Mutagenetix > Incidental Mutation

Incidental Mutation 'R1508:Vmn2r65'

168064

Institutional Source

Beutler Lab

Gene Symbol

Vmn2r65

Ensembl Gene

ENSMUSG00000066372

Gene Name

vomeronasal 2, receptor 65

Synonyms

ENSMUSG00000070600

MMRRC Submission

040869-MU

Accession Numbers

Essential gene?

Probably non essential (E-score: 0.073) question?

Stock #

R1508 (G1)

Quality Score

225

Status

Not validated

Chromosome

Chromosomal Location

84589377-84613217 bp(-) (GRCm39)

Type of Mutation

missense

DNA Base Change (assembly)

T to A at 84589886 bp (GRCm39)

Zygosity

Heterozygous

Amino Acid Change

Isoleucine to Leucine at position 677 (I677L)

Ref Sequence

ENSEMBL: ENSMUSP00000036551 (fasta)

Gene Model

predicted gene model for transcript(s): [ENSMUST00000044583]

AlphaFold

G3X931

Predicted Effect

probably benign
Transcript: ENSMUST00000044583
AA Change: I677L

PolyPhen 2 Score 0.001 (Sensitivity: 0.99; Specificity: 0.15)

SMART Domains

Protein: ENSMUSP00000036551
Gene: ENSMUSG00000066372
AA Change: I677L

Domain	Start	End	E-Value	Type
signal peptide	1	20	N/A	INTRINSIC
Pfam:ANF_receptor	73	435	5.6e-41	PFAM
Pfam:NCD3G	501	553	1.3e-21	PFAM
Pfam:7tm_3	584	821	2.3e-53	PFAM

Coding Region Coverage

1x: 99.2%
3x: 98.4%
10x: 96.7%
20x: 93.8%

Validation Efficiency

Allele List at MGI

Other mutations in this stock

Total: 51 list
Gene	Ref	Var	Chr/Loc	Mutation	Predicted Effect	Zygosity
Agbl4	T	C	4: 111,513,595 (GRCm39)	I447T	probably benign	Het
Albfm1	A	G	5: 90,729,780 (GRCm39)	E401G	probably benign	Het
Alpk2	C	A	18: 65,482,376 (GRCm39)	G544V	probably damaging	Het
Apob	T	C	12: 8,061,481 (GRCm39)	I3321T	possibly damaging	Het
Ccdc47	C	T	11: 106,093,242 (GRCm39)	D70N	probably damaging	Het
Ceacam13	T	A	7: 17,744,996 (GRCm39)	V22D	possibly damaging	Het
Cep76	A	G	18: 67,756,358 (GRCm39)	M491T	probably damaging	Het
Clec4a3	A	T	6: 122,944,467 (GRCm39)	N171I	probably benign	Het
Col20a1	A	G	2: 180,634,370 (GRCm39)	I112V	probably damaging	Het
Col4a4	A	T	1: 82,433,557 (GRCm39)	L1462H	unknown	Het
Cubn	A	G	2: 13,431,916 (GRCm39)	F1226L	probably benign	Het
Cyp4a31	T	A	4: 115,422,250 (GRCm39)	I77K	possibly damaging	Het
Dcaf1	A	T	9: 106,731,376 (GRCm39)	T618S	probably damaging	Het
Echdc3	A	T	2: 6,211,231 (GRCm39)	S129T	probably benign	Het
Efcab3	T	C	11: 104,601,503 (GRCm39)	V272A	probably benign	Het
Fabp3	C	T	4: 130,206,180 (GRCm39)	T57I	probably benign	Het
Fat1	T	C	8: 45,479,899 (GRCm39)	F2982L	probably benign	Het
Gm9476	A	T	10: 100,142,700 (GRCm39)		noncoding transcript	Het
Golga1	A	G	2: 38,913,261 (GRCm39)	M542T	probably benign	Het
H2-D1	T	A	17: 35,482,844 (GRCm39)	C125S	probably damaging	Het
Jazf1	G	A	6: 52,789,166 (GRCm39)	H103Y	probably damaging	Het
Klhl12	T	C	1: 134,416,712 (GRCm39)	V478A	possibly damaging	Het
Lpcat2b	A	T	5: 107,581,330 (GRCm39)	T220S	probably damaging	Het
Myocd	C	T	11: 65,075,342 (GRCm39)	C594Y	probably damaging	Het
Nf1	A	G	11: 79,331,735 (GRCm39)	E700G	probably damaging	Het
Or52z15	T	C	7: 103,332,678 (GRCm39)	V251A	possibly damaging	Het
Or6k2	T	A	1: 173,986,930 (GRCm39)	M197K	possibly damaging	Het
Papln	A	G	12: 83,829,690 (GRCm39)	Q937R	probably damaging	Het
Pex14	T	C	4: 149,052,029 (GRCm39)	R147G	probably damaging	Het
Plppr3	A	T	10: 79,703,374 (GRCm39)	Y37N	probably damaging	Het
Polr1b	A	G	2: 128,955,654 (GRCm39)	T522A	probably benign	Het
Ppp3cb	T	C	14: 20,574,492 (GRCm39)	N201S	probably damaging	Het
Prf1	A	G	10: 61,139,329 (GRCm39)	D429G	probably damaging	Het
Ptprn2	T	C	12: 117,148,342 (GRCm39)	S701P	probably damaging	Het
Rp1l1	C	T	14: 64,268,341 (GRCm39)	T1309I	possibly damaging	Het
Sipa1l1	T	A	12: 82,487,667 (GRCm39)	M1647K	probably damaging	Het
Skint11	T	C	4: 114,088,963 (GRCm39)		probably null	Het
Slc8a2	T	C	7: 15,874,522 (GRCm39)	Y257H	probably benign	Het
Slfn1	T	A	11: 83,012,181 (GRCm39)	I99N	probably damaging	Het
Slit2	A	G	5: 48,349,591 (GRCm39)	T282A	probably damaging	Het
Slit3	A	T	11: 35,461,448 (GRCm39)	D228V	probably damaging	Het
Specc1l	A	G	10: 75,143,072 (GRCm39)	N1031S	probably benign	Het
Supt6	A	G	11: 78,107,029 (GRCm39)		probably null	Het
Tdrd1	G	A	19: 56,839,790 (GRCm39)	E617K	probably damaging	Het
Tex15	C	G	8: 34,066,880 (GRCm39)	F2103L	probably damaging	Het
Trim21	T	A	7: 102,208,783 (GRCm39)	Q312L	possibly damaging	Het
Trim56	C	T	5: 137,142,791 (GRCm39)	A242T	probably benign	Het
Usp4	T	C	9: 108,249,873 (GRCm39)	V467A	probably benign	Het
Vstm4	T	C	14: 32,585,511 (GRCm39)	V26A	probably damaging	Het
Zbtb14	C	G	17: 69,694,759 (GRCm39)	I152M	probably benign	Het
Zfat	T	A	15: 68,050,600 (GRCm39)	K807I	probably damaging	Het

Other mutations in Vmn2r65

Allele	Source	Chr	Coord	Type	Predicted Effect	PPH Score
IGL00942:Vmn2r65	APN	7	84,592,761 (GRCm39)	missense	probably damaging	0.99
IGL01104:Vmn2r65	APN	7	84,589,996 (GRCm39)	missense	possibly damaging	0.93
IGL01154:Vmn2r65	APN	7	84,592,729 (GRCm39)	missense	probably benign	0.00
IGL01453:Vmn2r65	APN	7	84,589,708 (GRCm39)	missense	probably damaging	1.00
IGL01675:Vmn2r65	APN	7	84,596,587 (GRCm39)	missense	probably benign	0.00
IGL01748:Vmn2r65	APN	7	84,589,507 (GRCm39)	missense	probably damaging	1.00
IGL01997:Vmn2r65	APN	7	84,589,978 (GRCm39)	missense	probably damaging	1.00
IGL02527:Vmn2r65	APN	7	84,595,724 (GRCm39)	missense	possibly damaging	0.82
IGL02617:Vmn2r65	APN	7	84,589,549 (GRCm39)	missense	probably damaging	1.00
IGL02676:Vmn2r65	APN	7	84,589,381 (GRCm39)	missense	possibly damaging	0.86
IGL03084:Vmn2r65	APN	7	84,592,354 (GRCm39)	missense	probably damaging	1.00
A4554:Vmn2r65	UTSW	7	84,595,791 (GRCm39)	missense	probably damaging	0.96
PIT4651001:Vmn2r65	UTSW	7	84,595,461 (GRCm39)	missense	probably benign	0.12
R0322:Vmn2r65	UTSW	7	84,595,756 (GRCm39)	missense	probably benign	0.01
R0453:Vmn2r65	UTSW	7	84,595,442 (GRCm39)	missense	probably benign	0.03
R0862:Vmn2r65	UTSW	7	84,592,853 (GRCm39)	missense	probably benign	0.00
R1392:Vmn2r65	UTSW	7	84,596,624 (GRCm39)	missense	probably benign	0.14
R1392:Vmn2r65	UTSW	7	84,596,624 (GRCm39)	missense	probably benign	0.14
R1687:Vmn2r65	UTSW	7	84,590,026 (GRCm39)	missense	probably benign	0.02
R1876:Vmn2r65	UTSW	7	84,595,505 (GRCm39)	missense	probably damaging	1.00
R2136:Vmn2r65	UTSW	7	84,592,781 (GRCm39)	missense	probably damaging	0.96
R2259:Vmn2r65	UTSW	7	84,590,119 (GRCm39)	missense	possibly damaging	0.86
R2338:Vmn2r65	UTSW	7	84,590,051 (GRCm39)	missense	possibly damaging	0.86
R2880:Vmn2r65	UTSW	7	84,613,094 (GRCm39)	missense	probably damaging	1.00
R3410:Vmn2r65	UTSW	7	84,595,896 (GRCm39)	missense	probably benign	0.00
R3411:Vmn2r65	UTSW	7	84,595,896 (GRCm39)	missense	probably benign	0.00
R3770:Vmn2r65	UTSW	7	84,589,623 (GRCm39)	missense	probably damaging	1.00
R3800:Vmn2r65	UTSW	7	84,589,738 (GRCm39)	missense	possibly damaging	0.93
R3850:Vmn2r65	UTSW	7	84,595,859 (GRCm39)	missense	probably benign	0.00
R4105:Vmn2r65	UTSW	7	84,595,691 (GRCm39)	missense	probably benign	0.03
R4568:Vmn2r65	UTSW	7	84,596,677 (GRCm39)	missense	probably damaging	1.00
R4677:Vmn2r65	UTSW	7	84,613,082 (GRCm39)	missense	possibly damaging	0.55
R4768:Vmn2r65	UTSW	7	84,596,602 (GRCm39)	missense	probably damaging	1.00
R4778:Vmn2r65	UTSW	7	84,592,801 (GRCm39)	missense	possibly damaging	0.47
R5057:Vmn2r65	UTSW	7	84,589,819 (GRCm39)	missense	probably damaging	1.00
R5279:Vmn2r65	UTSW	7	84,589,849 (GRCm39)	missense	probably damaging	1.00
R5280:Vmn2r65	UTSW	7	84,595,542 (GRCm39)	missense	probably damaging	0.99
R5394:Vmn2r65	UTSW	7	84,595,862 (GRCm39)	missense	probably benign	0.04
R5487:Vmn2r65	UTSW	7	84,595,529 (GRCm39)	missense	possibly damaging	0.76
R5580:Vmn2r65	UTSW	7	84,596,802 (GRCm39)	missense	probably damaging	0.99
R5638:Vmn2r65	UTSW	7	84,590,047 (GRCm39)	missense	probably damaging	1.00
R5673:Vmn2r65	UTSW	7	84,596,615 (GRCm39)	missense	probably benign	0.23
R5688:Vmn2r65	UTSW	7	84,589,900 (GRCm39)	missense	probably benign	0.00
R5935:Vmn2r65	UTSW	7	84,592,869 (GRCm39)	missense	probably benign	0.00
R6354:Vmn2r65	UTSW	7	84,589,574 (GRCm39)	missense	probably benign	0.35
R6372:Vmn2r65	UTSW	7	84,589,861 (GRCm39)	missense	probably damaging	1.00
R6924:Vmn2r65	UTSW	7	84,613,198 (GRCm39)	missense	probably benign	0.20
R7021:Vmn2r65	UTSW	7	84,596,587 (GRCm39)	missense	probably benign	0.00
R7195:Vmn2r65	UTSW	7	84,592,347 (GRCm39)	critical splice donor site	probably null
R7422:Vmn2r65	UTSW	7	84,595,569 (GRCm39)	missense	probably damaging	0.99
R7654:Vmn2r65	UTSW	7	84,590,261 (GRCm39)	missense	probably damaging	1.00
R7686:Vmn2r65	UTSW	7	84,589,744 (GRCm39)	missense	probably damaging	0.99
R7691:Vmn2r65	UTSW	7	84,592,851 (GRCm39)	missense	probably benign	0.30
R7798:Vmn2r65	UTSW	7	84,596,192 (GRCm39)	missense	probably damaging	1.00
R7798:Vmn2r65	UTSW	7	84,595,530 (GRCm39)	missense	probably benign	0.00
R8103:Vmn2r65	UTSW	7	84,595,919 (GRCm39)	missense	probably damaging	1.00
R8272:Vmn2r65	UTSW	7	84,596,817 (GRCm39)	missense	probably benign	0.02
R8303:Vmn2r65	UTSW	7	84,589,391 (GRCm39)	nonsense	probably null
R8354:Vmn2r65	UTSW	7	84,589,402 (GRCm39)	missense	possibly damaging	0.73
R8454:Vmn2r65	UTSW	7	84,589,402 (GRCm39)	missense	possibly damaging	0.73
R8489:Vmn2r65	UTSW	7	84,589,964 (GRCm39)	missense	possibly damaging	0.85
R8554:Vmn2r65	UTSW	7	84,595,960 (GRCm39)	missense	probably benign	0.00
R8680:Vmn2r65	UTSW	7	84,589,388 (GRCm39)	missense	probably benign	0.00
R8731:Vmn2r65	UTSW	7	84,589,447 (GRCm39)	nonsense	probably null
R8839:Vmn2r65	UTSW	7	84,595,489 (GRCm39)	nonsense	probably null
R8847:Vmn2r65	UTSW	7	84,590,212 (GRCm39)	missense	probably damaging	1.00
R8916:Vmn2r65	UTSW	7	84,595,665 (GRCm39)	missense	probably benign	0.21
R9254:Vmn2r65	UTSW	7	84,596,650 (GRCm39)	missense	probably damaging	0.99
R9379:Vmn2r65	UTSW	7	84,596,650 (GRCm39)	missense	probably damaging	0.99
R9392:Vmn2r65	UTSW	7	84,589,718 (GRCm39)	missense	possibly damaging	0.95
R9563:Vmn2r65	UTSW	7	84,589,880 (GRCm39)	nonsense	probably null
R9686:Vmn2r65	UTSW	7	84,590,084 (GRCm39)	missense	probably benign	0.45
X0067:Vmn2r65	UTSW	7	84,590,113 (GRCm39)	missense	probably benign	0.04
Z1088:Vmn2r65	UTSW	7	84,592,473 (GRCm39)	critical splice acceptor site	probably null
Z1177:Vmn2r65	UTSW	7	84,590,234 (GRCm39)	missense	probably damaging	0.97

Predicted Primers

PCR Primer
(F):5'- GGGCTAATAAGCCCAAGTAACCCAG -3'
(R):5'- GGCGAATAACCGACTTCTCAGCTAC -3'

Sequencing Primer
(F):5'- AGTAACCCAGTACACAGTAGAATG -3'
(R):5'- AACCGACTTCTCAGCTACATTCTTC -3'

Posted On

2014-04-13