Mutagenetix > Incidental Mutation

Incidental Mutation 'R0394:Vsig10l'

31859

Institutional Source

Beutler Lab

Gene Symbol

Vsig10l

Ensembl Gene

ENSMUSG00000070604

Gene Name

V-set and immunoglobulin domain containing 10 like

Synonyms

2210412E05Rik

MMRRC Submission

038600-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.197) question?

Stock #

R0394 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

43112575-43121443 bp(+) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 43114879 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Asparagine to Serine at position 360 (N360S)

Ref Sequence

ENSEMBL: ENSMUSP00000144692 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000107977] [ENSMUST00000203042] [ENSMUST00000203633] [ENSMUST00000203769] [ENSMUST00000204680]

AlphaFold

D3YZF7

Predicted Effect

possibly damaging
Transcript: ENSMUST00000107977
AA Change: N462S

PolyPhen 2 Score 0.707 (Sensitivity: 0.86; Specificity: 0.92)

SMART Domains

Protein: ENSMUSP00000103611
Gene: ENSMUSG00000070604
AA Change: N462S

Domain	Start	End	E-Value	Type
signal peptide	1	21	N/A	INTRINSIC
low complexity region	28	52	N/A	INTRINSIC
IG	183	285	1.92e0	SMART
IG	298	383	2.15e-3	SMART
IGc2	406	465	4.19e-6	SMART
Blast:IG_like	582	649	1e-13	BLAST
transmembrane domain	764	786	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000203042

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000203152

Predicted Effect

probably benign
Transcript: ENSMUST00000203633

SMART Domains

Protein: ENSMUSP00000145061
Gene: ENSMUSG00000107482

Domain	Start	End	E-Value	Type
ETF	26	216	2.7e-65	SMART

Predicted Effect

probably damaging
Transcript: ENSMUST00000203769
AA Change: N360S

PolyPhen 2 Score 0.967 (Sensitivity: 0.77; Specificity: 0.95)

SMART Domains

Protein: ENSMUSP00000144692
Gene: ENSMUSG00000070604
AA Change: N360S

Domain	Start	End	E-Value	Type
IG	81	183	8.1e-3	SMART
IG	196	281	9.2e-6	SMART
IGc2	304	363	1.8e-8	SMART
Blast:IG_like	480	547	9e-14	BLAST
transmembrane domain	662	684	N/A	INTRINSIC

Predicted Effect

probably benign
Transcript: ENSMUST00000204680

SMART Domains

Protein: ENSMUSP00000145493
Gene: ENSMUSG00000107482

Domain	Start	End	E-Value	Type
ETF	26	168	2.4e-17	SMART

Predicted Effect

noncoding transcript
Transcript: ENSMUST00000205247

Meta Mutation Damage Score

0.3924

Coding Region Coverage

1x: 99.1%
3x: 98.2%
10x: 96.0%
20x: 92.0%

Validation Efficiency

99% (79/80)

Allele List at MGI

Other mutations in this stock

Total: 78 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
1110002E22Rik	C	A	3: 137,773,065 (GRCm39)	S751R	probably damaging	Het
Abca8a	A	G	11: 109,917,169 (GRCm39)	V1610A	probably damaging	Het
Actl10	A	T	2: 154,394,957 (GRCm39)	H202L	probably benign	Het
Alox12	A	T	11: 70,136,761 (GRCm39)	V489E	probably damaging	Het
Ap4m1	T	A	5: 138,170,465 (GRCm39)	F5I	probably benign	Het
Atn1	T	C	6: 124,726,696 (GRCm39)		probably benign	Het
Atrnl1	T	A	19: 57,661,608 (GRCm39)	N529K	probably benign	Het
B3gntl1	C	T	11: 121,510,541 (GRCm39)	G336D	probably damaging	Het
Bmp1	G	A	14: 70,727,474 (GRCm39)	A703V	probably damaging	Het
Brat1	T	G	5: 140,704,141 (GRCm39)	L798R	probably damaging	Het
Cacna1c	C	T	6: 118,602,458 (GRCm39)	G1302R	probably damaging	Het
Cdr2	A	T	7: 120,557,954 (GRCm39)	D190E	probably benign	Het
Cenpe	T	C	3: 134,922,186 (GRCm39)		probably benign	Het
Clstn1	A	G	4: 149,728,635 (GRCm39)	D687G	probably benign	Het
Coro1a	A	G	7: 126,299,812 (GRCm39)	F337L	probably benign	Het
Ddx49	T	A	8: 70,749,575 (GRCm39)	I252F	probably damaging	Het
Dennd2a	T	A	6: 39,499,746 (GRCm39)	D273V	possibly damaging	Het
Derl2	A	T	11: 70,905,387 (GRCm39)	F32I	probably benign	Het
Dmrta1	A	G	4: 89,580,276 (GRCm39)	Y412C	probably damaging	Het
Dsg1a	A	G	18: 20,466,807 (GRCm39)	N559S	probably damaging	Het
Dusp26	G	T	8: 31,581,987 (GRCm39)	R27L	probably benign	Het
Eif2ak3	T	C	6: 70,862,202 (GRCm39)	I492T	probably benign	Het
Exoc7	G	T	11: 116,191,224 (GRCm39)	Q219K	probably damaging	Het
F2r	T	C	13: 95,740,984 (GRCm39)	T184A	probably damaging	Het
Fbf1	G	A	11: 116,043,288 (GRCm39)		probably benign	Het
Fbxo28	A	G	1: 182,144,580 (GRCm39)	M328T	probably benign	Het
Fsip2	T	A	2: 82,821,419 (GRCm39)	D5717E	possibly damaging	Het
Gnpat	C	A	8: 125,606,964 (GRCm39)	S373R	possibly damaging	Het
Golgb1	G	T	16: 36,695,941 (GRCm39)		probably benign	Het
Greb1l	T	C	18: 10,523,374 (GRCm39)	V844A	probably damaging	Het
Hps1	G	T	19: 42,759,338 (GRCm39)		probably null	Het
Inppl1	G	T	7: 101,477,402 (GRCm39)		probably benign	Het
Isca1	C	T	13: 59,906,699 (GRCm39)		probably null	Het
Itgb2	T	A	10: 77,378,309 (GRCm39)	C46S	probably damaging	Het
Kifc5b	C	T	17: 27,142,056 (GRCm39)	T178M	probably benign	Het
Krt80	T	C	15: 101,250,180 (GRCm39)	T22A	probably damaging	Het
L3mbtl2	C	T	15: 81,552,942 (GRCm39)	A125V	probably damaging	Het
Ltbp2	C	T	12: 84,853,198 (GRCm39)		probably benign	Het
Mettl18	A	G	1: 163,823,910 (GRCm39)	D77G	probably benign	Het
Mfsd2a	A	G	4: 122,843,961 (GRCm39)	L336P	probably benign	Het
Mgat4b	A	G	11: 50,121,746 (GRCm39)		probably null	Het
Mtmr14	C	T	6: 113,257,649 (GRCm39)	R233*	probably null	Het
Nbea	T	C	3: 55,937,328 (GRCm39)	Y761C	probably damaging	Het
Neb	A	T	2: 52,067,571 (GRCm39)		probably null	Het
Nup85	T	G	11: 115,455,357 (GRCm39)	M1R	probably null	Het
Obi1	T	C	14: 104,716,289 (GRCm39)	R695G	possibly damaging	Het
Or6c70	T	A	10: 129,709,811 (GRCm39)	I272L	probably benign	Het
Oxr1	T	A	15: 41,680,593 (GRCm39)	M177K	probably damaging	Het
Pgm2l1	A	G	7: 99,901,405 (GRCm39)	Y98C	probably damaging	Het
Pi4kb	G	T	3: 94,904,115 (GRCm39)		probably benign	Het
Pi4kb	G	A	3: 94,904,116 (GRCm39)		probably benign	Het
Pirb	T	A	7: 3,722,247 (GRCm39)	S199C	probably benign	Het
Prss23	A	C	7: 89,159,055 (GRCm39)	I338S	probably damaging	Het
Rapgef3	A	T	15: 97,655,700 (GRCm39)		probably benign	Het
Rdh7	T	A	10: 127,720,539 (GRCm39)	T278S	probably benign	Het
Rrp1b	A	G	17: 32,277,538 (GRCm39)	D606G	probably benign	Het
Rxfp1	T	A	3: 79,559,684 (GRCm39)	Y379F	possibly damaging	Het
Rxfp2	T	C	5: 149,990,853 (GRCm39)	V514A	probably benign	Het
Scel	A	T	14: 103,799,954 (GRCm39)	E202V	probably benign	Het
Slc25a36	G	A	9: 96,962,257 (GRCm39)	A244V	probably benign	Het
Slc2a13	T	G	15: 91,400,595 (GRCm39)	Q209P	probably damaging	Het
Slc38a6	A	G	12: 73,399,304 (GRCm39)	N456S	probably benign	Het
Slc6a12	G	T	6: 121,323,957 (GRCm39)		probably null	Het
Spag6l	T	C	16: 16,598,493 (GRCm39)	I333V	probably benign	Het
Spen	G	A	4: 141,201,514 (GRCm39)	A2371V	probably benign	Het
St6galnac1	T	C	11: 116,657,466 (GRCm39)	D366G	probably damaging	Het
Stk33	T	C	7: 108,940,696 (GRCm39)	S5G	probably benign	Het
Tex56	T	C	13: 35,116,636 (GRCm39)		probably benign	Het
Tle2	T	C	10: 81,413,482 (GRCm39)	L84P	probably damaging	Het
Tmem14a	T	C	1: 21,296,876 (GRCm39)	M78T	probably damaging	Het
Top2b	T	A	14: 16,413,556 (GRCm38)		probably null	Het
Trmt13	A	G	3: 116,376,299 (GRCm39)	F364S	probably damaging	Het
Unkl	T	A	17: 25,449,751 (GRCm39)		probably null	Het
Uvrag	A	G	7: 98,653,926 (GRCm39)		probably benign	Het
Vmn2r8	T	A	5: 108,949,938 (GRCm39)	N303I	probably benign	Het
Zdhhc25	T	C	15: 88,485,123 (GRCm39)	Y153H	probably damaging	Het
Zfp646	T	C	7: 127,482,434 (GRCm39)	V1537A	possibly damaging	Het
Zfp664	T	A	5: 124,963,129 (GRCm39)	Y174*	probably null	Het

Other mutations in Vsig10l

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01319:Vsig10l	APN	7	43,114,678 (GRCm39)	missense	probably damaging	1.00
IGL01390:Vsig10l	APN	7	43,115,889 (GRCm39)	missense	probably damaging	0.97
IGL02401:Vsig10l	APN	7	43,113,468 (GRCm39)	missense	probably damaging	1.00
IGL02404:Vsig10l	APN	7	43,113,171 (GRCm39)	missense	possibly damaging	0.85
IGL02514:Vsig10l	APN	7	43,113,338 (GRCm39)	missense	probably benign	0.02
IGL02636:Vsig10l	APN	7	43,113,002 (GRCm39)	missense	possibly damaging	0.53
IGL02805:Vsig10l	APN	7	43,114,666 (GRCm39)	missense	probably damaging	1.00
IGL02823:Vsig10l	APN	7	43,115,888 (GRCm39)	missense	probably damaging	1.00
IGL02827:Vsig10l	APN	7	43,114,293 (GRCm39)	missense	probably damaging	1.00
R0111:Vsig10l	UTSW	7	43,117,525 (GRCm39)	missense	probably damaging	0.97
R0465:Vsig10l	UTSW	7	43,116,866 (GRCm39)	missense	probably damaging	1.00
R0632:Vsig10l	UTSW	7	43,113,561 (GRCm39)	missense	probably damaging	0.97
R1689:Vsig10l	UTSW	7	43,114,792 (GRCm39)	missense	possibly damaging	0.93
R1991:Vsig10l	UTSW	7	43,116,892 (GRCm39)	missense	possibly damaging	0.89
R2103:Vsig10l	UTSW	7	43,116,892 (GRCm39)	missense	possibly damaging	0.89
R2358:Vsig10l	UTSW	7	43,118,185 (GRCm39)	missense	probably benign	0.00
R3015:Vsig10l	UTSW	7	43,116,881 (GRCm39)	missense	possibly damaging	0.73
R4024:Vsig10l	UTSW	7	43,117,510 (GRCm39)	missense	probably benign	0.33
R4031:Vsig10l	UTSW	7	43,114,798 (GRCm39)	missense	probably damaging	1.00
R4807:Vsig10l	UTSW	7	43,113,173 (GRCm39)	missense	possibly damaging	0.53
R5020:Vsig10l	UTSW	7	43,114,741 (GRCm39)	nonsense	probably null
R5261:Vsig10l	UTSW	7	43,120,274 (GRCm39)	missense	probably damaging	1.00
R5317:Vsig10l	UTSW	7	43,114,247 (GRCm39)	missense	probably damaging	1.00
R5426:Vsig10l	UTSW	7	43,114,247 (GRCm39)	missense	probably damaging	0.97
R5656:Vsig10l	UTSW	7	43,113,575 (GRCm39)	nonsense	probably null
R5842:Vsig10l	UTSW	7	43,118,396 (GRCm39)	missense	probably benign	0.15
R6012:Vsig10l	UTSW	7	43,117,439 (GRCm39)	missense	probably damaging	1.00
R6235:Vsig10l	UTSW	7	43,118,396 (GRCm39)	missense	probably benign	0.15
R6309:Vsig10l	UTSW	7	43,120,397 (GRCm39)	splice site	probably null
R6994:Vsig10l	UTSW	7	43,114,491 (GRCm39)	missense	possibly damaging	0.71
R7250:Vsig10l	UTSW	7	43,113,099 (GRCm39)	missense	probably benign
R7397:Vsig10l	UTSW	7	43,117,431 (GRCm39)	missense	probably damaging	1.00
R7767:Vsig10l	UTSW	7	43,113,141 (GRCm39)	missense	probably damaging	0.96
R7956:Vsig10l	UTSW	7	43,117,494 (GRCm39)	missense	probably benign
R8086:Vsig10l	UTSW	7	43,114,876 (GRCm39)	missense	possibly damaging	0.92
R8139:Vsig10l	UTSW	7	43,113,153 (GRCm39)	missense	probably benign	0.00
R8297:Vsig10l	UTSW	7	43,113,531 (GRCm39)	missense	possibly damaging	0.50
R8341:Vsig10l	UTSW	7	43,113,378 (GRCm39)	missense	probably damaging	1.00
R8519:Vsig10l	UTSW	7	43,114,326 (GRCm39)	missense	probably benign	0.33
R8925:Vsig10l	UTSW	7	43,116,020 (GRCm39)	missense	probably benign	0.41
R8927:Vsig10l	UTSW	7	43,116,020 (GRCm39)	missense	probably benign	0.41
R8948:Vsig10l	UTSW	7	43,117,623 (GRCm39)	missense	possibly damaging	0.53
R9157:Vsig10l	UTSW	7	43,112,948 (GRCm39)	missense
R9203:Vsig10l	UTSW	7	43,112,657 (GRCm39)	start codon destroyed	probably null
R9481:Vsig10l	UTSW	7	43,112,795 (GRCm39)	nonsense	probably null
X0028:Vsig10l	UTSW	7	43,112,788 (GRCm39)	utr 3 prime	probably benign
X0067:Vsig10l	UTSW	7	43,116,914 (GRCm39)	missense	probably damaging	1.00

Predicted Primers

PCR Primer
(F):5'- GGAGAAGTGAGCCCAATGTCTGTG -3'
(R):5'- TGTCCCACCTCACCTATGAAGGTC -3'

Sequencing Primer
(F):5'- GTGAGTTCTTTAACAGTCCAACC -3'
(R):5'- GAGGTTTTCTCACAAACTGCTTG -3'

Posted On

2013-04-24