
Transcript: ENSMUST00000097271

SMART Domains

Protein: ENSMUSP00000094870
Gene: ENSMUSG00000046879

Domain	Start	End	E-Value	Type
Pfam:IIGP	26	288	2.5e-131	PFAM
Pfam:MMR_HSR1	62	175	2.8e-6	PFAM

Predicted Effect

silent
Transcript: ENSMUST00000097271

SMART Domains

Protein: ENSMUSP00000094870
Gene: ENSMUSG00000046879

Domain	Start	End	E-Value	Type
Pfam:IIGP	26	288	2.5e-131	PFAM
Pfam:MMR_HSR1	62	175	2.8e-6	PFAM

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000147151

Meta Mutation Damage Score

0.0869

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.1%
20x: 94.9%

Validation Efficiency

MGI Phenotype

PHENOTYPE: In the absence of infection, mice show no overt phenotype. Resistance to protozoan parasitic infection and bacterial infection is impaired in homozygous mutant mice, whereas resistance to viral infection is normal. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 48 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Actr6	T	G	10: 89,561,676 (GRCm39)	I157L	probably benign	Het
Amph	T	C	13: 19,333,928 (GRCm39)	V663A	possibly damaging	Het
Anapc16	A	G	10: 59,826,724 (GRCm39)	S50P	possibly damaging	Het
Ank3	T	A	10: 69,644,755 (GRCm39)	V60D	probably damaging	Het
Btbd9	A	G	17: 30,746,545 (GRCm39)	Y94H	probably damaging	Het
Chil3	A	G	3: 106,063,017 (GRCm39)	I191T	probably damaging	Het
Clpb	T	C	7: 101,436,929 (GRCm39)	L668P	probably damaging	Het
Cyp2d22	G	A	15: 82,258,571 (GRCm39)	H97Y	probably benign	Het
Dhrs7	A	T	12: 72,699,899 (GRCm39)	N244K	probably damaging	Het
Dusp4	C	T	8: 35,274,890 (GRCm39)	T3M	possibly damaging	Het
Edc4	GGATTTTAGCCA	G	8: 106,611,700 (GRCm39)		probably null	Het
Etl4	G	A	2: 20,811,676 (GRCm39)	S1621N	possibly damaging	Het
Fam174a	T	C	1: 95,241,701 (GRCm39)	S54P	probably benign	Het
Fdxacb1	T	C	9: 50,681,547 (GRCm39)	F7S	probably damaging	Het
Focad	G	A	4: 88,278,142 (GRCm39)		probably null	Het
Gpr156	T	A	16: 37,812,468 (GRCm39)	L268H	probably damaging	Het
Gpr17	A	T	18: 32,080,304 (GRCm39)	I253N	possibly damaging	Het
H2-T23	A	T	17: 36,343,058 (GRCm39)	N106K	probably damaging	Het
Hspa4	T	C	11: 53,171,296 (GRCm39)	R303G	probably damaging	Het
Jph1	A	C	1: 17,067,770 (GRCm39)	I114S	probably damaging	Het
Kcna1	C	T	6: 126,619,238 (GRCm39)	D361N	possibly damaging	Het
Kcna4	G	A	2: 107,126,436 (GRCm39)	R390Q	probably damaging	Het
Lum	C	A	10: 97,404,300 (GRCm39)	P65H	probably damaging	Het
Mc4r	A	G	18: 66,992,711 (GRCm39)	L134P	probably benign	Het
Mroh8	A	T	2: 157,099,960 (GRCm39)	I248N	probably damaging	Het
Nos2	A	G	11: 78,840,921 (GRCm39)	T677A	probably benign	Het
Nup37	T	A	10: 88,010,791 (GRCm39)	F257I	possibly damaging	Het
Or13c3	T	C	4: 52,855,764 (GRCm39)	I250V	probably benign	Het
Or6k6	C	T	1: 173,944,770 (GRCm39)	V271I	probably benign	Het
Pdzd2	C	T	15: 12,385,723 (GRCm39)	D1016N	possibly damaging	Het
Pdzd2	A	C	15: 12,419,567 (GRCm39)	M501R	possibly damaging	Het
Pitx1	T	C	13: 55,976,465 (GRCm39)	K65E	probably benign	Het
Pla1a	C	T	16: 38,229,972 (GRCm39)	A247T	probably benign	Het
Prex2	T	A	1: 11,232,487 (GRCm39)	S851R	probably benign	Het
Prss8	A	G	7: 127,528,979 (GRCm39)	S26P	probably damaging	Het
Rasef	A	C	4: 73,652,740 (GRCm39)	L587R	probably damaging	Het
Rock2	T	A	12: 17,027,684 (GRCm39)	C1334S	probably damaging	Het
Rsf1	CGGCGGCGG	CGGCGGCGGGGGCGGCGG	7: 97,229,130 (GRCm39)		probably benign	Het
Serpina6	A	G	12: 103,613,146 (GRCm39)	W385R	probably damaging	Het
Slc12a5	T	C	2: 164,821,263 (GRCm39)	M249T	probably benign	Het
Srsf9	T	A	5: 115,470,651 (GRCm39)	I117N	probably damaging	Het
Taf1	G	T	X: 100,586,665 (GRCm39)	M313I	possibly damaging	Het
Tmem30a	T	C	9: 79,684,567 (GRCm39)	H95R	probably damaging	Het
Top2b	T	G	14: 16,409,189 (GRCm38)	I777M	probably damaging	Het
Ttc21a	T	C	9: 119,770,346 (GRCm39)	V139A	probably benign	Het
Zfp236	A	T	18: 82,648,125 (GRCm39)	V1012D	probably damaging	Het
Zfp612	C	A	8: 110,815,929 (GRCm39)	Q379K	probably damaging	Het
Zfp930	C	T	8: 69,680,898 (GRCm39)	Q198*	probably null	Het

Other mutations in Irgm1

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00418:Irgm1	APN	11	48,756,832 (GRCm39)	nonsense	probably null
IGL01821:Irgm1	APN	11	48,757,353 (GRCm39)	missense	probably damaging	1.00
IGL02043:Irgm1	APN	11	48,757,642 (GRCm39)	missense	probably damaging	0.98
IGL02252:Irgm1	APN	11	48,756,981 (GRCm39)	missense	possibly damaging	0.92
IGL03153:Irgm1	APN	11	48,757,094 (GRCm39)	missense	probably damaging	1.00
igraine	UTSW	11	48,757,440 (GRCm39)	missense	probably benign	0.33
R0487:Irgm1	UTSW	11	48,757,154 (GRCm39)	missense	probably damaging	0.98
R1808:Irgm1	UTSW	11	48,757,259 (GRCm39)	missense	probably damaging	0.99
R1809:Irgm1	UTSW	11	48,757,440 (GRCm39)	missense	probably benign	0.33
R1878:Irgm1	UTSW	11	48,756,897 (GRCm39)	missense	probably benign	0.07
R2971:Irgm1	UTSW	11	48,757,417 (GRCm39)	nonsense	probably null
R4962:Irgm1	UTSW	11	48,757,159 (GRCm39)	missense	possibly damaging	0.93
R5186:Irgm1	UTSW	11	48,757,044 (GRCm39)	missense	probably benign	0.00
R5794:Irgm1	UTSW	11	48,757,064 (GRCm39)	missense	probably damaging	0.99
R6224:Irgm1	UTSW	11	48,757,713 (GRCm39)	missense	probably benign	0.02
R6487:Irgm1	UTSW	11	48,756,777 (GRCm39)	missense	probably benign	0.21
R6752:Irgm1	UTSW	11	48,757,290 (GRCm39)	missense	probably damaging	1.00
R6766:Irgm1	UTSW	11	48,756,928 (GRCm39)	missense	possibly damaging	0.80
R8005:Irgm1	UTSW	11	48,757,217 (GRCm39)	missense	probably damaging	1.00
R8418:Irgm1	UTSW	11	48,757,166 (GRCm39)	missense	probably damaging	0.97
R8924:Irgm1	UTSW	11	48,756,698 (GRCm39)	missense	probably benign	0.35
R8947:Irgm1	UTSW	11	48,759,575 (GRCm39)	intron	probably benign

Predicted Primers

PCR Primer
(F):5'- TACGCATGGGAGAAATCTCAAC -3'
(R):5'- TGACTTCCCGAAGCTTAGGG -3'

Sequencing Primer
(F):5'- CGGAAGAATGCATTCACAATTGC -3'
(R):5'- GCTTAGGGACACACTTCATAAAG -3'

Posted On

2015-07-21