Mutagenetix > Incidental Mutation

Incidental Mutation 'R2229:Myo7a'

239601

Institutional Source

Beutler Lab

Gene Symbol

Myo7a

Ensembl Gene

ENSMUSG00000030761

Gene Name

myosin VIIA

Synonyms

nmf371, USH1B, polka, Hdb, Myo7

MMRRC Submission

040230-MU

Accession Numbers

Essential gene?

Non essential (E-score: 0.000) question?

Stock #

R2229 (G1)

Quality Score

225

Status

Validated

Chromosome

Chromosomal Location

97700267-97768731 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to C at 97704117 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Threonine to Alanine at position 1932 (T1932A)

Ref Sequence

ENSEMBL: ENSMUSP00000102744 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000084979] [ENSMUST00000107122] [ENSMUST00000107127] [ENSMUST00000107128] [ENSMUST00000156992] [ENSMUST00000205746]

AlphaFold

P97479

PDB Structure

Structure of myosin VIIa MyTH4-FERM-SH3 in complex with the CEN1 of Sans [X-RAY DIFFRACTION]

Predicted Effect

probably benign
Transcript: ENSMUST00000084979
AA Change: T1921A

PolyPhen 2 Score 0.014 (Sensitivity: 0.96; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000082046
Gene: ENSMUSG00000030761
AA Change: T1921A

Domain	Start	End	E-Value	Type
MYSc	48	731	N/A	SMART
IQ	732	754	2.99e0	SMART
IQ	755	777	8.77e-7	SMART
IQ	801	823	8e0	SMART
IQ	824	846	8.7e0	SMART
low complexity region	854	889	N/A	INTRINSIC
low complexity region	893	916	N/A	INTRINSIC
low complexity region	972	985	N/A	INTRINSIC
MyTH4	1006	1242	1.4e-71	SMART
B41	1243	1458	8.82e-42	SMART
SH3	1557	1622	4.93e-7	SMART
MyTH4	1698	1847	3.95e-57	SMART
B41	1849	2066	8.27e-56	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107122
AA Change: T1927A

PolyPhen 2 Score 0.016 (Sensitivity: 0.95; Specificity: 0.79)

SMART Domains

Protein: ENSMUSP00000102739
Gene: ENSMUSG00000030761
AA Change: T1927A

Domain	Start	End	E-Value	Type
MYSc	48	737	N/A	SMART
IQ	738	760	2.99e0	SMART
IQ	761	783	8.77e-7	SMART
IQ	807	829	8e0	SMART
IQ	830	852	8.7e0	SMART
low complexity region	860	895	N/A	INTRINSIC
low complexity region	899	922	N/A	INTRINSIC
low complexity region	978	991	N/A	INTRINSIC
MyTH4	1012	1248	1.4e-71	SMART
B41	1249	1464	8.82e-42	SMART
SH3	1563	1628	4.93e-7	SMART
MyTH4	1704	1853	3.95e-57	SMART
B41	1855	2072	8.27e-56	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107127
AA Change: T1932A

PolyPhen 2 Score 0.119 (Sensitivity: 0.93; Specificity: 0.86)

SMART Domains

Protein: ENSMUSP00000102744
Gene: ENSMUSG00000030761
AA Change: T1932A

Domain	Start	End	E-Value	Type
MYSc	59	742	N/A	SMART
IQ	743	765	2.99e0	SMART
IQ	766	788	8.77e-7	SMART
IQ	812	834	8e0	SMART
IQ	835	857	8.7e0	SMART
low complexity region	865	900	N/A	INTRINSIC
low complexity region	904	927	N/A	INTRINSIC
low complexity region	983	996	N/A	INTRINSIC
MyTH4	1017	1253	1.4e-71	SMART
B41	1254	1469	8.82e-42	SMART
SH3	1568	1633	4.93e-7	SMART
MyTH4	1709	1858	3.95e-57	SMART
B41	1860	2077	8.27e-56	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000107128
AA Change: T1970A

PolyPhen 2 Score 0.025 (Sensitivity: 0.95; Specificity: 0.81)

SMART Domains

Protein: ENSMUSP00000102745
Gene: ENSMUSG00000030761
AA Change: T1970A

Domain	Start	End	E-Value	Type
MYSc	59	742	N/A	SMART
IQ	743	765	2.99e0	SMART
IQ	766	788	8.77e-7	SMART
IQ	812	834	8e0	SMART
IQ	835	857	8.7e0	SMART
low complexity region	865	900	N/A	INTRINSIC
low complexity region	904	927	N/A	INTRINSIC
low complexity region	983	996	N/A	INTRINSIC
MyTH4	1017	1253	1.4e-71	SMART
B41	1254	1469	8.82e-42	SMART
SH3	1606	1671	4.93e-7	SMART
MyTH4	1747	1896	3.95e-57	SMART
B41	1898	2115	8.27e-56	SMART

Predicted Effect

probably benign
Transcript: ENSMUST00000156992

Predicted Effect

probably benign
Transcript: ENSMUST00000205746
AA Change: T1921A

PolyPhen 2 Score 0.010 (Sensitivity: 0.96; Specificity: 0.77)

Meta Mutation Damage Score

0.1064

Coding Region Coverage

1x: 99.2%
3x: 98.6%
10x: 97.3%
20x: 95.1%

Validation Efficiency

99% (78/79)

MGI Phenotype

FUNCTION: [Summary is not available for the mouse gene. This summary is for the human ortholog.] This gene is a member of the myosin gene family. Myosins are mechanochemical proteins characterized by the presence of a motor domain, an actin-binding domain, a neck domain that interacts with other proteins, and a tail domain that serves as an anchor. This gene encodes an unconventional myosin with a very short tail. Defects in this gene are associated with the mouse shaker-1 phenotype and the human Usher syndrome 1B which are characterized by deafness, reduced vestibular function, and (in human) retinal degeneration. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
PHENOTYPE: A number of spontaneous and ENU-induced mutations cause head-shaking, circling and deafness, often associated with cochlear hair cell degeneration and stereocilia anomalies. Defects in retinal pigment epithelial cells, male infertility, and light-inducedphotoreceptor damage have also been observed. [provided by MGI curators]

Allele List at MGI

Other mutations in this stock

Total: 74 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Adam24	A	T	8: 41,133,404 (GRCm39)	I291L	probably benign	Het
Adcy8	C	A	15: 64,694,056 (GRCm39)	R407L	possibly damaging	Het
Adgb	A	T	10: 10,311,795 (GRCm39)	V212E	probably damaging	Het
Adgrg7	C	T	16: 56,572,766 (GRCm39)	S350N	probably benign	Het
Agbl1	A	G	7: 76,083,126 (GRCm39)	T448A	probably benign	Het
Aldh1l1	C	G	6: 90,560,168 (GRCm39)	T605R	probably damaging	Het
Arfip1	A	T	3: 84,455,280 (GRCm39)	N18K	probably damaging	Het
Atp6v1b2	C	A	8: 69,555,411 (GRCm39)		probably null	Het
Banp	G	A	8: 122,705,424 (GRCm39)	S98N	probably damaging	Het
Btnl9	T	C	11: 49,059,945 (GRCm39)	D601G	probably damaging	Het
C9	T	C	15: 6,474,901 (GRCm39)	I20T	possibly damaging	Het
Cacna1b	A	G	2: 24,575,816 (GRCm39)	V744A	probably damaging	Het
Catsper3	A	G	13: 55,955,867 (GRCm39)	E311G	probably damaging	Het
Ccdc180	G	T	4: 45,948,856 (GRCm39)		probably null	Het
Cdc5l	A	G	17: 45,718,772 (GRCm39)	Y615H	probably benign	Het
Crybg2	TGGAGGAGGAGGAGGAGGAG	TGGAGGAGGAGGAGGAG	4: 133,801,837 (GRCm39)		probably benign	Het
Eml4	C	T	17: 83,758,485 (GRCm39)	P502S	probably benign	Het
Fsip1	T	C	2: 118,052,925 (GRCm39)	E367G	probably benign	Het
Gja3	T	C	14: 57,274,171 (GRCm39)	D67G	probably damaging	Het
Gm4894	T	C	9: 49,185,490 (GRCm39)		probably benign	Het
Gm7853	A	G	14: 35,811,484 (GRCm39)		noncoding transcript	Het
Gmps	T	G	3: 63,921,684 (GRCm39)	Y562*	probably null	Het
Golga2	C	A	2: 32,196,477 (GRCm39)	P976T	probably benign	Het
Gpr182	T	A	10: 127,586,010 (GRCm39)	I314F	possibly damaging	Het
Gprc6a	A	G	10: 51,502,891 (GRCm39)	V324A	possibly damaging	Het
Gykl1	A	G	18: 52,828,339 (GRCm39)	T516A	probably benign	Het
H2ac21	T	C	3: 96,127,422 (GRCm39)	L64P	possibly damaging	Het
Ifit1bl2	G	T	19: 34,596,630 (GRCm39)	L329M	possibly damaging	Het
Igsf9b	T	C	9: 27,244,792 (GRCm39)	S920P	probably damaging	Het
Kif3c	T	A	12: 3,416,671 (GRCm39)	S231T	probably benign	Het
Kpna7	A	T	5: 144,926,507 (GRCm39)	Y482N	probably damaging	Het
Lmnb2	T	C	10: 80,740,226 (GRCm39)		probably benign	Het
Lrrc69	T	C	4: 14,773,694 (GRCm39)	S121G	probably benign	Het
Mppe1	A	G	18: 67,361,082 (GRCm39)		probably null	Het
Muc5b	T	A	7: 141,415,381 (GRCm39)	C2776S	possibly damaging	Het
Myh8	T	G	11: 67,199,174 (GRCm39)	N1893K	probably damaging	Het
Nbn	A	G	4: 15,970,904 (GRCm39)	T296A	probably benign	Het
Nckap1l	T	C	15: 103,364,361 (GRCm39)		probably null	Het
Nek5	A	T	8: 22,603,648 (GRCm39)	N151K	possibly damaging	Het
Nup93	C	T	8: 95,030,819 (GRCm39)	T305I	probably benign	Het
Oca2	T	A	7: 56,006,903 (GRCm39)	H663Q	probably benign	Het
Optn	T	A	2: 5,028,928 (GRCm39)	H525L	probably damaging	Het
Pdzph1	A	G	17: 59,239,407 (GRCm39)		probably benign	Het
Pikfyve	A	G	1: 65,307,014 (GRCm39)	K1801E	probably damaging	Het
Pkd2l2	G	A	18: 34,563,382 (GRCm39)	V478M	probably damaging	Het
Pmepa1	G	A	2: 173,069,926 (GRCm39)	R210W	probably damaging	Het
Ppp1r3c	C	A	19: 36,711,098 (GRCm39)	R224L	probably benign	Het
Prpf19	A	G	19: 10,874,962 (GRCm39)	T39A	probably benign	Het
Pwwp2b	T	C	7: 138,835,104 (GRCm39)	C182R	probably damaging	Het
Rcan3	T	C	4: 135,152,688 (GRCm39)	D11G	probably benign	Het
Rgsl1	C	A	1: 153,698,104 (GRCm39)	W482L	possibly damaging	Het
Sfxn4	C	T	19: 60,839,458 (GRCm39)	G200E	probably damaging	Het
Slc4a8	T	C	15: 100,707,180 (GRCm39)	I848T	probably damaging	Het
Slc7a13	T	C	4: 19,839,399 (GRCm39)	V334A	probably benign	Het
Smarcc2	A	G	10: 128,324,210 (GRCm39)		probably benign	Het
Spata18	A	T	5: 73,824,244 (GRCm39)	I156L	possibly damaging	Het
Spats2	T	C	15: 99,072,334 (GRCm39)		probably null	Het
Tas2r104	G	A	6: 131,662,095 (GRCm39)	H205Y	probably damaging	Het
Tcof1	A	G	18: 60,965,249 (GRCm39)		probably benign	Het
Tex15	C	A	8: 34,061,265 (GRCm39)	H232N	probably benign	Het
Tg	A	T	15: 66,545,860 (GRCm39)	Q194L	probably damaging	Het
Tnfrsf22	C	T	7: 143,198,513 (GRCm39)		probably null	Het
Trim25	T	A	11: 88,907,447 (GRCm39)	V602E	probably damaging	Het
Ttll9	A	G	2: 152,824,983 (GRCm39)	E54G	probably damaging	Het
Ttn	T	C	2: 76,559,668 (GRCm39)	T29578A	probably damaging	Het
Tubgcp5	A	G	7: 55,480,629 (GRCm39)	Q960R	probably damaging	Het
Ugcg	C	T	4: 59,207,798 (GRCm39)	P46S	probably benign	Het
Usp13	A	G	3: 32,971,700 (GRCm39)	I727V	probably benign	Het
Vmn2r67	T	A	7: 84,801,250 (GRCm39)	I229F	probably benign	Het
Vmn2r92	A	C	17: 18,387,654 (GRCm39)	I220L	probably benign	Het
Wnk4	T	A	11: 101,166,467 (GRCm39)		probably benign	Het
Wwp1	A	T	4: 19,641,745 (GRCm39)	Y437N	probably damaging	Het
Zdhhc5	A	G	2: 84,520,557 (GRCm39)	I540T	probably damaging	Het
Zfp729b	T	C	13: 67,743,384 (GRCm39)	I60M	probably damaging	Het

Other mutations in Myo7a

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00465:Myo7a	APN	7	97,751,833 (GRCm39)	missense	probably damaging	1.00
IGL00785:Myo7a	APN	7	97,703,555 (GRCm39)	missense	probably damaging	0.99
IGL00840:Myo7a	APN	7	97,700,866 (GRCm39)	missense	probably benign	0.25
IGL01362:Myo7a	APN	7	97,746,909 (GRCm39)	missense	probably damaging	1.00
IGL01484:Myo7a	APN	7	97,734,629 (GRCm39)	missense	probably damaging	1.00
IGL01673:Myo7a	APN	7	97,703,915 (GRCm39)	missense	probably benign	0.00
IGL01933:Myo7a	APN	7	97,732,349 (GRCm39)	missense	probably damaging	1.00
IGL01943:Myo7a	APN	7	97,714,854 (GRCm39)	missense	possibly damaging	0.96
IGL02188:Myo7a	APN	7	97,740,234 (GRCm39)	missense	probably damaging	0.96
IGL02304:Myo7a	APN	7	97,726,943 (GRCm39)	missense	possibly damaging	0.89
IGL02305:Myo7a	APN	7	97,700,836 (GRCm39)	makesense	probably null
IGL02331:Myo7a	APN	7	97,702,389 (GRCm39)	missense	possibly damaging	0.95
IGL02386:Myo7a	APN	7	97,724,319 (GRCm39)	missense	probably damaging	0.99
IGL02389:Myo7a	APN	7	97,756,198 (GRCm39)	critical splice donor site	probably null
IGL02832:Myo7a	APN	7	97,740,227 (GRCm39)	critical splice donor site	probably null
IGL02839:Myo7a	APN	7	97,740,329 (GRCm39)	missense	probably damaging	1.00
IGL03193:Myo7a	APN	7	97,740,264 (GRCm39)	missense	probably damaging	1.00
IGL03237:Myo7a	APN	7	97,751,800 (GRCm39)	missense	probably damaging	1.00
IGL03384:Myo7a	APN	7	97,742,800 (GRCm39)	missense	probably damaging	1.00
coward	UTSW	7	97,734,673 (GRCm39)	missense	probably damaging	1.00
H8786:Myo7a	UTSW	7	97,744,985 (GRCm39)	missense	possibly damaging	0.61
IGL03046:Myo7a	UTSW	7	97,728,534 (GRCm39)	missense	probably damaging	1.00
IGL03134:Myo7a	UTSW	7	97,705,974 (GRCm39)	missense	probably damaging	0.96
PIT4696001:Myo7a	UTSW	7	97,712,806 (GRCm39)	missense	probably benign	0.00
R0054:Myo7a	UTSW	7	97,714,905 (GRCm39)	missense	probably damaging	1.00
R0054:Myo7a	UTSW	7	97,714,905 (GRCm39)	missense	probably damaging	1.00
R0071:Myo7a	UTSW	7	97,706,037 (GRCm39)	missense	probably damaging	0.98
R0071:Myo7a	UTSW	7	97,706,037 (GRCm39)	missense	probably damaging	0.98
R0267:Myo7a	UTSW	7	97,703,831 (GRCm39)	missense	probably benign	0.08
R0408:Myo7a	UTSW	7	97,705,988 (GRCm39)	missense	probably damaging	1.00
R0411:Myo7a	UTSW	7	97,721,144 (GRCm39)	missense	probably benign	0.00
R0540:Myo7a	UTSW	7	97,721,153 (GRCm39)	missense	probably damaging	1.00
R0607:Myo7a	UTSW	7	97,721,153 (GRCm39)	missense	probably damaging	1.00
R0629:Myo7a	UTSW	7	97,734,673 (GRCm39)	missense	probably damaging	1.00
R0632:Myo7a	UTSW	7	97,761,357 (GRCm39)	intron	probably benign
R0659:Myo7a	UTSW	7	97,703,545 (GRCm39)	splice site	probably benign
R0735:Myo7a	UTSW	7	97,730,387 (GRCm39)	splice site	probably benign
R0924:Myo7a	UTSW	7	97,747,463 (GRCm39)	missense	probably damaging	0.99
R0930:Myo7a	UTSW	7	97,747,463 (GRCm39)	missense	probably damaging	0.99
R1018:Myo7a	UTSW	7	97,756,212 (GRCm39)	missense	probably damaging	1.00
R1196:Myo7a	UTSW	7	97,746,880 (GRCm39)	missense	possibly damaging	0.87
R1331:Myo7a	UTSW	7	97,756,215 (GRCm39)	missense	probably benign	0.00
R1487:Myo7a	UTSW	7	97,703,017 (GRCm39)	critical splice donor site	probably null
R1676:Myo7a	UTSW	7	97,748,679 (GRCm39)	critical splice donor site	probably null
R1695:Myo7a	UTSW	7	97,741,703 (GRCm39)	missense	possibly damaging	0.94
R1770:Myo7a	UTSW	7	97,761,813 (GRCm39)	intron	probably benign
R1781:Myo7a	UTSW	7	97,722,331 (GRCm39)	missense	probably damaging	1.00
R1789:Myo7a	UTSW	7	97,756,302 (GRCm39)	missense	probably damaging	0.99
R1827:Myo7a	UTSW	7	97,725,938 (GRCm39)	missense	probably damaging	0.99
R1864:Myo7a	UTSW	7	97,701,463 (GRCm39)	missense	probably damaging	1.00
R1955:Myo7a	UTSW	7	97,704,128 (GRCm39)	missense	probably damaging	1.00
R2011:Myo7a	UTSW	7	97,703,915 (GRCm39)	missense	possibly damaging	0.69
R2259:Myo7a	UTSW	7	97,718,706 (GRCm39)	missense	probably damaging	1.00
R2443:Myo7a	UTSW	7	97,744,976 (GRCm39)	missense	probably benign	0.07
R2898:Myo7a	UTSW	7	97,746,413 (GRCm39)	missense	probably damaging	1.00
R2898:Myo7a	UTSW	7	97,703,631 (GRCm39)	nonsense	probably null
R3158:Myo7a	UTSW	7	97,701,499 (GRCm39)	missense	probably damaging	1.00
R3408:Myo7a	UTSW	7	97,730,294 (GRCm39)	missense	probably benign	0.00
R4222:Myo7a	UTSW	7	97,722,436 (GRCm39)	missense	possibly damaging	0.93
R4255:Myo7a	UTSW	7	97,721,171 (GRCm39)	missense	probably damaging	0.96
R4374:Myo7a	UTSW	7	97,751,881 (GRCm39)	missense	probably damaging	1.00
R4429:Myo7a	UTSW	7	97,702,395 (GRCm39)	missense	probably damaging	0.99
R4445:Myo7a	UTSW	7	97,715,611 (GRCm39)	missense	probably damaging	1.00
R4579:Myo7a	UTSW	7	97,722,400 (GRCm39)	missense	probably damaging	1.00
R4659:Myo7a	UTSW	7	97,734,673 (GRCm39)	missense	probably damaging	1.00
R5073:Myo7a	UTSW	7	97,722,425 (GRCm39)	nonsense	probably null
R5138:Myo7a	UTSW	7	97,732,806 (GRCm39)	missense	probably damaging	1.00
R5566:Myo7a	UTSW	7	97,714,023 (GRCm39)	missense	possibly damaging	0.93
R5580:Myo7a	UTSW	7	97,722,367 (GRCm39)	missense	probably damaging	1.00
R6079:Myo7a	UTSW	7	97,714,997 (GRCm39)	nonsense	probably null
R6138:Myo7a	UTSW	7	97,714,997 (GRCm39)	nonsense	probably null
R6451:Myo7a	UTSW	7	97,722,374 (GRCm39)	missense	probably benign	0.01
R6452:Myo7a	UTSW	7	97,722,374 (GRCm39)	missense	probably benign	0.01
R6453:Myo7a	UTSW	7	97,722,374 (GRCm39)	missense	probably benign	0.01
R6454:Myo7a	UTSW	7	97,722,374 (GRCm39)	missense	probably benign	0.01
R6455:Myo7a	UTSW	7	97,722,374 (GRCm39)	missense	probably benign	0.01
R6465:Myo7a	UTSW	7	97,711,887 (GRCm39)	missense	possibly damaging	0.95
R6653:Myo7a	UTSW	7	97,703,710 (GRCm39)	missense	probably damaging	0.96
R6709:Myo7a	UTSW	7	97,703,906 (GRCm39)	missense	probably damaging	1.00
R6917:Myo7a	UTSW	7	97,744,970 (GRCm39)	missense	possibly damaging	0.58
R7313:Myo7a	UTSW	7	97,713,402 (GRCm39)	missense	probably damaging	0.99
R7334:Myo7a	UTSW	7	97,728,573 (GRCm39)	missense	probably benign
R7356:Myo7a	UTSW	7	97,751,890 (GRCm39)	missense	probably benign	0.01
R7393:Myo7a	UTSW	7	97,712,906 (GRCm39)	missense	possibly damaging	0.91
R7422:Myo7a	UTSW	7	97,700,833 (GRCm39)	splice site	probably null
R7472:Myo7a	UTSW	7	97,714,000 (GRCm39)	missense	probably damaging	1.00
R7483:Myo7a	UTSW	7	97,712,881 (GRCm39)	missense	probably benign	0.07
R7526:Myo7a	UTSW	7	97,734,655 (GRCm39)	missense	possibly damaging	0.49
R7948:Myo7a	UTSW	7	97,724,236 (GRCm39)	missense	probably damaging	1.00
R8069:Myo7a	UTSW	7	97,732,833 (GRCm39)	nonsense	probably null
R8115:Myo7a	UTSW	7	97,715,653 (GRCm39)	missense	probably damaging	0.98
R8150:Myo7a	UTSW	7	97,712,846 (GRCm39)	missense	probably benign	0.19
R8265:Myo7a	UTSW	7	97,734,604 (GRCm39)	missense	probably benign	0.00
R8289:Myo7a	UTSW	7	97,726,376 (GRCm39)	missense	probably benign
R8298:Myo7a	UTSW	7	97,747,541 (GRCm39)	missense	probably damaging	1.00
R8518:Myo7a	UTSW	7	97,740,270 (GRCm39)	missense	possibly damaging	0.58
R8539:Myo7a	UTSW	7	97,721,668 (GRCm39)	missense	probably damaging	0.99
R8557:Myo7a	UTSW	7	97,703,081 (GRCm39)	missense	probably benign	0.08
R8685:Myo7a	UTSW	7	97,746,334 (GRCm39)	missense	probably benign	0.03
R8902:Myo7a	UTSW	7	97,741,820 (GRCm39)	missense	probably damaging	1.00
R9034:Myo7a	UTSW	7	97,728,465 (GRCm39)	missense	probably benign	0.40
R9090:Myo7a	UTSW	7	97,740,281 (GRCm39)	missense	probably benign	0.04
R9172:Myo7a	UTSW	7	97,732,369 (GRCm39)	missense	probably benign
R9271:Myo7a	UTSW	7	97,740,281 (GRCm39)	missense	probably benign	0.04
R9334:Myo7a	UTSW	7	97,716,369 (GRCm39)	missense	probably damaging	1.00
R9356:Myo7a	UTSW	7	97,725,873 (GRCm39)	missense	probably benign	0.11
R9444:Myo7a	UTSW	7	97,742,698 (GRCm39)	missense	possibly damaging	0.84
R9459:Myo7a	UTSW	7	97,722,380 (GRCm39)	missense	possibly damaging	0.65
R9513:Myo7a	UTSW	7	97,746,818 (GRCm39)	critical splice donor site	probably null
R9517:Myo7a	UTSW	7	97,721,166 (GRCm39)	missense	probably damaging	1.00
R9629:Myo7a	UTSW	7	97,712,937 (GRCm39)	missense	probably benign	0.03
R9662:Myo7a	UTSW	7	97,747,499 (GRCm39)	missense	possibly damaging	0.55
R9709:Myo7a	UTSW	7	97,743,536 (GRCm39)	missense	possibly damaging	0.79
RF005:Myo7a	UTSW	7	97,742,824 (GRCm39)	missense	probably benign	0.42
U15987:Myo7a	UTSW	7	97,714,997 (GRCm39)	nonsense	probably null
X0028:Myo7a	UTSW	7	97,714,932 (GRCm39)	missense	probably damaging	1.00
X0058:Myo7a	UTSW	7	97,711,855 (GRCm39)	missense	probably benign	0.02
Z1176:Myo7a	UTSW	7	97,744,934 (GRCm39)	missense	probably damaging	0.98
Z1177:Myo7a	UTSW	7	97,734,730 (GRCm39)	critical splice acceptor site	probably null
Z1177:Myo7a	UTSW	7	97,701,433 (GRCm39)	missense	probably damaging	0.98

Predicted Primers

PCR Primer
(F):5'- TCTTCATGAAGAACACCTGGTAG -3'
(R):5'- ATGCTAACGAGGCACCAGTG -3'

Sequencing Primer
(F):5'- ACGATTCCTGTGGCACAG -3'
(R):5'- TGGTGTGGGTAGGACCAAC -3'

Posted On

2014-10-15