Incidental Mutation 'R0711:Daw1'
ID 218641
Institutional Source Beutler Lab
Gene Symbol Daw1
Ensembl Gene ENSMUSG00000053161
Gene Name dynein assembly factor with WDR repeat domains 1
Synonyms b2b1584Clo, Wdr69, b2b1116Clo, 4933429D11Rik, 4930563E19Rik
MMRRC Submission 038894-MU
Accession Numbers
Essential gene? Probably non essential (E-score: 0.090) question?
Stock # R0711 (G1)
Quality Score 79
Status Validated
Chromosome 1
Chromosomal Location 83137473-83188295 bp(+) (GRCm39)
Type of Mutation splice site
DNA Base Change (assembly) T to C at 83169059 bp (GRCm39)
Zygosity Heterozygous
Amino Acid Change
Ref Sequence ENSEMBL: ENSMUSP00000117796 (fasta)
Gene Model predicted gene model for transcript(s): [ENSMUST00000065403] [ENSMUST00000065436] [ENSMUST00000113436] [ENSMUST00000149342]
AlphaFold D3Z7A5
Predicted Effect probably benign
Transcript: ENSMUST00000065403
SMART Domains Protein: ENSMUSP00000067583
Gene: ENSMUSG00000053161

DomainStartEndE-ValueType
WD40 81 120 1.17e-5 SMART
WD40 123 163 1.14e-8 SMART
WD40 166 205 1.95e-11 SMART
WD40 208 247 3.47e-8 SMART
WD40 250 289 2.98e-7 SMART
WD40 292 331 1.51e-8 SMART
WD40 334 373 4.87e-12 SMART
WD40 376 415 5.14e-11 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000065436
SMART Domains Protein: ENSMUSP00000067102
Gene: ENSMUSG00000053161

DomainStartEndE-ValueType
WD40 81 120 1.17e-5 SMART
WD40 145 184 3.37e-6 SMART
WD40 187 226 1.51e-8 SMART
WD40 229 268 4.87e-12 SMART
WD40 271 310 5.14e-11 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000113436
SMART Domains Protein: ENSMUSP00000109063
Gene: ENSMUSG00000053161

DomainStartEndE-ValueType
WD40 81 120 1.17e-5 SMART
WD40 145 184 3.37e-6 SMART
WD40 187 226 1.51e-8 SMART
WD40 229 268 4.87e-12 SMART
WD40 271 317 7.99e-1 SMART
Predicted Effect probably benign
Transcript: ENSMUST00000149342
SMART Domains Protein: ENSMUSP00000117796
Gene: ENSMUSG00000053161

DomainStartEndE-ValueType
low complexity region 36 48 N/A INTRINSIC
Meta Mutation Damage Score 0.0898 question?
Coding Region Coverage
  • 1x: 99.1%
  • 3x: 98.4%
  • 10x: 96.4%
  • 20x: 91.4%
Validation Efficiency 100% (91/91)
MGI Phenotype PHENOTYPE: Mice homozygous for an induced mutation exhibit dextrocardia associated with situs inversus totalis, overriding aorta, ventricular septal defects, and dual inferior vena cava as well as dextrogastria, hypoplastic spleen, inverted liver, lung lobation/isomerism and dyskinetic/immotile airway cilia [provided by MGI curators]
Allele List at MGI
Other mutations in this stock
Total: 68 list
GeneRefVarChr/LocMutationPredicted EffectZygosity
1110002E22Rik T A 3: 137,773,986 (GRCm39) D1058E probably damaging Het
4933405L10Rik A T 8: 106,435,563 (GRCm39) probably null Het
Adamtsl3 T A 7: 82,114,907 (GRCm39) probably benign Het
Afdn C T 17: 14,072,698 (GRCm39) P874S probably damaging Het
Ankrd6 G A 4: 32,815,326 (GRCm39) A391V probably damaging Het
Arhgef28 T G 13: 98,067,762 (GRCm39) T1388P probably damaging Het
Asxl3 G T 18: 22,657,508 (GRCm39) M1839I probably benign Het
BC005537 T C 13: 24,989,923 (GRCm39) F129L probably damaging Het
Celf2 A G 2: 6,726,226 (GRCm39) probably null Het
Chid1 C T 7: 141,076,590 (GRCm39) V325I probably benign Het
Cnn3 T A 3: 121,243,633 (GRCm39) D31E probably benign Het
Col12a1 G A 9: 79,559,317 (GRCm39) P1857L probably damaging Het
Cpeb1 T A 7: 81,001,618 (GRCm39) R430W probably benign Het
Dcaf13 A G 15: 39,001,484 (GRCm39) Y264C probably damaging Het
Dnah6 T C 6: 73,064,585 (GRCm39) I2666V probably damaging Het
Dnai2 A C 11: 114,645,158 (GRCm39) D531A probably benign Het
Dock10 A T 1: 80,501,692 (GRCm39) F1833I probably damaging Het
Efhd2 C T 4: 141,587,183 (GRCm39) A200T probably damaging Het
Epb41l5 T A 1: 119,551,641 (GRCm39) probably benign Het
Ermp1 A G 19: 29,608,788 (GRCm39) Y164H possibly damaging Het
Gkn2 T C 6: 87,350,401 (GRCm39) probably benign Het
Golgb1 A T 16: 36,739,152 (GRCm39) Q2497L probably damaging Het
Gzme A T 14: 56,355,196 (GRCm39) M245K probably damaging Het
Iars2 A T 1: 185,054,585 (GRCm39) probably benign Het
Icosl T A 10: 77,909,775 (GRCm39) V240D probably damaging Het
Igsf3 T C 3: 101,334,709 (GRCm39) M262T probably benign Het
Ing3 G T 6: 21,971,236 (GRCm39) E336* probably null Het
Kat2a A T 11: 100,597,297 (GRCm39) V625E probably damaging Het
Ksr1 A G 11: 78,929,073 (GRCm39) probably benign Het
Lypd8 A T 11: 58,277,583 (GRCm39) M122L probably benign Het
Mdfi A T 17: 48,143,855 (GRCm39) probably benign Het
Med13 A G 11: 86,192,179 (GRCm39) probably benign Het
Msh6 C T 17: 88,294,112 (GRCm39) R956C probably damaging Het
Myo15b A G 11: 115,774,664 (GRCm39) E670G probably damaging Het
Myo1d A G 11: 80,375,158 (GRCm39) L972P probably damaging Het
Or4s2b A G 2: 88,509,018 (GRCm39) D266G probably damaging Het
Or51ai2 G A 7: 103,587,024 (GRCm39) A146T probably benign Het
Or7g12 T A 9: 18,899,447 (GRCm39) N54K probably benign Het
Pde8b C G 13: 95,244,325 (GRCm39) S143T possibly damaging Het
Pias4 G T 10: 80,993,364 (GRCm39) probably benign Het
Prkca A G 11: 107,872,480 (GRCm39) Y427H probably benign Het
Psg25 G A 7: 18,263,485 (GRCm39) Q113* probably null Het
Rab3gap2 T A 1: 184,982,123 (GRCm39) S392T probably damaging Het
Scrib A G 15: 75,938,756 (GRCm39) probably benign Het
Sdk2 A G 11: 113,793,970 (GRCm39) probably benign Het
Serpinb1c T A 13: 33,070,266 (GRCm39) probably benign Het
Serpinb9f T A 13: 33,511,904 (GRCm39) W136R probably damaging Het
Skic3 C T 13: 76,331,010 (GRCm39) P1480L probably damaging Het
Skint10 C A 4: 112,573,102 (GRCm39) probably benign Het
Slc25a13 T C 6: 6,117,128 (GRCm39) T196A probably damaging Het
Slc26a5 T C 5: 22,052,230 (GRCm39) H33R probably damaging Het
Slc27a6 T C 18: 58,731,829 (GRCm39) probably benign Het
Slitrk6 A T 14: 110,987,251 (GRCm39) Y819N probably damaging Het
Spata46 C T 1: 170,139,603 (GRCm39) Q201* probably null Het
Sptbn1 A T 11: 30,064,739 (GRCm39) V1920E probably damaging Het
Taf6l A G 19: 8,755,881 (GRCm39) F256L probably benign Het
Tmco3 T A 8: 13,342,039 (GRCm39) N104K probably damaging Het
Tmem200c A G 17: 69,149,249 (GRCm39) T611A probably damaging Het
Tmem202 T G 9: 59,432,655 (GRCm39) Y24S probably damaging Het
Tpp1 A G 7: 105,398,626 (GRCm39) L230P probably damaging Het
Trim56 C T 5: 137,141,846 (GRCm39) E557K probably benign Het
Trrap C T 5: 144,790,309 (GRCm39) L3590F probably damaging Het
Tulp4 A G 17: 6,189,387 (GRCm39) T70A possibly damaging Het
Vcp G C 4: 42,986,201 (GRCm39) A297G probably benign Het
Vwf T A 6: 125,603,234 (GRCm39) H861Q probably benign Het
Wdr64 T C 1: 175,599,751 (GRCm39) I536T probably benign Het
Zfp850 T C 7: 27,689,698 (GRCm39) N170S probably benign Het
Zfp87 G A 13: 74,524,544 (GRCm39) probably benign Het
Other mutations in Daw1
AlleleSourceChrCoordTypePredicted EffectPPH Score
IGL00496:Daw1 APN 1 83,174,957 (GRCm39) missense probably damaging 1.00
IGL00717:Daw1 APN 1 83,175,900 (GRCm39) missense probably benign 0.03
IGL01320:Daw1 APN 1 83,175,901 (GRCm39) missense possibly damaging 0.82
IGL01869:Daw1 APN 1 83,159,965 (GRCm39) splice site probably benign
IGL02404:Daw1 APN 1 83,174,952 (GRCm39) missense probably benign 0.15
IGL02516:Daw1 APN 1 83,186,949 (GRCm39) missense probably benign 0.03
IGL02608:Daw1 APN 1 83,187,055 (GRCm39) nonsense probably null
IGL02992:Daw1 APN 1 83,174,934 (GRCm39) splice site probably benign
IGL03015:Daw1 APN 1 83,161,103 (GRCm39) splice site probably benign
IGL03099:Daw1 APN 1 83,157,088 (GRCm39) critical splice donor site probably null
R0050:Daw1 UTSW 1 83,158,086 (GRCm39) missense probably benign 0.01
R0631:Daw1 UTSW 1 83,174,981 (GRCm39) missense probably damaging 1.00
R1420:Daw1 UTSW 1 83,137,548 (GRCm39) missense possibly damaging 0.51
R1678:Daw1 UTSW 1 83,161,087 (GRCm39) missense probably damaging 1.00
R1943:Daw1 UTSW 1 83,186,987 (GRCm39) missense possibly damaging 0.91
R2006:Daw1 UTSW 1 83,169,066 (GRCm39) missense probably damaging 1.00
R2191:Daw1 UTSW 1 83,170,384 (GRCm39) missense probably benign 0.34
R4983:Daw1 UTSW 1 83,165,719 (GRCm39) missense probably benign 0.38
R5129:Daw1 UTSW 1 83,183,624 (GRCm39) missense probably damaging 0.99
R5282:Daw1 UTSW 1 83,170,419 (GRCm39) missense probably benign
R6128:Daw1 UTSW 1 83,183,647 (GRCm39) nonsense probably null
R7438:Daw1 UTSW 1 83,170,436 (GRCm39) missense probably benign
R8888:Daw1 UTSW 1 83,187,011 (GRCm39) missense probably damaging 0.99
R8895:Daw1 UTSW 1 83,187,011 (GRCm39) missense probably damaging 0.99
R8900:Daw1 UTSW 1 83,175,898 (GRCm39) missense probably benign 0.00
R8901:Daw1 UTSW 1 83,183,643 (GRCm39) missense possibly damaging 0.70
Z1088:Daw1 UTSW 1 83,183,685 (GRCm39) missense probably null 1.00
Z1176:Daw1 UTSW 1 83,186,976 (GRCm39) missense probably damaging 1.00
Z1176:Daw1 UTSW 1 83,161,021 (GRCm39) missense probably damaging 1.00
Z1176:Daw1 UTSW 1 83,158,112 (GRCm39) missense probably damaging 0.99
Z1177:Daw1 UTSW 1 83,187,935 (GRCm39) missense unknown
Predicted Primers PCR Primer
(F):5'- GGTAGCACAGGAAGAATATGCCCC -3'
(R):5'- TGTTCAGCAAGCCAGCAGTAACATC -3'

Sequencing Primer
(F):5'- CCTGGAGATAACAGGTTTCTCACAG -3'
(R):5'- CAGTTTAAAAGCCAGTGGCTC -3'
Posted On 2014-08-18