Mutagenetix > Incidental Mutation

Incidental Mutation 'R3858:Vmn2r86'

276254

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r86

Ensembl Gene

ENSMUSG00000092162

Gene Name

vomeronasal 2, receptor 86

Synonyms

EG625109

MMRRC Submission

040786-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.068) question?

Stock #

R3858 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

130282068-130291763 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

A to G at 130291594 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Methionine to Threonine at position 57 (M57T)

Ref Sequence

ENSEMBL: ENSMUSP00000126596 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000170257]

AlphaFold

G5E8Y4

Predicted Effect

probably benign
Transcript: ENSMUST00000170257
AA Change: M57T

PolyPhen 2 Score 0.000 (Sensitivity: 1.00; Specificity: 0.00)

SMART Domains

Protein: ENSMUSP00000126596
Gene: ENSMUSG00000092162
AA Change: M57T

Domain	Start	End	E-Value	Type
signal peptide	1	24	N/A	INTRINSIC
Pfam:ANF_receptor	77	425	1.1e-25	PFAM
Pfam:NCD3G	508	562	2.4e-19	PFAM
Pfam:7tm_3	595	829	6.4e-55	PFAM

Meta Mutation Damage Score

0.0898

Coding Region Coverage

1x: 99.2%
3x: 98.7%
10x: 97.7%
20x: 96.1%

Validation Efficiency

97% (36/37)

Allele List at MGI

Other mutations in this stock

Total: 32 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Abcb1b	T	A	5: 8,863,581 (GRCm39)	S179T	probably benign	Het
Ahnak	A	G	19: 8,988,223 (GRCm39)	E3169G	possibly damaging	Het
Ccdc82	G	A	9: 13,251,704 (GRCm39)		probably benign	Het
Ccng1	A	G	11: 40,644,660 (GRCm39)	L79P	probably damaging	Het
Cd2ap	G	A	17: 43,127,463 (GRCm39)	Q377*	probably null	Het
Celf1	A	G	2: 90,843,086 (GRCm39)	E411G	probably damaging	Het
Cemip2	A	G	19: 21,829,598 (GRCm39)	T1236A	probably benign	Het
Cps1	A	G	1: 67,207,437 (GRCm39)	Y582C	probably damaging	Het
Efhb	A	G	17: 53,769,808 (GRCm39)	L167S	possibly damaging	Het
Erc2	A	G	14: 28,197,599 (GRCm39)		probably benign	Het
Hs3st1	G	A	5: 39,772,256 (GRCm39)	T129I	probably damaging	Het
Irs4	T	C	X: 140,507,059 (GRCm39)	E379G	probably damaging	Het
Kcnmb2	T	C	3: 32,252,450 (GRCm39)	V217A	probably damaging	Het
Megf10	T	C	18: 57,408,907 (GRCm39)		probably benign	Het
Mib1	T	A	18: 10,798,409 (GRCm39)	C757S	possibly damaging	Het
Mtmr4	G	A	11: 87,488,088 (GRCm39)	V24M	probably damaging	Het
Obscn	A	G	11: 58,971,795 (GRCm39)		probably benign	Het
Or12d13	A	T	17: 37,648,117 (GRCm39)	L2*	probably null	Het
Or5b119	T	A	19: 13,457,494 (GRCm39)	I23F	possibly damaging	Het
Pirb	T	C	7: 3,720,662 (GRCm39)	K279E	possibly damaging	Het
Pmp22	T	C	11: 63,025,301 (GRCm39)	S45P	probably benign	Het
Pth2r	A	T	1: 65,361,206 (GRCm39)	I52F	probably damaging	Het
Reck	A	G	4: 43,930,261 (GRCm39)	T612A	probably benign	Het
Rtn4rl2	A	G	2: 84,710,730 (GRCm39)		probably null	Het
Sis	A	G	3: 72,835,985 (GRCm39)	I868T	probably damaging	Het
Slc23a3	A	G	1: 75,106,040 (GRCm39)		probably null	Het
Slc4a1	A	T	11: 102,247,947 (GRCm39)	V349E	probably benign	Het
Thsd7a	C	T	6: 12,555,225 (GRCm39)	G220S	probably benign	Het
Tle4	G	A	19: 14,445,577 (GRCm39)	T223I	probably benign	Het
Tor3a	T	A	1: 156,497,124 (GRCm39)	L140F	probably damaging	Het
Vcf2	T	C	X: 149,203,357 (GRCm39)	Q39R	probably benign	Het
Zfp512	G	T	5: 31,630,184 (GRCm39)	R222L	probably damaging	Het

Other mutations in Vmn2r86

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL01295:Vmn2r86	APN	10	130,288,895 (GRCm39)	missense	probably damaging	0.99
IGL01328:Vmn2r86	APN	10	130,288,365 (GRCm39)	missense	possibly damaging	0.78
IGL01377:Vmn2r86	APN	10	130,288,855 (GRCm39)	missense	probably damaging	0.99
IGL01548:Vmn2r86	APN	10	130,282,151 (GRCm39)	missense	probably benign	0.22
IGL01804:Vmn2r86	APN	10	130,288,858 (GRCm39)	missense	probably damaging	0.99
IGL01921:Vmn2r86	APN	10	130,291,610 (GRCm39)	missense	probably benign	0.00
IGL02406:Vmn2r86	APN	10	130,284,508 (GRCm39)	missense	possibly damaging	0.81
IGL02625:Vmn2r86	APN	10	130,288,781 (GRCm39)	missense	probably damaging	1.00
IGL02960:Vmn2r86	APN	10	130,289,636 (GRCm39)	missense	possibly damaging	0.74
IGL03104:Vmn2r86	APN	10	130,282,501 (GRCm39)	missense	probably damaging	1.00
R0408:Vmn2r86	UTSW	10	130,282,723 (GRCm39)	missense	probably damaging	1.00
R0437:Vmn2r86	UTSW	10	130,282,412 (GRCm39)	missense	probably damaging	1.00
R0577:Vmn2r86	UTSW	10	130,288,444 (GRCm39)	missense	probably benign	0.04
R0726:Vmn2r86	UTSW	10	130,282,265 (GRCm39)	missense	probably damaging	1.00
R0811:Vmn2r86	UTSW	10	130,289,497 (GRCm39)	missense	probably benign	0.00
R0812:Vmn2r86	UTSW	10	130,289,497 (GRCm39)	missense	probably benign	0.00
R1055:Vmn2r86	UTSW	10	130,282,226 (GRCm39)	missense	probably damaging	1.00
R1066:Vmn2r86	UTSW	10	130,282,145 (GRCm39)	missense	probably benign	0.01
R1199:Vmn2r86	UTSW	10	130,284,443 (GRCm39)	splice site	probably benign
R1332:Vmn2r86	UTSW	10	130,282,739 (GRCm39)	missense	probably damaging	1.00
R1568:Vmn2r86	UTSW	10	130,289,010 (GRCm39)	missense	probably benign	0.09
R1866:Vmn2r86	UTSW	10	130,282,255 (GRCm39)	missense	probably damaging	1.00
R1897:Vmn2r86	UTSW	10	130,288,314 (GRCm39)	missense	probably damaging	1.00
R2017:Vmn2r86	UTSW	10	130,282,582 (GRCm39)	missense	probably benign	0.39
R3162:Vmn2r86	UTSW	10	130,291,673 (GRCm39)	missense	probably damaging	0.99
R3162:Vmn2r86	UTSW	10	130,291,673 (GRCm39)	missense	probably damaging	0.99
R4049:Vmn2r86	UTSW	10	130,282,966 (GRCm39)	missense	probably damaging	0.98
R4378:Vmn2r86	UTSW	10	130,288,469 (GRCm39)	missense	possibly damaging	0.67
R4411:Vmn2r86	UTSW	10	130,288,469 (GRCm39)	missense	possibly damaging	0.67
R4413:Vmn2r86	UTSW	10	130,288,469 (GRCm39)	missense	possibly damaging	0.67
R4422:Vmn2r86	UTSW	10	130,288,845 (GRCm39)	missense	possibly damaging	0.87
R4738:Vmn2r86	UTSW	10	130,282,939 (GRCm39)	missense	probably damaging	0.99
R4767:Vmn2r86	UTSW	10	130,291,606 (GRCm39)	missense	probably benign	0.00
R4872:Vmn2r86	UTSW	10	130,289,460 (GRCm39)	missense	probably damaging	0.98
R4880:Vmn2r86	UTSW	10	130,289,484 (GRCm39)	missense	probably benign	0.33
R5092:Vmn2r86	UTSW	10	130,282,456 (GRCm39)	missense	probably damaging	1.00
R5421:Vmn2r86	UTSW	10	130,282,805 (GRCm39)	missense	probably benign	0.41
R6007:Vmn2r86	UTSW	10	130,289,535 (GRCm39)	missense	probably damaging	1.00
R6330:Vmn2r86	UTSW	10	130,282,396 (GRCm39)	missense	probably benign	0.05
R6355:Vmn2r86	UTSW	10	130,291,763 (GRCm39)	start codon destroyed	probably damaging	0.98
R6397:Vmn2r86	UTSW	10	130,282,131 (GRCm39)	nonsense	probably null
R6419:Vmn2r86	UTSW	10	130,282,795 (GRCm39)	missense	probably damaging	1.00
R6933:Vmn2r86	UTSW	10	130,282,126 (GRCm39)	missense	probably damaging	1.00
R6937:Vmn2r86	UTSW	10	130,284,523 (GRCm39)	missense	probably damaging	1.00
R6959:Vmn2r86	UTSW	10	130,282,400 (GRCm39)	missense	probably damaging	1.00
R7010:Vmn2r86	UTSW	10	130,291,726 (GRCm39)	missense	probably benign
R7549:Vmn2r86	UTSW	10	130,282,697 (GRCm39)	missense	probably damaging	1.00
R8179:Vmn2r86	UTSW	10	130,288,953 (GRCm39)	missense	probably benign	0.00
R8257:Vmn2r86	UTSW	10	130,288,279 (GRCm39)	missense	possibly damaging	0.87
R8286:Vmn2r86	UTSW	10	130,285,855 (GRCm39)	missense	probably benign	0.03
R8479:Vmn2r86	UTSW	10	130,282,735 (GRCm39)	missense	probably damaging	1.00
R8805:Vmn2r86	UTSW	10	130,282,396 (GRCm39)	missense	probably benign	0.05
R8960:Vmn2r86	UTSW	10	130,289,672 (GRCm39)	missense	probably benign	0.27
R9021:Vmn2r86	UTSW	10	130,282,934 (GRCm39)	missense	probably damaging	1.00
R9120:Vmn2r86	UTSW	10	130,289,677 (GRCm39)	missense	probably benign	0.00
R9137:Vmn2r86	UTSW	10	130,282,409 (GRCm39)	missense	probably damaging	1.00
R9311:Vmn2r86	UTSW	10	130,288,440 (GRCm39)	missense	probably damaging	1.00
R9312:Vmn2r86	UTSW	10	130,288,406 (GRCm39)	missense	probably benign	0.02
R9433:Vmn2r86	UTSW	10	130,282,567 (GRCm39)	missense	possibly damaging	0.88
R9696:Vmn2r86	UTSW	10	130,285,702 (GRCm39)	missense	possibly damaging	0.49

Predicted Primers

PCR Primer
(F):5'- CCAGCATTGTGAACTGGAGAAC -3'
(R):5'- CCATCTTAGATTCTAGCACACATGAAG -3'

Sequencing Primer
(F):5'- CTAAAGTTACAGGGGGTGA -3'
(R):5'- GATTCTAGCACACATGAAGAAAATG -3'

Posted On

2015-04-06